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AB205949

Anti-TMEM24 antibody

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(1 Publication)

Rabbit Polyclonal TMEM24 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human C2CD2L aa 650 to C-terminus.

View Alternative Names

KIAA0285, TMEM24, DLNB23, C2CD2L, Phospholipid transfer protein C2CD2L, C2 domain-containing protein 2-like, Transmembrane protein 24, C2CD2-like

2 Images
Western blot - Anti-TMEM24 antibody (AB205949)
  • WB

Supplier Data

Western blot - Anti-TMEM24 antibody (AB205949)

All lanes:

Western blot - Anti-TMEM24 antibody (ab205949) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 76 kDa

true

Exposure time: 30s

Immunoprecipitation - Anti-TMEM24 antibody (AB205949)
  • IP

Supplier Data

Immunoprecipitation - Anti-TMEM24 antibody (AB205949)

Detection of TMEM24 in Immunoprecipitates of Jurkat whole cell lysates (1 mg for IP, 20% of IP loaded) using ab205949 at 6 μg/mg lysate for IP and 0.4μg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-TMEM24 antibody (ab205949)

Predicted band size: 76 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human C2CD2L aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O14523

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM24 also known as transmembrane protein 24 is an integral membrane protein with approximately 56 kDa mass. It localizes primarily in the endoplasmic reticulum (ER) and is present in a variety of tissues including the brain and pancreas. It plays a role in maintaining lipid homeostasis acting as a lipid transfer protein with the ability to shuttle phosphatidylinositol from the ER to the plasma membrane. TMEM24 has regions that facilitate membrane association and phospholipid transfer which are key for its mechanical function.
Biological function summary

TMEM24 contributes to cellular processes by regulating glucose-stimulated insulin secretion. It does this by forming part of a complex that modulates the availability of membrane lipids necessary for signaling and membrane dynamics. The protein's activity affects the response of pancreatic beta cells influencing insulin release making it an important player in metabolic regulation. TMEM24 also interacts with other proteins involved in lipid metabolism making it essential for energy homeostasis.

Pathways

TMEM24 is important in the insulin signaling and glucose metabolism pathways. It coordinates with proteins such as PI4KIIIβ and E-syts which are important for phosphoinositide lipid synthesis and turnover. These interactions ensure efficient lipid signaling and membrane trafficking contributing to the proper functioning of insulin signaling. TMEM24's role in these pathways underlines its importance in managing cellular lipid levels and energy balance.

TMEM24 has been implicated in metabolic disorders like type 2 diabetes. Its functional interaction with proteins involved in lipid signaling and insulin secretion such as INS and IRS-1 points to its involvement in glucose homeostasis and pancreatic beta-cell response. Alterations in TMEM24 expression or function may lead to impaired insulin secretion and insulin resistance contributing to diabetes pathogenesis. Understanding TMEM24's role in these processes may offer insights into potential therapeutic targets for managing diabetes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lipid-binding protein that transports phosphatidylinositol, the precursor of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), from its site of synthesis in the endoplasmic reticulum to the cell membrane (PubMed : 28209843). It thereby maintains the pool of cell membrane phosphoinositides, which are degraded during phospholipase C (PLC) signaling (PubMed : 28209843). Plays a key role in the coordination of Ca(2+) and phosphoinositide signaling : localizes to sites of contact between the endoplasmic reticulum and the cell membrane, where it tethers the two bilayers (PubMed : 28209843). In response to elevation of cytosolic Ca(2+), it is phosphorylated at its C-terminus and dissociates from the cell membrane, abolishing phosphatidylinositol transport to the cell membrane (PubMed : 28209843). Positively regulates insulin secretion in response to glucose : phosphatidylinositol transfer to the cell membrane allows replenishment of PI(4,5)P2 pools and calcium channel opening, priming a new population of insulin granules (PubMed : 28209843).
See full target information C2CD2L
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 17:e1009285 PubMed33513160

2021

Genome-scale CRISPR screening for modifiers of cellular LDL uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Brian T Emmer,Emily J Sherman,Paul J Lascuna,Sarah E Graham,Cristen J Willer,David Ginsburg
View all publications
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