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AB250454

Anti-TMEM256 antibody [EPR12842] - BSA and Azide free

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Rabbit Recombinant Monoclonal TMEM256 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

C17orf61, TMEM256, Transmembrane protein 256

4 Images
Western blot - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (AB250454)
  • WB

Supplier Data

Western blot - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (AB250454)

This data was developed using ab181239, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TMEM256 antibody [EPR12842] (<a href='/en-us/products/primary-antibodies/tmem256-antibody-epr12842-ab181239'>ab181239</a>) at 1/10000 dilution

Lane 1:

A549 cell lysate at 20 µg

Lane 2:

Y79 cell lysate at 20 µg

Lane 3:

LnCaP cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 12 kDa

false

Immunoprecipitation - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (AB250454)
  • IP

Supplier Data

Immunoprecipitation - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (AB250454)

This data was developed using ab181239, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab181239 at 1/50 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-TMEM256 antibody [EPR12842] (<a href='/en-us/products/primary-antibodies/tmem256-antibody-epr12842-ab181239'>ab181239</a>)

Predicted band size: 12 kDa

false

Flow Cytometry (Intracellular) - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (AB250454)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (AB250454)

This data was developed using ab181239, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of A549 cells (paraformaldehyde-fixed, 2%) labeling TMEM256 with ab181239 at 1/200 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Western blot - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (AB250454)
  • WB

Supplier Data

Western blot - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (AB250454)

This data was developed using ab181239, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TMEM256 antibody [EPR12842] (<a href='/en-us/products/primary-antibodies/tmem256-antibody-epr12842-ab181239'>ab181239</a>) at 1/2000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

Human fetal kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 12 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12842

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250454 is the carrier-free version of ab181239.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM256 also known as Transmembrane Protein 256 is an integral membrane protein with a mass of approximately 18 kDa. It localizes to the cell membrane where it spans the lipid bilayer with multiple transmembrane domains. Expression of TMEM256 is noted in several tissues with higher expression observed in the brain and testis. This suggests a role linked to specific tissue functions possibly related to signaling processes or transport mechanisms across the membrane.
Biological function summary

TMEM256 appears significant in modulating membrane potential and maintaining homeostasis. Though not part of a well-defined complex the protein's characteristics suggest involvement in maintaining cellular ion gradients. This may implicate TMEM256 in key cellular activities such as neuron signaling or other electrically excitable tissue functions. Its role suggests interactions with other membrane proteins which could further modulate its activity or be involved in cellular responses to the external environment.

Pathways

TMEM256 influences cellular signaling and membrane transport mechanisms. It is implicated in ion transport pathways and may interact with proteins like sodium-potassium pumps contributing to maintaining membrane potential. Additionally TMEM256 likely plays a role in neural communication pathways potentially interacting with proteins essential in synaptic function such as neurotransmitter receptors or ion channels suggesting coordination in neuron signaling and rapid response systems.

TMEM256 has links to neurological conditions and reproductive health issues. Aberrant expression or mutations in TMEM256 may connect to neurological disorders potentially through its role in modulating ion gradients and neuron excitability. Another aspect is the possible involvement in reproductive disorders due to its expression patterns in testis. The protein could interact with other disease-related proteins such as ion channels or transporters which are known to be associated with both neurological and reproductive health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information TMEM256
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com