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AB200664

Anti-Tmem27 antibody [EPR17748]

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(2 Publications)

Rabbit Recombinant Monoclonal Tmem27 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

TMEM27, UNQ679/PRO1312, CLTRN, Collectrin, Transmembrane protein 27

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem27 antibody [EPR17748] (AB200664)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem27 antibody [EPR17748] (AB200664)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Tmem27 with ab200664 at 1/10000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Luminal surface and cytoplasm staining on Human kidney tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Tmem27 antibody [EPR17748] (AB200664)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Tmem27 antibody [EPR17748] (AB200664)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling Tmem27 with ab200664 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Cytoplasm staining on BxPC-3 cell line is observed.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab200664 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Tmem27 antibody [EPR17748] (AB200664)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Tmem27 antibody [EPR17748] (AB200664)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human embryonic kidney) cells labeling Tmem27 with ab200664 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Cytoplasm staining on HEK293 cell line is observed.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab200664 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem27 antibody [EPR17748] (AB200664)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem27 antibody [EPR17748] (AB200664)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Tmem27 with ab200664 at 1/10000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

No staining on Human cerebral cortex tissue is observed (Negative control for Tmem27).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Tmem27 antibody [EPR17748] (AB200664)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Tmem27 antibody [EPR17748] (AB200664)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling Tmem27 with ab200664 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730;black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem27 antibody [EPR17748] (AB200664)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem27 antibody [EPR17748] (AB200664)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Tmem27 with ab200664 at 1/10000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Luminal surface and cytoplasm staining on rat kidney tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-Tmem27 antibody [EPR17748] (AB200664)
  • WB

Supplier Data

Western blot - Anti-Tmem27 antibody [EPR17748] (AB200664)

The observed MW is consistent with what has been described in the literature PMID : 16330323.

Please note lanes 2-4 are negative controls for Tmem27.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Tmem27 antibody [EPR17748] (ab200664) at 1/1000 dilution

Lane 1:

Human pancreas lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Lane 4:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 38 kDa

false

Exposure time: 3min

Western blot - Anti-Tmem27 antibody [EPR17748] (AB200664)
  • WB

Unknown

Western blot - Anti-Tmem27 antibody [EPR17748] (AB200664)

The observed MW is consistent with what has been described in the literature PMID : 16330323.

Please note lanes 2-4 are negative controls for Tmem27.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Tmem27 antibody [EPR17748] (ab200664) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Lane 4:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 19 kDa,25 kDa

Observed band size: 17 kDa,38 kDa

false

Exposure time: 5s

Western blot - Anti-Tmem27 antibody [EPR17748] (AB200664)
  • WB

Supplier Data

Western blot - Anti-Tmem27 antibody [EPR17748] (AB200664)

The observed MW is consistent with what has been described in the literature PMID : 16330323.

Please note lanes 2-4 and 6-8 are negative controls for Tmem27.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Tmem27 antibody [EPR17748] (ab200664) at 1/1000 dilution

Lane 1:

Mouse kidney lysate at 10 µg

Lane 2:

Mouse brain lysate at 10 µg

Lane 3:

Mouse heart lysate at 10 µg

Lane 4:

Mouse spleen lysate at 10 µg

Lane 5:

Rat kidney lysate at 10 µg

Lane 6:

Rat brain lysate at 10 µg

Lane 7:

Rat heart lysate at 10 µg

Lane 8:

Rat spleen lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 38 kDa

false

Exposure time: 5s

  • Carrier free

    Anti-Tmem27 antibody [EPR17748] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17748

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Reagents, Controls & Accessories

AB93684

Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tmem27 also known as collectrin is a type I transmembrane protein with a mass of approximately 46 kDa. It is predominantly expressed in the kidney and pancreas where it localizes mostly at the apical membranes of epithelial cells in renal proximal tubules and pancreatic ducts. Tmem27 engages in processes associated with cell surface expression influencing the behavior of nearby ion channels and transporters.
Biological function summary

The involvement of Tmem27 extends to the regulation of amino acid transport and insulin exocytosis. It associates with the γ-aminobutyric acid type B receptor (GABBR) complex contributing to the modulation of nutrient uptake. Tmem27 influences the processing and trafficking of small neutral amino acids enhancing their absorption across epithelial barriers which is important for maintaining cellular homeostasis and metabolic activities.

Pathways

The function of Tmem27 is significant in the renin-angiotensin system and the insulin signaling pathway. In the renin-angiotensin system Tmem27 operates alongside the angiotensin-converting enzyme (ACE) to precisely regulate blood pressure homeostasis. It also acts within the insulin signaling pathway by modulating insulin secretion potentially working closely with glucose transporter type 4 (GLUT4) to ensure efficient glucose uptake by cells.

Tmem27 has connections to conditions such as diabetes mellitus and kidney disease. Its involvement in insulin secretion links it to diabetes impacting glucose homeostasis when irregularities occur. Tmem27's interactions with angiotensin-converting enzyme can affect blood pressure regulation creating implications for hypertension-related kidney disorders. Understanding these associations highlights the potential of Tmem27 as a therapeutic target in managing these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in amino acid transport by acting as binding partner of amino acid transporters SLC6A18 and SLC6A19, regulating their trafficking on the cell surface and their amino acid transporter activity (By similarity). May also play a role in trafficking of amino acid transporters SLC3A1 and SLC7A9 to the renal cortical cell membrane (By similarity). Regulator of SNARE complex function (PubMed : 16330323). Stimulator of beta cell replication (PubMed : 16330323).
See full target information CLTRN
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 298:102585 PubMed36223838

2022

Tick-borne encephalitis virus capsid protein induces translational shutoff as revealed by its structural-biological analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Selinger,Radim Novotný,Jakub Sýs,Justin A Roby,Hana Tykalová,Ganji Sri Ranjani,Marie Vancová,Kateřina Jaklová,Filip Kaufman,Marshall E Bloom,Zbyněk Zdráhal,Libor Grubhoffer,Jade K Forwood,Richard Hrabal,Michaela Rumlová,Ján Štěrba

Anticancer research 38:5217-5222 PubMed30194170

2018

Shift of Keratin Expression Profile in End-stage Kidney Increases the Risk of Tumor Development.

Applications

Unspecified application

Species

Unspecified reactive species

Donat Peter Sarlos,Lehel Peterfi,Arpad Szanto,Gyula Kovacs
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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