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AB185944

Anti-TMEM30B antibody [EPR14409]

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(2 Publications)

Rabbit Recombinant Monoclonal TMEM30B antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

CDC50B, TMEM30B, Cell cycle control protein 50B, P4-ATPase flippase complex beta subunit TMEM30B, Transmembrane protein 30B

2 Images
Western blot - Anti-TMEM30B antibody [EPR14409] (AB185944)
  • WB

Supplier Data

Western blot - Anti-TMEM30B antibody [EPR14409] (AB185944)

All lanes:

Western blot - Anti-TMEM30B antibody [EPR14409] (ab185944) at 1/1000 dilution

Lane 1:

rat kidney lysate at 10 µg

Lane 2:

mouse Raw264.7 cell lysate at 10 µg

Lane 3:

mouse NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa

true

Western blot - Anti-TMEM30B antibody [EPR14409] (AB185944)
  • WB

Supplier Data

Western blot - Anti-TMEM30B antibody [EPR14409] (AB185944)

All lanes:

Western blot - Anti-TMEM30B antibody [EPR14409] (ab185944) at 1/10000 dilution

Lane 1:

BxPC-3 cell lysate at 20 µg

Lane 2:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14409

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM30B also known as CDC50B is a regulatory subunit of P4-ATPase flippases. This protein interacts with P4-ATPases to facilitate the transport of phospholipids from the outer to the inner leaflet of the plasma membrane. TMEM30B has a molecular mass of approximately 38 kDa. It expresses highly in brain tissue with some expression noted in the heart and skeletal muscles as well. Understanding its mechanical role helps us appreciate its importance in maintaining proper cell membrane function and integrity.
Biological function summary

TMEM30B serves as an essential component of the P4-ATPase complex playing a role in maintaining lipid asymmetry in cell membranes. This lipid asymmetry is critical for various cellular processes including vesicular trafficking and cell signaling. TMEM30B is involved in cellular processes such as neuronal development and synaptic function given its higher expression in brain tissue. The existence of TMEM30B within this complex helps maintain the delicate balance required for functional and structural stability of cell membranes.

Pathways

TMEM30B is involved in lipid metabolism and cellular homeostasis pathways. The protein's interaction with P4-ATPases like ATP8A2 plays a role in the maintenance of phospholipid distribution across the plasma membrane an important aspect of lipid metabolism. TMEM30B is also associated with the endocytosis pathway influencing how cells internalize substances and respond to external signals. It functions alongside proteins such as CDC50A in these pathways to ensure efficient cellular operations.

TMEM30B shows a relationship with neurological disorders including neurodevelopmental disorders and epilepsy. Abnormal expression or mutations in TMEM30B disrupt lipid asymmetry in neuronal cells impacting membrane fluidity and signaling leading to disease symptoms. In addition TMEM30B associates with Alzheimer's disease where it affects the amyloid precursor protein (APP) processing potentially contributing to plaque formation. Understanding these connections can lead to better therapeutic strategies targeting TMEM30B-related pathways and diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accessory component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation also seems to be implicated in vesicle formation and in uptake of lipid signaling molecules. The beta subunit may assist in binding of the phospholipid substrate (Probable). Can mediate the export of alpha subunits ATP8A1, ATP8B1, ATP8B2 and ATP8B4 from the ER to the plasma membrane.
See full target information TMEM30B
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6391 PubMed40640125

2025

Super-resolution microscopy of mitochondrial mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Stoldt,Frederike Maass,Michael Weber,Sven Dennerlein,Peter Ilgen,Jutta Gärtner,Aysenur Canfes,Sarah V Schweighofer,Daniel C Jans,Peter Rehling,Stefan Jakobs

International journal of oral science 16:59 PubMed39384739

2024

CREB3L1 deficiency impairs odontoblastic differentiation and molar dentin deposition partially through the TMEM30B.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Li,Yuxiu Lin,Jinqiang Guo,Delan Huang,Huanyan Zuo,Hanshu Zhang,Guohua Yuan,Huan Liu,Zhi Chen
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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