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Rabbit Recombinant Monoclonal TM41B antibody. Suitable for WB, IP and reacts with Human samples.

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Images

Western blot - Anti-TMEM41B antibody [EPR28149-41] (AB314763), expandable thumbnail
  • Western blot - Anti-TMEM41B antibody [EPR28149-41] (AB314763), expandable thumbnail
  • Immunoprecipitation - Anti-TMEM41B antibody [EPR28149-41] (AB314763), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PFlow Cyt (Intra)ICC/IF
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Phospholipid scramblase involved in lipid homeostasis and membrane dynamics processes (PubMed:33850023, PubMed:33929485, PubMed:34015269). Has phospholipid scramblase activity toward cholesterol and phosphatidylserine, as well as phosphatidylethanolamine and phosphatidylcholine (PubMed:33850023, PubMed:33929485, PubMed:34015269). Required for autophagosome formation: participates in early stages of autophagosome biogenesis at the endoplasmic reticulum (ER) membrane by reequilibrating the leaflets of the ER as lipids are extracted by ATG2 (ATG2A or ATG2B) to mediate autophagosome assembly (PubMed:30093494, PubMed:30126924, PubMed:30933966, PubMed:33850023, PubMed:33929485, PubMed:34015269, PubMed:34043740). In addition to autophagy, involved in other processes in which phospholipid scramblase activity is required (PubMed:33850023). Required for normal motor neuron development (By similarity). (Microbial infection) Critical host factor required for infection by human coronaviruses SARS-CoV-2, HCoV-OC43, HCoV-NL63, and HCoV-229E, as well as all flaviviruses tested such as Zika virus and Yellow fever virus (PubMed:33338421, PubMed:33382968). Required post-entry of the virus to facilitate the ER membrane remodeling necessary to form replication organelles (PubMed:33382968).

Alternative names

KIAA0033, TMEM41B, Transmembrane protein 41B, Protein stasimon

Recommended products

Rabbit Recombinant Monoclonal TM41B antibody. Suitable for WB, IP and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28149-41
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TMEM41B also known as Transmembrane Protein 41B is part of the ER membrane protein complex that plays a fundamental role in various cellular processes. It is a 35 kDa protein located predominantly in the endoplasmic reticulum membrane. TMEM41B shows expression in many tissues including heart brain kidney and skeletal muscle suggesting its important role across different biological systems.

Biological function summary

This protein functions in autophagy a process essential for cellular homeostasis and survival during stress conditions. TMEM41B associates with the ER-phagy complex where it is important for the regulation and expansion of autophagosomes. It works in coordination with proteins like VMP1 and ATG9A supporting the recruitment and stability of autophagosomal membranes which impacts the cell's ability to recycle and reuse cellular components.

Pathways

TMEM41B has a significant involvement in the autophagy and lipid metabolism pathways. It interacts with other proteins such as VMP1 and ATG9A mediating the lipid mobilization necessary for efficient autophagosome formation. These interactions ensure that TMEM41B plays a role in the homeostatic regulation of lipids and proteins contributing to proper cellular function and response to metabolic demands.

Associated diseases and disorders

TMEM41B associates with conditions like neurodegeneration and certain viral infections. Alterations or deficiencies in TMEM41B can impact its autophagic function potentially leading to neurodegenerative diseases where protein aggregates accumulate as seen in relation to ATG9A dysfunction. Additionally studies link TMEM41B to the flavivirus life cycle suggesting its necessity in viral replication processes. These connections highlight its potential as a therapeutic target in disease management.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-TMEM41B antibody [EPR28149-41] (ab314763), expandable thumbnail

    Western blot - Anti-TMEM41B antibody [EPR28149-41] (ab314763)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-TMEM41B antibody [EPR28149-41] (ab314763) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: 293T transfected with siRNAs specifically targeting TMEM41B whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 32 kDa

    Exposure time: 180s

  • Western blot - Anti-TMEM41B antibody [EPR28149-41] (ab314763), expandable thumbnail

    Western blot - Anti-TMEM41B antibody [EPR28149-41] (ab314763)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.
    Exposure time: Lane 1:180 seconds
    Lane 2: 59 seconds
    Lane 3: 26 seconds

    All lanes: Western blot - Anti-TMEM41B antibody [EPR28149-41] (ab314763) at 1/1000 dilution

    Lane 1: NCI-H1299 (human lu carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HCT-15 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 32 kDa

  • Immunoprecipitation - Anti-TMEM41B antibody [EPR28149-41] (ab314763), expandable thumbnail

    Immunoprecipitation - Anti-TMEM41B antibody [EPR28149-41] (ab314763)

    TMEM41B was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) whole cell lysate with ab314763 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314763 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 2: ab314763 IP in 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314763 in 293T whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-TMEM41B antibody [EPR28149-41] (ab314763) at 1/30 dilution

    All lanes: 293T (human embryonic kidney epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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