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Rabbit Recombinant Monoclonal TMEM43 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-TMEM43 antibody [EPR15378(B)] - BSA and Azide free (AB250723), expandable thumbnail
  • Western blot - Anti-TMEM43 antibody [EPR15378(B)] - BSA and Azide free (AB250723), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM43 antibody [EPR15378(B)] - BSA and Azide free (AB250723), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM43 antibody [EPR15378(B)] - BSA and Azide free (AB250723), expandable thumbnail
  • Western blot - Anti-TMEM43 antibody [EPR15378(B)] - BSA and Azide free (AB250723), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

May have an important role in maintaining nuclear envelope structure by organizing protein complexes at the inner nuclear membrane. Required for retaining emerin at the inner nuclear membrane (By similarity). Plays a role in the modulation of innate immune signaling through the cGAS-STING pathway by interacting with RNF26 (PubMed:32614325). In addition, functions as a critical signaling component in mediating NF-kappa-B activation by acting downstream of EGFR and upstream of CARD10 (PubMed:27991920). Contributes to passive conductance current in cochlear glia-like supporting cells, mediated by gap junctions and necessary for hearing and speech discrimination (PubMed:34050020).

Alternative names

UNQ2564/PRO6244, TMEM43, Transmembrane protein 43, Protein LUMA

Recommended products

Rabbit Recombinant Monoclonal TMEM43 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR15378(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250723 is the carrier-free version of Anti-TMEM43 antibody [EPR15378(B)] ab184164.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TMEM43 also known as LUMA is a transmembrane protein with a mass of approximately 47 kDa. This protein localizes mainly to the nuclear envelope where it plays a structural role in maintaining nuclear integrity. It is widely expressed in various tissues especially in cardiac and skeletal muscle cells. Through its location and structure TMEM43 contributes to the physical and functional stability of the nuclear envelope interacting with other nuclear envelope components.

Biological function summary

Researchers recognize TMEM43 as an important player in the integrity of cellular architectures maintaining nuclear shape and mechanical stability. It forms part of a larger protein complex within the nuclear envelope referred to as the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex. The interactions between TMEM43 and other LINC complex proteins contribute to its role in cellular mechanical transduction and nuclear-cytoskeletal coupling essential for cellular responses.

Pathways

TMEM43 plays a significant role in pathways related to nuclear-cytoskeletal interaction and mechanotransduction. It associates with the LINC complex proteins like Emerin and Lamin A/C facilitating the integration of signals from the cytoskeleton to the nucleus. This involvement places TMEM43 within the context of mechanosensitive pathways important for cellular responses to mechanical stimuli which are relevant in tissue development and homeostasis.

Associated diseases and disorders

TMEM43 links closely to certain cardiac conditions and muscular disorders. Mutations in this protein are known to cause arrhythmogenic right ventricular cardiomyopathy (ARVC) a life-threatening heart disease characterized by fibro-fatty degeneration of the right ventricle. This connection also implicates proteins like Desmoplakin and Plakophilin-2 in ARVC as they form part of the complex that interacts with TMEM43. Additionally TMEM43's dysfunction may relate to muscular disorders where altered interactions with members of the nuclear envelope proteins contribute to muscle weakness and degeneration.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-TMEM43 antibody [EPR15378(B)] - BSA and Azide free (ab250723), expandable thumbnail

    Western blot - Anti-TMEM43 antibody [EPR15378(B)] - BSA and Azide free (ab250723)

    This data was developed using Anti-TMEM43 antibody [EPR15378(B)] ab184164, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-TMEM43 antibody [EPR15378(B)] (Anti-TMEM43 antibody [EPR15378(B)] ab184164) at 1/5000 dilution

    Lane 1: Human spleen tissue lysate at 20 µg

    Lane 2: Human placenta tissue lysate at 20 µg

    Lane 3: MDA-MB-435 cell lysate at 20 µg

    Lane 4: Jurkat cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa

  • Western blot - Anti-TMEM43 antibody [EPR15378(B)] - BSA and Azide free (ab250723), expandable thumbnail

    Western blot - Anti-TMEM43 antibody [EPR15378(B)] - BSA and Azide free (ab250723)

    This data was developed using Anti-TMEM43 antibody [EPR15378(B)] ab184164, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-TMEM43 antibody [EPR15378(B)] (Anti-TMEM43 antibody [EPR15378(B)] ab184164) at 1/20000 dilution

    All lanes: Caco-2 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM43 antibody [EPR15378(B)] - BSA and Azide free (ab250723), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM43 antibody [EPR15378(B)] - BSA and Azide free (ab250723)

    This data was developed using Anti-TMEM43 antibody [EPR15378(B)] ab184164, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TMEM43 with Anti-TMEM43 antibody [EPR15378(B)] ab184164 at 1/500 dilution. The slide is counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM43 antibody [EPR15378(B)] - BSA and Azide free (ab250723), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM43 antibody [EPR15378(B)] - BSA and Azide free (ab250723)

    This data was developed using Anti-TMEM43 antibody [EPR15378(B)] ab184164, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TMEM43 with Anti-TMEM43 antibody [EPR15378(B)] ab184164 at 1/500 dilution. The slide is counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-TMEM43 antibody [EPR15378(B)] - BSA and Azide free (ab250723), expandable thumbnail

    Western blot - Anti-TMEM43 antibody [EPR15378(B)] - BSA and Azide free (ab250723)

    This data was developed using Anti-TMEM43 antibody [EPR15378(B)] ab184164, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-TMEM43 antibody [EPR15378(B)] (Anti-TMEM43 antibody [EPR15378(B)] ab184164) at 1/5000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Lane 3: Mouse kidney tissue lysate at 20 µg

    Lane 4: Mouse spleen tissue lysate at 20 µg

    Lane 5: Rat brain tissue lysate at 20 µg

    Lane 6: Rat heart tissue lysate at 20 µg

    Lane 7: Rat kidney tissue lysate at 20 µg

    Lane 8: Rat spleen tissue lysate at 20 µg

    Lane 9: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 10: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 11: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Lane 12: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 45 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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