Rabbit Polyclonal TMEM49/VMP1 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human VMP1 aa 350 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human VMP1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96GC9
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Predicted | Predicted |
| Rat | Tested | Expected |
| Cow | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Phospholipid scramblase involved in lipid homeostasis and membrane dynamics processes (PubMed:33850023, PubMed:33929485). Has phospholipid scramblase activity toward cholesterol and phosphatidylserine, as well as phosphatidylethanolamine and phosphatidylcholine (PubMed:33850023, PubMed:33929485). Required for autophagosome formation: participates in early stages of autophagosome biogenesis at the endoplasmic reticulum (ER) membrane by reequilibrating the leaflets of the ER as lipids are extracted by ATG2 (ATG2A or ATG2B) to mediate autophagosome assembly (PubMed:28890335, PubMed:30093494, PubMed:30933966, PubMed:33850023, PubMed:33929485). Regulates ATP2A2 activity to control ER-isolation membrane contacts for autophagosome formation (PubMed:28890335). In addition to autophagy, involved in other processes in which phospholipid scramblase activity is required (PubMed:31526472, PubMed:33850023). Modulates ER contacts with lipid droplets, mitochondria and endosomes (PubMed:28890335). Plays an essential role in formation of cell junctions (PubMed:17724469). Upon stress such as bacterial and viral infection, promotes formation of cytoplasmic vacuoles followed by cell death (By similarity). Involved in the cytoplasmic vacuolization of acinar cells during the early stage of acute pancreatitis (By similarity). (Microbial infection) Host factor required for infection by all flaviviruses tested such as Zika virus and Yellow fever virus (PubMed:33338421). Probably required post-entry of the virus to facilitate the ER membrane remodeling necessary to form replication organelles (PubMed:33338421).
Alternative names
TDC1, TMEM49, HSPC292, VMP1, Vacuole membrane protein 1, Transmembrane protein 49
Recommended products
Rabbit Polyclonal TMEM49/VMP1 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human VMP1 aa 350 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human VMP1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96GC9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TMEM49 also known as VMP1 or Vacuole Membrane Protein 1 is an integral membrane protein with a molecular mass of approximately 52 kDa. It is located primarily within the endoplasmic reticulum and the membranes of various organelles. TMEM49/VMP1 exhibits wide expression across various tissues including the pancreas heart and skeletal muscles indicating a broad functional role in cellular processes linked to these tissues.
- Biological function summary
The TMEM49/VMP1 protein plays a significant role in autophagy a cellular degradation process important for maintaining cell health. This protein is part of a complex that interacts with Beclin-1 an important player in the initiation of autophagy. By promoting the formation of autophagosomes TMEM49/VMP1 ensures the efficient degradation of unnecessary or damaged cellular components therefore facilitating cellular homeostasis and energy balance.
- Pathways
TMEM49/VMP1 is important for proper functionality in autophagic and pancreatic secretory pathways. In the context of autophagy VMP1 collaborates with proteins such as LC3 and ATG proteins to progress the autophagic process. Meanwhile in pancreatic secretory pathways TMEM49/VMP1 interacts with the early secretory pathway influencing exocytosis. These pathways interact and share components highlighting the role VMP1 plays in essential cellular functions.
- Associated diseases and disorders
TMEM49/VMP1 has connections with pancreatitis and certain types of cancer. Mutations or dysregulation in VMP1 expression can contribute to the development of pancreatitis potentially through disturbances in autophagy and exocrine functions. In cancer abnormal autophagy regulation sometimes involving the VMP1-Beclin-1 complex may facilitate cancer cell survival. Understanding these interactions is important for unveiling new therapeutic targets and developing novel treatment strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-TMEM49/VMP1 antibody - C-terminal (ab240887)
Immunocytochemistry/Immunofluorescence analysis of RKO (Human colon carcinoma cell line) cells labeling TMEM49/VMP1 using ab240887 at 1:100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at RT.
Counterstain: Phalloidin Texas Red F-Actin stain at 1:1000 dilution for 60 min at RT; DAPI (blue) nuclear stain at 1:5000 for 5 min at RT.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab240887 (D) Composite.
Western blot - Anti-TMEM49/VMP1 antibody - C-terminal (ab240887)
Block: 5% skimmed milk in 1X TBST.Color development: TMB.
All lanes: 16 hours at 4°C at 1/1000 dilution
All lanes: Rat pancreas cell lysate at 15 µg
SecondaryAll lanes: Goat-Anti-Rabbit IgG: HRP for 60 minutes at RT at 1/200 dilution
Predicted band size: 46 kDa
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