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AB97597

Anti-TMEM59 antibody

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(2 Publications)

Rabbit Polyclonal TMEM59 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TMEM59 aa 1-300.

View Alternative Names

C1orf8, HSPC001, UNQ169/PRO195, TMEM59, Transmembrane protein 59, Liver membrane-bound protein

4 Images
Immunohistochemistry paraffin embedded sections - Anti-TMEM59 antibody (AB97597)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-TMEM59 antibody (AB97597)

ab97597 detects TMEM59 protein at on mouse lung by immunohistochemical analysis.
Paraffin-embedded mouse lung stained with ab97597 at dilution 1/500.
Antigen Retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western blot - Anti-TMEM59 antibody (AB97597)
  • WB

Unknown

Western blot - Anti-TMEM59 antibody (AB97597)

12% SDS PAGE

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Western blot - Anti-TMEM59 antibody (ab97597) at 1/500 dilution

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A431 whole cell lysate at 30 µg

Predicted band size: 36 kDa

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Western blot - Anti-TMEM59 antibody (AB97597)
  • WB

Supplier Data

Western blot - Anti-TMEM59 antibody (AB97597)

ab97597 antibody detects TMEM59 protein by Western blot analysis.

Wester blot was performed on 10% SDS-PAGE of 50 μg rat kidney lysate/extract.

All lanes:

Western blot - Anti-TMEM59 antibody (ab97597) at 1/1000 dilution

All lanes:

Rat kidney lysate/extract at 50 µg

Predicted band size: 36 kDa

false

Western blot - Anti-TMEM59 antibody (AB97597)
  • WB

Unknown

Western blot - Anti-TMEM59 antibody (AB97597)

All lanes:

Western blot - Anti-TMEM59 antibody (ab97597)

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Mouse liver lysate at 50 µg

Predicted band size: 36 kDa

Observed band size: 36 kDa,53 kDa,60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human TMEM59 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9BXS4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry paraffin embedded sections", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM59 also known as Transmembrane Protein 59 plays a role in protein glycosylation and modulation of the cell surface expression of certain proteins. It has a molecular mass of approximately 39 kDa. TMEM59 is expressed in a variety of tissues including the brain lung and kidney where it localizes to the endoplasmic reticulum and Golgi apparatus. The protein's structure suggests membrane spanning properties that contribute to its function in cell regulation.
Biological function summary

TMEM59 contributes to proper neuronal function by modulating the cell surface expression of proteins involved in neuron-neuron communications. This regulation has implications for membrane protein function and synaptic stability. TMEM59 participates in certain bionetworks interacting with protein complexes that influence cell signaling pathways. Such interactions play significant roles in controlling protein trafficking and localization within cells impacting the cellular response to environmental signals.

Pathways

TMEM59 influences processes like the Wnt signaling pathway affecting cell fate decisions and embryonic development. It interacts with proteins such as β-catenin and affects their stability and function. TMEM59 also participates in the autophagy-dependent pathways modulating cellular mechanisms involved in the degradation of damaged proteins and organelles therefore maintaining cellular homeostasis.

TMEM59 has links to neurodegenerative diseases including Alzheimer's disease and schizophrenia. TMEM59's regulation of proteins like APP (Amyloid precursor protein) is significant. Disruptions in its function may contribute to aberrant protein aggregation commonly seen in Alzheimer's disease pathology. Additionally disruptions in TMEM59 expression or function could affect cognitive processes and synaptic connections related to schizophrenia as it influences the trafficking of receptor proteins important for synaptic signaling.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a regulator of autophagy in response to S.aureus infection by promoting activation of LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C). Acts by interacting with ATG16L1, leading to promote a functional complex between LC3 and ATG16L1 and promoting LC3 lipidation and subsequent activation of autophagy (PubMed : 23376921, PubMed : 27273576). Modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP (PubMed : 20427278). Inhibits APP transport to the cell surface and further shedding (PubMed : 20427278).
See full target information TMEM59
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:6332 PubMed34732708

2021

Progenitor translatome changes coordinated by Tsc1 increase perception of Wnt signals to end nephrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Alison E Jarmas,Eric W Brunskill,Praneet Chaturvedi,Nathan Salomonis,Raphael Kopan

Oncotarget 8:19855-19865 PubMed28423639

2017

M2 microglia promotes neurogenesis and oligodendrogenesis from neural stem/progenitor cells via the PPARγ signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jichao Yuan,Hongfei Ge,Wei Liu,Haitao Zhu,Yaxing Chen,Xuan Zhang,Yang Yang,Yi Yin,Weixiang Chen,Wanjiang Wu,Yunfeng Yang,Jiangkai Lin
View all publications
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