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AB250825

Anti-TMEM59L antibody [EPR14975] - BSA and Azide free

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(2 Publications)

Rabbit Recombinant Monoclonal TMEM59L antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

BSMAP, C19orf4, TMEM59L, Transmembrane protein 59-like, Brain-specific membrane-anchored protein

2 Images
Western blot - Anti-TMEM59L antibody [EPR14975] - BSA and Azide free (AB250825)
  • WB

Supplier Data

Western blot - Anti-TMEM59L antibody [EPR14975] - BSA and Azide free (AB250825)

This data was developed using ab185227, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TMEM59L antibody [EPR14975] (<a href='/en-us/products/primary-antibodies/tmem59l-antibody-epr14975-ab185227'>ab185227</a>) at 1/2000 dilution

All lanes:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

Western blot - Anti-TMEM59L antibody [EPR14975] - BSA and Azide free (AB250825)
  • WB

Supplier Data

Western blot - Anti-TMEM59L antibody [EPR14975] - BSA and Azide free (AB250825)

This data was developed using ab185227, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TMEM59L antibody [EPR14975] (<a href='/en-us/products/primary-antibodies/tmem59l-antibody-epr14975-ab185227'>ab185227</a>) at 1/10000 dilution

All lanes:

Human cerebellum cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14975

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

AB97597

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Immunohistochemistry paraffin embedded sections - Anti-TMEM59 antibody (AB97597)

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Primary Antibodies

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Primary Antibodies

AB109235

Anti-Xanthine Oxidase antibody [EPR4605]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Xanthine Oxidase antibody [EPR4605] (AB109235)

  • 4
  • 3
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250825 is the carrier-free version of ab185227.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM59L also known as Transmembrane Protein 59-Like is a protein with an approximate mass of 43 kDa. This protein is usually expressed in the brain with higher levels detected in regions like the cerebral cortex and hippocampus. TMEM59L is a type I transmembrane protein which means it spans the cellular membrane once and its N-terminal is inside the extracellular space. Its structure suggests a role in cellular signaling and membrane dynamics although its distinct functional domains are not entirely mapped yet.
Biological function summary

TMEM59L acts in neural differentiation and synaptic function. The protein influences neuronal development and may also play a role in synapse formation and maintenance. It is not known to be part of a larger multiprotein complex strictly but it interacts with several cellular components involved in neuronal plasticity. The presence of TMEM59L in neuronal tissue suggests that it contributes significantly to processes integral to cognitive functions.

Pathways

TMEM59L participates in cerebral processes involving cellular communication and neural integrity. It interacts within the Wnt signaling pathway which is important for neurite outgrowth and synaptic plasticity. This pathway involves other proteins such as Disheveled (DVL) and beta-catenin which further highlight the role of TMEM59L in neural connectivity and growth. In addition TMEM59L's interactions may influence pathways related to receptor trafficking connecting it to cytoskeleton-associated proteins.

TMEM59L links to neurodegenerative conditions including Alzheimer's disease and cognitive decline. In Alzheimer's disease irregularities in the expression or function of TMEM59L might disturb normal synaptic activity or neuronal health. This protein relates to beta-amyloid precursor protein (APP) which plays a role in disease pathology. Changes in TMEM59L could also affect neural plasticity leading to cognitive impairments suggesting that it might serve as a biomarker or therapeutic target in addressing such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of APP. Inhibits APP transport to the cell surface and further shedding.
See full target information TMEM59L
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu

The American journal of pathology 187:1686-1699 PubMed28618255

2017

Osteocyte Regulation of Receptor Activator of NF-κB Ligand/Osteoprotegerin in a Sheep Model of Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Thaqif El Khassawna,Felix Merboth,Deeksha Malhan,Wolfgang Böcker,Diaa E S Daghma,Sabine Stoetzel,Stefanie Kern,Fathi Hassan,Dirk Rosenbaum,Judith Langenstein,Natali Bauer,Anja Schlagenhauf,Angela Rösen-Wolff,Felix Schulze,Markus Rupp,Dirk Hose,Anja Secklinger,Anita Ignatius,Hans-Joachim Wilke,Katrin S Lips,Christian Heiss
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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