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AB236861

Anti-TMEM65 antibody

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(2 Publications)

Rabbit Polyclonal TMEM65 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TMEM65.

View Alternative Names

Transmembrane protein 65, TMEM65

2 Images
Western blot - Anti-TMEM65 antibody (AB236861)
  • WB

Supplier Data

Western blot - Anti-TMEM65 antibody (AB236861)

All lanes:

Western blot - Anti-TMEM65 antibody (ab236861) at 1/500 dilution

Lane 1:

Mouse liver lysate

Lane 2:

Mouse kidney lysate

Lane 3:

Mouse gonadal lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 25 kDa

false

Western blot - Anti-TMEM65 antibody (AB236861)
  • WB

Supplier Data

Western blot - Anti-TMEM65 antibody (AB236861)

All lanes:

Western blot - Anti-TMEM65 antibody (ab236861) at 1/1000 dilution

Lane 1:

PC-3 whole cell lysate

Lane 2:

Hela whole cell lysate

Lane 3:

MCF-7 whole cell lysate

Lane 4:

293 whole cell lysate

Lane 5:

SH-SY5Y whole cell lysate

Lane 6:

Mouse brain tissue

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 25 kDa

Observed band size: 20-25 kDa

false

Exposure time: 5min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TMEM65.

Q6PI78

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM65 also known as Transmembrane protein 65 is a protein with a mass of approximately 27 kDa. It is a component of mitochondria largely expressed in cardiac and skeletal muscles. TMEM65 resides in the inner mitochondrial membrane where it plays a role in maintaining the structural integrity of the organelle. Its function is linked to the transmembrane transport of ions and metabolites contributing to mitochondrial efficiency.
Biological function summary

TMEM65 influences cellular energy dynamics. This protein interacts with components of the mitochondrial contact site and cristae organizing system (MICOS) complex which is important for maintaining cristae structure. The protein's role in energy production underlines its importance especially in tissues with high energy demand. By stabilizing mitochondrial architecture TMEM65 facilitates effective oxidative phosphorylation supporting ATP synthesis.

Pathways

TMEM65 significantly impacts the oxidative phosphorylation and electron transport chain pathways. It plays a role by interacting with proteins such as Nicotinamide Adenine Dinucleotide (NADH) dehydrogenase. This interaction helps in electron transport essential for generating the proton gradient used to drive ATP synthesis a fundamental cellular process to provide energy.

TMEM65 mutations associate with cardiomyopathy and neurodegenerative diseases. In cardiomyopathy faulty TMEM65 disrupts normal mitochondrial function affecting heart muscle and leading to impaired cardiac contractility. TMEM65's role here connects it to proteins like ATP synthase further influencing its involvement in energy metabolism disorders. Understanding these interactions could offer insights into potential therapeutic approaches targeting mitochondrial function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for maintaining proper cardiac intercalated disk (ICD) structure and function as well as cardiac conduction velocity in the heart. Its association with SCN1B is required for stabilizing the perinexus in the ICD and for localization of GJA1 and SCN5A to the ICD. May regulate the function of the gap junction protein GJA1 and may contribute to the stability and proper localization of GJA1 to cardiac intercalated disk thereby regulating gap junction communication (By similarity). May also play a role in the regulation of mitochondrial respiration and mitochondrial DNA copy number maintenance (PubMed : 28295037).
See full target information TMEM65
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 43:931-943 PubMed38341472

2024

TMEM65 promotes gastric tumorigenesis by targeting YWHAZ to activate PI3K-Akt-mTOR pathway and is a therapeutic target.

Applications

Unspecified application

Species

Unspecified reactive species

Lingxue Shi,Xiaohong Wang,Shang Guo,Hongyan Gou,Haiyun Shang,Xiaojia Jiang,Chunxian Wei,Jia Wang,Chao Li,Lihong Wang,Zengren Zhao,Weifang Yu,Jun Yu

Experimental dermatology 31:1253-1263 PubMed35514241

2022

Silencing of Rab23 by siRNA inhibits ultraviolet B-induced melanogenesis via downregulation of PKA/CREB/MITF.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanjin Wei,Min Huang,Yi Yang,Yali Liu,Sumin Chi,Chengxin Li
View all publications
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