Rabbit Polyclonal TMEM66/SARAF antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SARAF aa 1-250.
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- Cellular physiology and biochemistry : internation 36:1101-152015Cigarette Smoke and Estrogen Signaling in Human Airway Smooth Muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVenkatachalem Sathish et. al.PubMed 26113109
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SARAF aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q96BY9
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Target data
Function
Negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions.
Alternative names
TMEM66, XTP3, HSPC035, NPD003, PSEC0019, UNQ1967/PRO4499, SARAF, Store-operated calcium entry-associated regulatory factor, SOCE-associated regulatory factor, HBV X-transactivated gene 3 protein, HBV XAg-transactivated protein 3, Protein FOAP-7, Transmembrane protein 66
Recommended products
Rabbit Polyclonal TMEM66/SARAF antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SARAF aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SARAF aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q96BY9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TMEM66 also known as SARAF acts as a negative regulator of store-operated calcium entry (SOCE). It plays a role in maintaining cellular calcium homeostasis by limiting calcium influx. TMEM66 has a mass of approximately 36 kDa and is encoded by the SARAF gene. It is primarily expressed in the endoplasmic reticulum membrane but can also localize to the plasma membrane depending on the cellular context. It mainly appears in tissues with high metabolic or excitatory activity like the brain and heart.
- Biological function summary
TMEM66/SARAF functions to prevent calcium overload in cells by interacting with the calcium release-activated calcium (CRAC) channel components. It acts as a part of the larger store-operated calcium channel complex which includes STIM1 and Orai1. The interaction of TMEM66 with these proteins modulates their activity and ensures a controlled calcium entry into the cell playing a protective role during stimulations that otherwise might cause cellular damage due to excessive calcium levels.
- Pathways
TMEM66/SARAF directly influences the calcium signaling pathway and is also indirectly involved in the MAPK/ERK pathway. Calcium signaling is essential for various cellular processes including muscle contraction neurotransmitter release and gene expression. TMEM66 regulates this pathway by modulating Orai1 activity which is a critical component of the calcium influx mechanism. The MAPK/ERK pathway associated with cell proliferation and differentiation also interacts with calcium levels bridging a connection to TMEM66's role in cellular responses.
- Associated diseases and disorders
Research links TMEM66/SARAF to cardiac hypertrophy and neurodegenerative diseases. Abnormalities in calcium homeostasis influenced by TMEM66 can lead to pathologies like excess growth of heart muscle cells (cardiac hypertrophy). Additionally disrupted calcium signaling related to TMEM66's dysregulation potentially contributes to neuronal damage seen in neurodegenerative conditions. The protein's interaction with STIM1 and Orai1 underlies these disease connections as disturbances in these proteins' functions can disrupt normal calcium homeostasis potentially leading to cell injury or death.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-TMEM66/SARAF antibody (ab154538)
All lanes: Western blot - Anti-TMEM66/SARAF antibody (ab154538) at 1/1000 dilution
All lanes: Jurkat whole cell lysate at 30 µg
Predicted band size: 37 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM66/SARAF antibody (ab154538)
Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling TMEM66/SARAF with ab154538 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TMEM66/SARAF antibody (ab154538)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling TMEM66/SARAF with ab154538 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
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