JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB154538

Anti-TMEM66/SARAF antibody

4

(1 Review)

|

(1 Publication)

Rabbit Polyclonal TMEM66/SARAF antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SARAF aa 1-250.

View Alternative Names

TMEM66, XTP3, HSPC035, NPD003, PSEC0019, UNQ1967/PRO4499, SARAF, Store-operated calcium entry-associated regulatory factor, SOCE-associated regulatory factor, HBV X-transactivated gene 3 protein, HBV XAg-transactivated protein 3, Protein FOAP-7, Transmembrane protein 66

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM66/SARAF antibody (AB154538)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM66/SARAF antibody (AB154538)

Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling TMEM66/SARAF with ab154538 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-TMEM66/SARAF antibody (AB154538)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TMEM66/SARAF antibody (AB154538)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling TMEM66/SARAF with ab154538 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

Western blot - Anti-TMEM66/SARAF antibody (AB154538)
  • WB

Unknown

Western blot - Anti-TMEM66/SARAF antibody (AB154538)

All lanes:

Western blot - Anti-TMEM66/SARAF antibody (ab154538) at 1/1000 dilution

All lanes:

Jurkat whole cell lysate at 30 µg

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SARAF aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q96BY9

style
left-column

Complementary Products

Reagents, Controls & Accessories

AB171524

TMB ELISA Substrate (Fast Kinetic Rate)

ELISA - TMB ELISA Substrate (Fast Kinetic Rate) (AB171524)

  • 0
  • 0
Reagents, Controls & Accessories

AB210902

TMB substrate 1X

  • 0
  • 0
Primary Antibodies

AB76020

Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (AB76020)

  • 5
  • 18
Primary Antibodies

AB131255

Anti-GBP1 antibody [EPR8285]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBP1 antibody [EPR8285] (AB131255)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM66 also known as SARAF acts as a negative regulator of store-operated calcium entry (SOCE). It plays a role in maintaining cellular calcium homeostasis by limiting calcium influx. TMEM66 has a mass of approximately 36 kDa and is encoded by the SARAF gene. It is primarily expressed in the endoplasmic reticulum membrane but can also localize to the plasma membrane depending on the cellular context. It mainly appears in tissues with high metabolic or excitatory activity like the brain and heart.
Biological function summary

TMEM66/SARAF functions to prevent calcium overload in cells by interacting with the calcium release-activated calcium (CRAC) channel components. It acts as a part of the larger store-operated calcium channel complex which includes STIM1 and Orai1. The interaction of TMEM66 with these proteins modulates their activity and ensures a controlled calcium entry into the cell playing a protective role during stimulations that otherwise might cause cellular damage due to excessive calcium levels.

Pathways

TMEM66/SARAF directly influences the calcium signaling pathway and is also indirectly involved in the MAPK/ERK pathway. Calcium signaling is essential for various cellular processes including muscle contraction neurotransmitter release and gene expression. TMEM66 regulates this pathway by modulating Orai1 activity which is a critical component of the calcium influx mechanism. The MAPK/ERK pathway associated with cell proliferation and differentiation also interacts with calcium levels bridging a connection to TMEM66's role in cellular responses.

Research links TMEM66/SARAF to cardiac hypertrophy and neurodegenerative diseases. Abnormalities in calcium homeostasis influenced by TMEM66 can lead to pathologies like excess growth of heart muscle cells (cardiac hypertrophy). Additionally disrupted calcium signaling related to TMEM66's dysregulation potentially contributes to neuronal damage seen in neurodegenerative conditions. The protein's interaction with STIM1 and Orai1 underlies these disease connections as disturbances in these proteins' functions can disrupt normal calcium homeostasis potentially leading to cell injury or death.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity : possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions.
See full target information SARAF
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular physiology and biochemistry : internation 36:1101-15 PubMed26113109

2015

Cigarette Smoke and Estrogen Signaling in Human Airway Smooth Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Venkatachalem Sathish,Michelle R Freeman,Emma Long,Michael A Thompson,Christina M Pabelick,Y S Prakash
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com