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AB250985

Anti-TMEM9B antibody [EPR14339] - BSA and Azide free

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Rabbit Recombinant Monoclonal TMEM9B antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

C11orf15, UNQ712/PRO1375, TMEM9B, Transmembrane protein 9B

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TMEM9B antibody [EPR14339] - BSA and Azide free (AB250985)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TMEM9B antibody [EPR14339] - BSA and Azide free (AB250985)

This data was developed using ab189253, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling TMEM9B with ab189253 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Western blot - Anti-TMEM9B antibody [EPR14339] - BSA and Azide free (AB250985)
  • WB

Supplier Data

Western blot - Anti-TMEM9B antibody [EPR14339] - BSA and Azide free (AB250985)

This data was developed using ab189253, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TMEM9B antibody [EPR14339] (<a href='/en-us/products/primary-antibodies/tmem9b-antibody-epr14339-ab189253'>ab189253</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

false

Western blot - Anti-TMEM9B antibody [EPR14339] - BSA and Azide free (AB250985)
  • WB

Supplier Data

Western blot - Anti-TMEM9B antibody [EPR14339] - BSA and Azide free (AB250985)

This data was developed using ab189253, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TMEM9B antibody [EPR14339] (<a href='/en-us/products/primary-antibodies/tmem9b-antibody-epr14339-ab189253'>ab189253</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

BxPC-3 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14339

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250985 is the carrier-free version of ab189253.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM9B also known as Transmembrane Protein 9B is a protein involved in cellular processes like signal transduction and membrane trafficking. The protein has a molecular mass of approximately 28.5 kDa. TMEM9B expresses in various tissues including liver and spleen suggesting its role in multiple cellular functions. It localizes primarily to the endosomal and lysosomal membranes where it participates in cellular homeostasis and signaling pathways.
Biological function summary

This protein is involved in several cell functions beyond simple signal transduction. TMEM9B mediates immune responses and regulates inflammatory processes. It does not act in isolation; instead it forms part of larger protein complexes that influence endosomal maturation and acidification therefore enabling effective lysosomal function. This regulation is important for maintaining cellular integrity through proper endocytosis and degradation of cellular debris.

Pathways

TMEM9B plays a significant role within the NF-kB signaling pathway and Toll-like receptor pathways. In the NF-kB pathway TMEM9B interacts with proteins like IKK complexes influencing inflammatory responses at a cellular level. In the Toll-like receptor pathway its interaction with adaptor molecules such as MyD88 aids in initiating immune defense mechanisms. These interactions allow TMEM9B to serve as an important regulator in maintaining cellular and systemic inflammatory balance.

TMEM9B shows association with conditions like rheumatoid arthritis and systemic lupus erythematosus. These associations arise due to its regulation of inflammation and immune responses. The protein’s involvement in these conditions reveals an interaction with other key regulators like TNF-alpha and interleukin-6 which are pivotal in inflammatory diseases. Understanding TMEM9B involvement in such disorders may provide novel therapeutic targets for managing chronic inflammatory conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Enhances production of pro-inflammatory cytokines induced by TNF, IL1B, and TLR ligands. Has a role in TNF activation of both the NF-kappaB and MAPK pathways.
See full target information TMEM9B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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