Rabbit Polyclonal TMEPAI antibody. Suitable for IHC-P, ELISA, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PMEPA1.
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Publications
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- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:4365-43752018Prostate transmembrane protein androgen induced 1 is induced by activation of osteoclasts and regulates bone resorption.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianghe Xu,Hirohito Hirata,Makoto Shiraki,Asana Kamohara,Kenichi Nishioka,Hiroshi Miyamoto,Toshio Kukita,Akiko KukitaPubMed 30557043
- Gastroenterology 148:1427-37.e82015Transforming Growth Factor β Signaling in Colorectal Cancer Cells With Microsatellite Instability Despite Biallelic Mutations in TGFBR2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNoel F C C de Miranda et. al.PubMed 25736321
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PMEPA1. The exact immunogen used to generate this antibody is proprietary information. Database link Q969W9
Reactivity data
Select an application| IHC-P | ELISA | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
Target data
Function
Functions as a negative regulator of TGF-beta signaling and thereby probably plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production and immunosuppression. In the canonical TGF-beta pathway, ZFYVE9/SARA recruits the intracellular signal transducer and transcriptional modulators SMAD2 and SMAD3 to the TGF-beta receptor. Phosphorylated by the receptor, SMAD2 and SMAD3 then form a heteromeric complex with SMAD4 that translocates to the nucleus to regulate transcription. Through interaction with SMAD2 and SMAD3, LDLRAD4 may compete with ZFYVE9 and SMAD4 and prevent propagation of the intracellular signal (PubMed:20129061, PubMed:24627487). Also involved in down-regulation of the androgen receptor (AR), enhancing ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4 (PubMed:18703514).
Alternative names
STAG1, TMEPAI, PMEPA1, Protein TMEPAI, Prostate transmembrane protein androgen induced 1, Solid tumor-associated 1 protein, Transmembrane prostate androgen-induced protein
Recommended products
Rabbit Polyclonal TMEPAI antibody. Suitable for IHC-P, ELISA, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PMEPA1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PMEPA1. The exact immunogen used to generate this antibody is proprietary information. Database link Q969W9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TMEPAI also known as TMPRSS6-associated product containing transcript is a transmembrane protein. It has a molecular weight of approximately 26 kDa. TMEPAI is widely expressed in various tissues with particularly high expression in the lungs prostate and breast. It interacts with the cytoplasmic signaling proteins through its PY motifs modulating their activities directly. TMEPAI plays an important role in cellular pathways affecting cellular functions and interactions.
- Biological function summary
This protein modulates signaling pathways and cell growth regulation. It is well known for its involvement in the TGF-beta (Transforming Growth Factor-beta) signaling pathway. TMEPAI acts as a negative regulator inhibiting TGF-beta's tumor-suppressive functions through interaction with Smad proteins. While it does not form part of a complex itself it significantly influences the functional dynamics of the TGF-beta pathway constituents impacting cellular proliferation and survival.
- Pathways
TMEPAI exerts its functional significance primarily within the TGF-beta and PI3K/Akt signaling pathways. In the TGF-beta pathway TMEPAI modulates the signaling cascade by interacting with Smad2/3 and Smad4. The regulation of these proteins by TMEPAI suppresses TGF-beta-induced growth inhibition. In the PI3K/Akt pathway TMEPAI influences cell survival and proliferation indirectly through these interactions positioning it as an important modulator in these pathways.
- Associated diseases and disorders
TMEPAI has significant implications in cancer particularly in breast and prostate cancer. It facilitates cancer progression by overriding the tumor-suppressive effects of TGF-beta signaling. The interactions with Smad proteins like Smad2/3 highlight its role in oncogenic pathways. Moreover in prostate cancer the dysregulation of TMEPAI links with increased aggressive behavior of cancer cells exacerbating disease progression. Understanding these interactions provides insights into potential targets for therapeutic intervention in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-TMEPAI antibody (ab128006)
All lanes: Western blot - Anti-TMEPAI antibody (ab128006) at 1/100 dilution
Lane 1: HT29 cell extract
Lane 2: HT29 cell extract with synthesized peptide
Predicted band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEPAI antibody (ab128006)
ab128006, at a concentration of 10 µg/ml, staining TMEPAI in paraffin-embedded Human Prostate, Smooth Muscle tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-TMEPAI antibody (ab128006)
ab128006, at a dilution of 1/100, staining TMEPAI in HuvEc cells by Immunofluorescence. The picture on the right is treated with the synthesized peptide.
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