JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB151702

Anti-TMF antibody [EPR10671(B)]

5

(3 Reviews)

|

(2 Publications)

Rabbit Recombinant Monoclonal TMF antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

ARA160, TMF1, TATA element modulatory factor, TMF, Androgen receptor coactivator 160 kDa protein, Androgen receptor-associated protein of 160 kDa

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TMF antibody [EPR10671(B)] (AB151702)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TMF antibody [EPR10671(B)] (AB151702)

Immunofluorescent analysis of MCF7 cells labeling TMF with ab151702 at 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-TMF antibody [EPR10671(B)] (AB151702)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TMF antibody [EPR10671(B)] (AB151702)

Intracellular flow cytometric analysis of permeabilized HT1376 cells labeling TMF with ab151702 at 1/10 dilution (red). Rabbit IgG negative control (green).

Western blot - Anti-TMF antibody [EPR10671(B)] (AB151702)
  • WB

Unknown

Western blot - Anti-TMF antibody [EPR10671(B)] (AB151702)

All lanes:

Western blot - Anti-TMF antibody [EPR10671(B)] (ab151702) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HT1376 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 123 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10671(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transcriptional mediator factor (TMF) also known as TMF/ARA160 weighs around 160 kDa. It resides in the nucleus and cytoplasm of cells. TMF is a multifunctional protein and plays a part in transcription regulation. It interacts with protein complexes influencing their stability and function. In addition to its nuclear roles TMF associates with the Golgi apparatus suggesting its diverse function in different cellular contexts.
Biological function summary

The role of TMF extends to various cellular processes including transcriptional regulation signal transduction and vesicle transport. It forms part of larger protein complexes which modulate gene expression and cellular signaling networks. TMF serves as a scaffold facilitating the interactions between transcription factors and other regulatory proteins. This makes TMF an active regulator of transcriptional control impacting gene expression patterns.

Pathways

Many known cellular pathways require TMF's involvement. TMF impacts the NF-kappaB signaling pathway and the Wnt signaling pathway. It directly interacts with proteins like RelA in the NF-kappaB pathway where it modulates transcriptional activation. TMF's influence on these pathways highlights its significant role in regulating cellular responses and maintaining cellular homeostasis.

Research shows that TMF has associations with cancer and neurodegenerative diseases. Its abnormal expression or mutation links to tumorigenesis particularly in cancers such as prostate and breast cancer. Its interaction with proteins such as beta-catenin in the Wnt pathway associates TMF with oncogenic processes. Moreover TMF's role in the Golgi apparatus links it to neurodegenerative disorders where disruptions in vesicle transport are common features.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Potential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes : one from endosomes to the Golgi and the other from the Golgi to the ER. This protein binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP).
See full target information TMF1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:29769 PubMed39616227

2024

Kaempferol alleviates rheumatoid arthritis through pyroptosis based on bioinformatics analysis and experimental validation.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Ling,Nina Ren,Yuzheng Yang,Hui Xu,Hong Xiong,Qiuyi Wang,Daomin Lu,Xueming Yao,Wukai Ma

Communications biology 4:1370 PubMed34876695

2021

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xihua Yue,Neeraj Tiwari,Lianhui Zhu,Hai Dang Truong Ngo,Jae-Min Lim,Bopil Gim,Shuaiyang Jing,Yijing Wang,Yi Qian,Intaek Lee
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB249125

Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9927] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9927] - BSA and Azide free (AB249125)

  • 0
  • 0
Primary Antibodies

AB154170

Anti-USP5 antibody [EPR10454]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-USP5 antibody [EPR10454] (AB154170)

  • 0
  • 0
Primary Antibodies

AB32136

Anti-Amyloid Precursor Protein antibody [Y188]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Precursor Protein antibody [Y188] (AB32136)

  • 4
  • 9
Primary Antibodies

AB182564

Anti-POLD3 antibody [EP9480]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-POLD3 antibody [EP9480] (AB182564)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com