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AB249024

Anti-TMF antibody [EPR10671(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TMF antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ARA160, TMF1, TATA element modulatory factor, TMF, Androgen receptor coactivator 160 kDa protein, Androgen receptor-associated protein of 160 kDa

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TMF antibody [EPR10671(B)] - BSA and Azide free (AB249024)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TMF antibody [EPR10671(B)] - BSA and Azide free (AB249024)

This data was developed using ab151702, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of MCF7 cells labeling TMF with ab151702 at 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-TMF antibody [EPR10671(B)] - BSA and Azide free (AB249024)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TMF antibody [EPR10671(B)] - BSA and Azide free (AB249024)

This data was developed using ab151702, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HT1376 cells labeling TMF with ab151702 at 1/10 dilution (red). Rabbit IgG negative control (green).

Western blot - Anti-TMF antibody [EPR10671(B)] - BSA and Azide free (AB249024)
  • WB

Unknown

Western blot - Anti-TMF antibody [EPR10671(B)] - BSA and Azide free (AB249024)

This data was developed using ab151702, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TMF antibody [EPR10671(B)] (<a href='/en-us/products/primary-antibodies/tmf-antibody-epr10671b-ab151702'>ab151702</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HT1376 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 123 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10671(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab249024 is the carrier-free version of ab151702.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transcriptional mediator factor (TMF) also known as TMF/ARA160 weighs around 160 kDa. It resides in the nucleus and cytoplasm of cells. TMF is a multifunctional protein and plays a part in transcription regulation. It interacts with protein complexes influencing their stability and function. In addition to its nuclear roles TMF associates with the Golgi apparatus suggesting its diverse function in different cellular contexts.
Biological function summary

The role of TMF extends to various cellular processes including transcriptional regulation signal transduction and vesicle transport. It forms part of larger protein complexes which modulate gene expression and cellular signaling networks. TMF serves as a scaffold facilitating the interactions between transcription factors and other regulatory proteins. This makes TMF an active regulator of transcriptional control impacting gene expression patterns.

Pathways

Many known cellular pathways require TMF's involvement. TMF impacts the NF-kappaB signaling pathway and the Wnt signaling pathway. It directly interacts with proteins like RelA in the NF-kappaB pathway where it modulates transcriptional activation. TMF's influence on these pathways highlights its significant role in regulating cellular responses and maintaining cellular homeostasis.

Research shows that TMF has associations with cancer and neurodegenerative diseases. Its abnormal expression or mutation links to tumorigenesis particularly in cancers such as prostate and breast cancer. Its interaction with proteins such as beta-catenin in the Wnt pathway associates TMF with oncogenic processes. Moreover TMF's role in the Golgi apparatus links it to neurodegenerative disorders where disruptions in vesicle transport are common features.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Potential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes : one from endosomes to the Golgi and the other from the Golgi to the ER. This protein binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP).
See full target information TMF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 60:2744-2760.e9 PubMed40460835

2025

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano De Tito,Eugenia Almacellas,Daniel Dai Yu,Emily Millard,Wenxin Zhang,Cecilia de Heus,Christophe Queval,Javier H Hervás,Enrica Pellegrino,Ioanna Panagi,Ditte Fogde,Teresa L M Thurston,Judith Klumperman,Maximiliano Gutierrez,Sharon A Tooze
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com