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AB220232

Anti-TMLHE antibody

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(1 Publication)

Rabbit Polyclonal TMLHE antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TMLHE aa 300 to C-terminus.

View Alternative Names

TMLH, TMLHE, Epsilon-trimethyllysine 2-oxoglutarate dioxygenase, Epsilon-trimethyllysine hydroxylase, TML hydroxylase, TML-alpha-ketoglutarate dioxygenase, TML dioxygenase, TMLD

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-TMLHE antibody (AB220232)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TMLHE antibody (AB220232)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized HEK 293 cells labeling TMHLE with ab220232 at 4 μg/ml (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TMLHE aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NVH6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMLHE also known as trimethyllysine hydroxylase epsilon is an enzyme with a mass of approximately 43 kDa. It is expressed mainly in the kidneys liver and heart. This enzyme catalyzes the hydroxylation of trimethyllysine to hydroxytrimethyllysine a critical step in the carnitine biosynthesis pathway. TMLHE performs its function as a dioxygenase using Fe(II) and 2-oxoglutarate as cofactors. It plays a significant role in the metabolism of fatty acids.
Biological function summary

The enzyme operates within the context of carnitine production which is necessary for the transport of long-chain fatty acids into mitochondria for beta-oxidation. TMLHE does not form part of a larger enzyme complex but functions independently. This biological role underpins energy production through fatty acids highlighting its importance in cellular metabolism. Its activity directly impacts carnitine availability and subsequently energy yield from lipid sources.

Pathways

TMLHE operates within the carnitine biosynthesis pathway significant for lipid metabolism. The enzyme shares functional connections with other enzymes in this pathway such as BBOX1 (gamma-butyrobetaine hydroxylase) which further catalyzes carnitine synthesis. The carnitine transport pathway closely linked to energy production relies on these enzymes. TMLHE's role maintains flux through this pathway ensuring efficient fatty acid metabolism and energy extraction.

Mutations or malfunctions in TMLHE correlate with disorders like epilepsy and autism spectrum disorders. Disturbances in TMLHE function can lead to altered carnitine levels resulting in impaired brain metabolism. This impairment sometimes overlaps with deficiencies in related enzymes such as CPT1A (carnitine palmitoyltransferase I) which also impact fatty acid transportation. Understanding TMLHE's role aids in uncovering the metabolic contributions to these neurological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts trimethyllysine (TML) into hydroxytrimethyllysine (HTML) (PubMed : 11431483, PubMed : 23092983).
See full target information TMLHE
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 25:105620 PubMed36465115

2022

TWIK-related acid-sensitive K channel 2 promotes renal fibrosis by inducing cell-cycle arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhang,Jing Chen,Yufei Lu,Yan Yang,Weize Chen,Bo Shen,Jiachang Hu,Ping Jia,Sujuan Xu,Yiqin Shi,Yichun Ning,Jialin Wang,Yi Fang,Shuan Zhao,Yang Li,Yan Dai,Xiaoyan Zhang,Meng Xiang,Yang Tian,Zhichao Liu,Nana Song,Xiaoqiang Ding
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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