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AB97842

Anti-TMLHE antibody

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(1 Publication)

Rabbit Polyclonal TMLHE antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TMLHE aa 100-400.

View Alternative Names

TMLH, TMLHE, Epsilon-trimethyllysine 2-oxoglutarate dioxygenase, Epsilon-trimethyllysine hydroxylase, TML hydroxylase, TML-alpha-ketoglutarate dioxygenase, TML dioxygenase, TMLD

1 Images
Western blot - Anti-TMLHE antibody (AB97842)
  • WB

Unknown

Western blot - Anti-TMLHE antibody (AB97842)

12% SDS PAGE

All lanes:

Western blot - Anti-TMLHE antibody (ab97842) at 1/200 dilution

All lanes:

HeLaS3 whole cell lysate at 30 µg

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human TMLHE aa 100-400. The exact immunogen used to generate this antibody is proprietary information.

Q9NVH6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMLHE also known as trimethyllysine hydroxylase epsilon is an enzyme with a mass of approximately 43 kDa. It is expressed mainly in the kidneys liver and heart. This enzyme catalyzes the hydroxylation of trimethyllysine to hydroxytrimethyllysine a critical step in the carnitine biosynthesis pathway. TMLHE performs its function as a dioxygenase using Fe(II) and 2-oxoglutarate as cofactors. It plays a significant role in the metabolism of fatty acids.
Biological function summary

The enzyme operates within the context of carnitine production which is necessary for the transport of long-chain fatty acids into mitochondria for beta-oxidation. TMLHE does not form part of a larger enzyme complex but functions independently. This biological role underpins energy production through fatty acids highlighting its importance in cellular metabolism. Its activity directly impacts carnitine availability and subsequently energy yield from lipid sources.

Pathways

TMLHE operates within the carnitine biosynthesis pathway significant for lipid metabolism. The enzyme shares functional connections with other enzymes in this pathway such as BBOX1 (gamma-butyrobetaine hydroxylase) which further catalyzes carnitine synthesis. The carnitine transport pathway closely linked to energy production relies on these enzymes. TMLHE's role maintains flux through this pathway ensuring efficient fatty acid metabolism and energy extraction.

Mutations or malfunctions in TMLHE correlate with disorders like epilepsy and autism spectrum disorders. Disturbances in TMLHE function can lead to altered carnitine levels resulting in impaired brain metabolism. This impairment sometimes overlaps with deficiencies in related enzymes such as CPT1A (carnitine palmitoyltransferase I) which also impact fatty acid transportation. Understanding TMLHE's role aids in uncovering the metabolic contributions to these neurological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts trimethyllysine (TML) into hydroxytrimethyllysine (HTML) (PubMed : 11431483, PubMed : 23092983).
See full target information TMLHE
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:13989 PubMed38886371

2024

In vitro evolution and whole genome analysis to study chemotherapy drug resistance in haploid human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Carlos Jado,Michelle Dow,Krypton Carolino,Adam Klie,Gregory J Fonseca,Trey Ideker,Hannah Carter,Elizabeth A Winzeler
View all publications
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Product promise

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