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AB222842

Anti-TMM64 antibody

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(1 Publication)

Rabbit Polyclonal TMM64 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Transmembrane protein 64 aa 1-150.

View Alternative Names

Transmembrane protein 64, TMEM64

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMM64 antibody (AB222842)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMM64 antibody (AB222842)

Paraffin-embedded human lung tissue stained for TMM64 using ab222842 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-TMM64 antibody (AB222842)
  • WB

Supplier Data

Western blot - Anti-TMM64 antibody (AB222842)

All lanes:

Western blot - Anti-TMM64 antibody (ab222842) at 1/1000 dilution

All lanes:

mouse brain tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG at 1/10000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human Transmembrane protein 64 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q6YI46

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMM64 also known as Transmembrane Protein 64 is a protein that interacts with various cellular mechanisms. It has a molecular weight of approximately 64 kDa. This protein localizes within both the cytoplasmic and membrane regions in several cell types. Most notably it shows higher expression in adipose tissues and certain layers of the skin indicating its role in specific physiological processes related to these tissues.
Biological function summary

TMM64 interacts with molecular complexes that regulate lipid metabolism and energy homeostasis. Within cells this protein contributes to the regulation of cellular energy status by modulating interactions between lipid droplets and mitochondria. It influences lipid storage and usage by affecting enzymes involved in lipid biosynthesis pathways. This suggests its involvement in energy balance and metabolic processes.

Pathways

TMM64 has significant roles in the lipid metabolism and insulin signaling pathways. Within these pathways it acts in conjunction with proteins such as AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptors (PPARs). In the lipid metabolism pathway TMM64 modulates the response to metabolic signals assisting in the adaptation of lipid storage and mobilization. It influences how cells react to insulin signals affecting glucose uptake and storage as lipids.

TMM64 appears significant in conditions like obesity and type 2 diabetes mellitus. Dysregulation of TMM64 expression or function correlates with increased lipid accumulation and impaired insulin action. This association relates TMM64 to insulin receptor substrate 1 (IRS1) and its role in insulin sensitivity. By influencing pathways critical to lipid and glucose homeostasis alterations in TMM64 may contribute to the development and progression of metabolic diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Positively regulates TNFSF11-induced osteoclast differentiation. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with SERCA2 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca (2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca (2+) signaling cascades that promote osteoclast differentiation and activation. Negatively regulates osteoblast differentiation and positively regulates adipocyte differentiation via modulation of the canonical Wnt signaling pathway. Mediates the switch in lineage commitment to osteogenesis rather than to adipogenesis in mesenchymal stem cells by negatively regulating the expression, activity and nuclear localization of CTNNB1.
See full target information Transmembrane protein 64
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 10: PubMed33491649

2021

TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando R Balestra,Andrés Domínguez-Calvo,Benita Wolf,Coralie Busso,Alizée Buff,Tessa Averink,Marita Lipsanen-Nyman,Pablo Huertas,Rosa M Ríos,Pierre Gönczy
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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