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AB280567

Anti-TMPRSS2 antibody [EPR24407-87]

  • 20ul selling size
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

0

(1 Review)

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(5 Publications)

Rabbit Recombinant Monoclonal TMPRSS2 antibody. Suitable for ICC/IF, Flow Cyt and reacts with Human samples. Cited in 5 publications.

View Alternative Names

PRSS10, TMPRSS2, Transmembrane protease serine 2, Serine protease 10

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TMPRSS2 antibody [EPR24407-87] (AB280567)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TMPRSS2 antibody [EPR24407-87] (AB280567)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LNCaP and HEK-293 cells labelling TMPRSS2 with ab280567 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in LNCaP cell line.

Negative control : HEK-293 (PMID : 20631123).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-TMPRSS2 antibody [EPR24407-87] (AB280567)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TMPRSS2 antibody [EPR24407-87] (AB280567)

ab280567 staining TMPRSS2 in wild-type LnCAP cells (top panel) and TMPRSS2 knockout LnCAP cells (ab273745) (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab280567 at 1μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Flow Cytometry - Anti-TMPRSS2 antibody [EPR24407-87] (AB280567)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TMPRSS2 antibody [EPR24407-87] (AB280567)

Flow cytometric analysis of HEK-293T (Human embryonic kidney epithelial cell, Left)/ LNCaP (Human prostate carcinoma epithelial cell, Right) cells labelling TMPRSS2 with ab280567 at 1/500 dilution (0.1μg)/ red compared with Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

Negative control : HEK-239T (PMID : 20631123).

Gated on viable cells.

  • 421 Alexa Fluor® 405

    Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed

  • 665 Alexa Fluor® 647

    Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed

  • Biotin

    Goat Anti-Rabbit IgG H&L (Biotin)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24407-87

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plasma membrane-anchored serine protease that cleaves at arginine residues (PubMed : 32703818, PubMed : 35676539, PubMed : 37990007, PubMed : 38964328). Participates in proteolytic cascades of relevance for the normal physiologic function of the prostate (PubMed : 25122198). Androgen-induced TMPRSS2 activates several substrates that include pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14 leading to extracellular matrix disruption and metastasis of prostate cancer cells (PubMed : 15537383, PubMed : 25122198, PubMed : 26018085). In addition, activates trigeminal neurons and contribute to both spontaneous pain and mechanical allodynia (By similarity).. (Microbial infection) Facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via two independent mechanisms, proteolytic cleavage of ACE2 receptor which promotes viral uptake, and cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry (PubMed : 24227843, PubMed : 32142651, PubMed : 32404436, PubMed : 33051876, PubMed : 34159616, PubMed : 35676539, PubMed : 37990007). The cleavage of SARS-COV2 spike glycoprotein occurs between the S2 and S2' site (PubMed : 32703818). Upon SARS-CoV-2 infection, increases syncytia formation by accelerating the fusion process (PubMed : 33051876, PubMed : 34159616, PubMed : 35676539). Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9); involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.. (Microbial infection) Receptor for human coronavirus HKU1-CoV, acts synergistically with disialoside glycans to facilitate the entry of the virus. After binding to cell-surface disialoside glycans, the viral S protein interacts with the inactive form of TMPRSS2 and inhibits its protease activity.
See full target information TMPRSS2
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e70730 PubMed40990068

2025

PINK1 deficiency permits the development of Lewy body dementia with coexisting Aβ pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Tong-Yao Gao,Xu-Zheng Wang,Yu-Han Xie,Tong Wang,Yun-Bi Lu,Lu-Long Huang,Cong Chen,Ming Zhang,Xin Ma,Ya-Ling Chen,Fu-Xiang Liang,Zhi-Ling Lou,Jin-Sheng Li,Yi-Fan Yu,Jian-Bin Wu,Xiao-Ru Ma,Hua-Li Wang,Chun Tang,Wei-Ping Zhang

Fluids and barriers of the CNS 20:76 PubMed37875964

2023

SARS-CoV-2 infects epithelial cells of the blood-cerebrospinal fluid barrier rather than endothelial cells or pericytes of the blood-brain barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Stüdle,Hideaki Nishihara,Sven Wischnewski,Laila Kulsvehagen,Sylvain Perriot,Hiroshi Ishikawa,Horst Schroten,Stephan Frank,Nikolaus Deigendesch,Renaud Du Pasquier,Lucas Schirmer,Anne-Katrin Pröbstel,Britta Engelhardt

Scientific reports 13:11783 PubMed37479788

2023

Identification and validation of fusidic acid and flufenamic acid as inhibitors of SARS-CoV-2 replication using DrugSolver CavitomiX.

Applications

Unspecified application

Species

Unspecified reactive species

M Hetmann,C Langner,V Durmaz,M Cespugli,K Köchl,A Krassnigg,K Blaschitz,S Groiss,M Loibner,D Ruau,K Zatloukal,K Gruber,G Steinkellner,C C Gruber

PLoS pathogens 19:e1011131 PubMed36701392

2023

Targeting RNA G-quadruplex with repurposed drugs blocks SARS-CoV-2 entry.

Applications

Unspecified application

Species

Unspecified reactive species

Qiyu Tong,Geng Liu,Xiongbo Sang,Xinyue Zhu,Xiaoli Fu,Chao Dou,Yue Jian,Jiani Zhang,Sailan Zou,Guixiang Zhang,Xiao Du,Dan Liu,Shiqian Qi,Wei Cheng,Yan Tian,Xianghui Fu

The Journal of biological chemistry 298:102643 PubMed36309092

2022

Transmembrane serine protease TMPRSS2 implicated in SARS-CoV-2 infection is autoactivated intracellularly and requires N-glycosylation for regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yikai Zhang,Shijin Sun,Chunyu Du,Kaixuan Hu,Ce Zhang,Meng Liu,Qingyu Wu,Ningzheng Dong
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com