Anti-TMPRSS3 antibody [EPR7048] - BSA and Azide free (ab249403)

Rabbit Recombinant Monoclonal TMPRSS3 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] - BSA and Azide free (AB249403), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF	IP	WB
Human	Tested	Tested	Not recommended	Tested
Rat	Expected	Expected	Not recommended	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Target data

See full target information TMPRSS3

Function

Probable serine protease that plays a role in hearing. Acts as a permissive factor for cochlear hair cell survival and activation at the onset of hearing and is required for saccular hair cell survival (By similarity). Activates ENaC (in vitro).

Alternative names

ECHOS1, TADG12, UNQ323/PRO382, TMPRSS3, Transmembrane protease serine 3, Serine protease TADG-12, Tumor-associated differentially-expressed gene 12 protein

Recommended products

Rabbit Recombinant Monoclonal TMPRSS3 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR7048
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab249403 is the carrier-free version of Anti-TMPRSS3 antibody [EPR7048] ab167160.

Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] - BSA and Azide free (ab249403)
This data was developed using Anti-TMPRSS3 antibody [EPR7048] ab167160, the same antibody clone in a different buffer formulation.Anti-TMPRSS3 antibody [EPR7048] ab167160 showing +ve staining in Human cervical carcinoma. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] - BSA and Azide free (ab249403)
This data was developed using Anti-TMPRSS3 antibody [EPR7048] ab167160, the same antibody clone in a different buffer formulation.Anti-TMPRSS3 antibody [EPR7048] ab167160 showing +ve staining in Human normal tonsil. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] - BSA and Azide free (ab249403)
This data was developed using Anti-TMPRSS3 antibody [EPR7048] ab167160, the same antibody clone in a different buffer formulation.Anti-TMPRSS3 antibody [EPR7048] ab167160 showing +ve staining in Human lung adenocarcinoma. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] - BSA and Azide free (ab249403)
This data was developed using Anti-TMPRSS3 antibody [EPR7048] ab167160, the same antibody clone in a different buffer formulation.Anti-TMPRSS3 antibody [EPR7048] ab167160 showing +ve staining in Human normal kidney. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] - BSA and Azide free (ab249403)
This data was developed using Anti-TMPRSS3 antibody [EPR7048] ab167160, the same antibody clone in a different buffer formulation.Anti-TMPRSS3 antibody [EPR7048] ab167160 showing +ve staining in Human normal colon. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] - BSA and Azide free (ab249403)
This data was developed using Anti-TMPRSS3 antibody [EPR7048] ab167160, the same antibody clone in a different buffer formulation.Anti-TMPRSS3 antibody [EPR7048] ab167160 showing +ve staining in Human breast carcinoma. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-TMPRSS3 antibody [EPR7048] - BSA and Azide free (ab249403)
This data was developed using Anti-TMPRSS3 antibody [EPR7048] ab167160, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-TMPRSS3 antibody [EPR7048] (Anti-TMPRSS3 antibody [EPR7048] ab167160) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: PC-12 cell lysate at 10 µg
Lane 3: A431 cell lysate at 10 µg
Predicted band size: 47 kDa, 49 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] - BSA and Azide free (ab249403)
This data was developed using Anti-TMPRSS3 antibody [EPR7048] ab167160, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling TMPRSS3 with Anti-TMPRSS3 antibody [EPR7048] ab167160 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TMPRSS3 antibody [EPR7048] - BSA and Azide free (ab249403)
This data was developed using Anti-TMPRSS3 antibody [EPR7048] ab167160, the same antibody clone in a different buffer formulation.Immunocytochemical analysis of A431 cells labeling TMPRSS3 with Anti-TMPRSS3 antibody [EPR7048] ab167160 at a 1/250 dilution.