AB283684

Anti-TMS1/ASC antibody [EPR23978-28]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
KO Validated
What is this?

Be the first to review this product! Submit a review

(47 Publications)

Rabbit Recombinant Monoclonal TMS1/ASC antibody. Suitable for IP, Flow Cyt (Intra), WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 47 publications.

View Alternative Names

ASC, CARD5, TMS1, PYCARD, Apoptosis-associated speck-like protein containing a CARD, hASC, Caspase recruitment domain-containing protein 5, PYD and CARD domain-containing protein, Target of methylation-induced silencing 1

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 cells labelling TMS1/ASC with ab283684 at 1/100 (4.94 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic taining in THP-1 cell line. Negative control : HeLa (PMID : 11103776). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Flow Cytometry (Intracellular) - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell, Left) / THP-1 (Human monocytic leukemia monocyte, Right) cells labelling TMS1/ASC with ab283684 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control : HeLa (PMID : 11103776).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labelling TMS1/ASC with ab283684 at 1/2000 (0.247 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon cancer (PMID : 14643031). The section was incubated with ab283684 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling TMS1/ASC with ab283684 at 1/2000 (0.247 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil. The section was incubated with ab283684 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TMS1/ASC with ab283684 at 1/2000 (0.247 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon. The section was incubated with ab283684 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Supplier Data

Immunoprecipitation - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

TMS1/ASC was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate 20 ug with ab283684 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283684 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : THP-1 (human monocytic leukemia monocyte) whole cell lysate 20 ug

Lane 2 : ab283684 IP in THP-1 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab283684 in THP-1 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-TMS1/ASC antibody [EPR23978-28] (ab283684)

Predicted band size: 22 kDa

Observed band size: 12 kDa,17 kDa,24 kDa

false

Lab

Western blot - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

Western blot : Rabbit Monoclonal [EPR23978-28] to TMS1/ASC ab283684 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin (ab7291) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 22 kDa in Wild-type A549 cell lysates with no signal observed at this size in PYCARD knockout A549 cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-TMS1/ASC antibody [EPR23978-28] (ab283684) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

PYCARD knockout A549 at 20 µg

Lane 3:

HT-29 at 20 µg

Lane 4:

HEK-293 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Lab

Western blot - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

ASC has four isoforms. 24-kDa ASC, 17-kDa ASC-b and 12-kDa ASC-C are observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 20482797).

Exposure time : 81 seconds

All lanes:

Western blot - Anti-TMS1/ASC antibody [EPR23978-28] (ab283684) at 1/1000 dilution

Lane 1:

Human spleen tissue lysate at 20 µg

Lane 2:

Human colon tissue lysate at 20 µg

Lane 3:

Human tonsil tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 22 kDa

Observed band size: 12 kDa,17 kDa,24 kDa

false

Carrier free

Anti-TMS1/ASC antibody [EPR23978-28] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-TMS1/ASC antibody [EPR23978-28]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23978-28

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Functions as a key mediator in apoptosis and inflammation (PubMed : 11103777, PubMed : 12646168, PubMed : 15030775, PubMed : 17349957, PubMed : 17599095, PubMed : 19158675, PubMed : 19158676, PubMed : 19234215, PubMed : 19494289, PubMed : 21487011, PubMed : 24630722, PubMed : 25847972, PubMed : 30674671, PubMed : 34678144, PubMed : 36050480). Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner (PubMed : 11103777, PubMed : 12646168). Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3 (PubMed : 14730312, PubMed : 16964285). Involved in innate immune response by acting as an integral adapter in the assembly of various inflammasomes (NLRP1, NLRP2, NLRP3, NLRP6, AIM2 and probably IFI16) which recruit and activate caspase-1 leading to processing and secretion of pro-inflammatory cytokines (PubMed : 15030775, PubMed : 16982856, PubMed : 17349957, PubMed : 17599095, PubMed : 19158675, PubMed : 19158676, PubMed : 19234215, PubMed : 21487011, PubMed : 23530044, PubMed : 24630722, PubMed : 25847972, PubMed : 29440442, PubMed : 30674671, PubMed : 33980849, PubMed : 34678144, PubMed : 34706239). Caspase-1-dependent inflammation leads to macrophage pyroptosis, a form of cell death (PubMed : 24630722). The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions (PubMed : 14499617, PubMed : 19234215, PubMed : 24630722). Clustered PYCARD nucleates the formation of caspase-1 filaments through the interaction of their respective CARD domains, acting as a platform for of caspase-1 polymerization (PubMed : 24630722). In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1 (PubMed : 17349957). In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation (PubMed : 16964285). May be involved in RIGI-triggered pro-inflammatory responses and inflammasome activation (PubMed : 19915568). In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8 (PubMed : 19158675, PubMed : 19158676). In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2; the latter function is proposed to involve the nuclear form (PubMed : 22732093). Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome; this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways (PubMed : 12486103, PubMed : 16585594). For regulation of NF-kappa-B activating and inhibiting functions have been reported (PubMed : 12486103). Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK (PubMed : 12486103, PubMed : 16585594). Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing (PubMed : 16585594). Modulates host resistance to DNA virus infection, probably by inducing the cleavage of and inactivating CGAS in presence of cytoplasmic double-stranded DNA (PubMed : 28314590).. Isoform 2. May have a regulating effect on the function as inflammasome adapter.. Isoform 3. Seems to inhibit inflammasome-mediated maturation of interleukin-1 beta.

See full target information PYCARD

style

left-column

Publications (47)

Recent publications for all applications. Explore the full list and refine your search

Medical oncology (Northwood, London, England) 42:456 PubMed40883565

2025

Wedelolactone induces pyroptosis to suppress retinoblastoma by inhibiting the Nrf2/Keap1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhou Jiang,Hua Jiang,Guitao Wu,Ningdong Pang,Chuanqiang Niu,Zhouping Wang,Haibo Li

American journal of reproductive immunology (New York, N.Y. : 1989) 94:e70132 PubMed40815040

2025

ATP/P2X4 Regulates Inflammation and Oxidative Stress in Endometriosis Through NLRP3 Inflammasome-Dependent Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Wu,Yan Guo

Allergologia et immunopathologia 53:14-20 PubMed40682224

2025

Knockdown of ITIH4 reduces inflammatory damage and apoptosis of A549 cells induced by through NLRP3 inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhinan Zhang,Yixian Zhang,Bihe Zeng

European journal of medical research 30:586 PubMed40624560

2025

Vagus nerve stimulation: a promising strategy to combat pyroptosis and inflammation in traumatic brain injury through the OX-A/NLRP3/caspase-1/GSDMD signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Junwei Kang,Binkai Ren,Jia Wang,Yunliang Tang,Xiaoyang Dong

International journal of ophthalmology 18:792-801 PubMed40385128

2025

Fenofibrate mitigates the dysfunction of high glucose-driven human retinal microvascular endothelial cells by suppressing NLRP3 inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Shi,Hao-Min Chen,Ai-Hua Liu,Xiao-Rong Li

BMC gastroenterology 25:365 PubMed40360974

2025

Investigation of the mechanism by which miR-223-3p inhibits reflux esophagitis through targeting the NLRP3 inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Shuying Lin,Binbin Zheng,Ruchen Wu,Qiuli Wu,Xiangbo Chen

Scientific reports 15:16348 PubMed40348852

2025

TLR2 promotes the progression of diabetes mellitus with atherosclerosis via activating NLRP3 inflammasome and MyD88/NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sisi Chen,Min Xie,Yong Liu

Inflammation 48:3710-3725 PubMed40195181

2025

The Protective Effects of Vanillic Acid on LPS-induced Acute Lung Injury by Inhibiting STIM1-mediated NLRP3 Inflammasome Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Hai-Dong Li,Xia Sun,Jia-Hui Ni,Gui-Ze Feng,Xiao-Yan Shen,Hong-Bo Weng,Hao Fang

Cell biochemistry and function 43:e70064 PubMed40103178

2025

HDAC4-AS1/CTCF Transcriptionally Represses HDAC4 Under Stress, Whereas HDAC4 Inhibits Stress-Induced Syncytiotrophoblast Cellular Pyroptosis by Deacetylating NLRP3 and GSDMD.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Du,Qinghong Ji,Lihua Dong,Lanlan Wang,Gang Xin

Journal of nanobiotechnology 23:181 PubMed40050873

2025

Cerium-doped Prussian blue biomimetic nanozyme as an amplified pyroptosis inhibitor mitigate Aβ oligomer-induced neurotoxicity in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ma,Yu Tian,Chengzhong Du,Yang Zhu,Wen Huang,Chenyu Ding,Penghui Wei,Xuehan Yi,Zhangya Lin,Wenhua Fang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TMS1/ASC antibody [EPR23978-28]

Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

Flow Cytometry (Intracellular) - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

Immunoprecipitation - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

Western blot - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

Western blot - Anti-TMS1/ASC antibody [EPR23978-28] (AB283684)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (47)

Product promise