Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (ab307561)

Rabbit Recombinant Monoclonal TMS1/ASC antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (AB307561), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt (Intra)	ICC/IF	IHC-P	WB
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Tested	Tested
Rat	Expected	Not recommended	Not recommended	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

See full target information Pycard

Function

Functions as a key mediator in apoptosis and inflammation (PubMed:32424362, PubMed:34678144). Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner (By similarity). Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3 (By similarity). Involved in innate immune response by acting as an integral adapter in the assembly of various inflammasomes (NLRP2, NLRP3, NLRP6 and AIM2) which recruit and activate caspase-1 leading to processing and secretion of pro-inflammatory cytokines (PubMed:15190255, PubMed:15507117, PubMed:21892172, PubMed:22555457, PubMed:32424362, PubMed:34678144). Caspase-1-dependent inflammation leads to macrophage pyroptosis, a form of cell death (By similarity). The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions (By similarity). Clustered PYCARD nucleates the formation of caspase-1 filaments through the interaction of their respective CARD domains, acting as a platform for of caspase-1 polymerization (By similarity). In the NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1 (By similarity). In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation (By similarity). May be involved in RIGI-triggered pro-inflammatory responses and inflammasome activation (By similarity). In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8 (PubMed:22555457). In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2; the latter function is proposed to involve the nuclear form (By similarity). Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome; this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways (By similarity). For regulation of NF-kappa-B activating and inhibiting functions have been reported (By similarity). Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK (By similarity). Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing (By similarity). Modulates host resistance to DNA virus infection, probably by inducing the cleavage of and inactivating CGAS in presence of cytoplasmic double-stranded DNA (PubMed:28314590).

Alternative names

Asc, Pycard, Apoptosis-associated speck-like protein containing a CARD, mASC, PYD and CARD domain-containing protein

Recommended products

Rabbit Recombinant Monoclonal TMS1/ASC antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR28201-61
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Immunoprecipitation - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (ab307561)
This data was developed using Anti-TMS1/ASC antibody [EPR28201-61] ab307560, the same antibody clone in a different buffer formulation.
TMS1/ASC was immunoprecipitated from 0.35 mg J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate 10 ug with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-TMS1/ASC antibody [EPR28201-61] ab307560 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate 10 ug
Lane 2: ABAB307560 IP in J774A.1 whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-TMS1/ASC antibody [EPR28201-61] ab307560 in J774A.1 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 50 seconds
All lanes: Immunoprecipitation - Anti-TMS1/ASC antibody [EPR28201-61] (Anti-TMS1/ASC antibody [EPR28201-61] ab307560) at 1/1000 dilution
Lane 1: J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate 10 μg
Lane 2: J774A.1 whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 21 kDa
Exposure time: 50s
Western blot - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (ab307561)
This data was developed using 307560, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Negative control: RAW 264.7 (PMID:28766178);
Low expression tissue: skeletal muscle (PMID:32659370).
Lanes 1-6: 15 seconds;
Lane 7: 125 seconds.
Exposure time:
All lanes: Western blot - Anti-TMS1/ASC antibody [EPR28201-61] (Anti-TMS1/ASC antibody [EPR28201-61] ab307560) at 1/1000 dilution
Lane 1: J774A.1 (mouse reticum cell sarcoma monocyte/macrophage ) whole cell lysate 20 μg
Lane 2: RAW 264.7 (mouse elson murine leukemia virus-induced tumor macrophage) whole cell lysate 20 μg
Lane 3: Mouse spleen tissue lysate 20 μg
Lane 4: Mouse colon tissue lysate 20 μg
Lane 5: Mouse skeletal muscle tissue lysate 20 μg
Lane 6: Rat spleen tissue lysate 20 μg
Lane 7: Rat colon tissue lysate 20 μg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 21 kDa
Exposure time: 15s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (ab307561)
This data was developed using Anti-TMS1/ASC antibody [EPR28201-61] ab307560, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling TMS1/ASC with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 at 1/1000 (0.484 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on rat spleen.The section was incubated with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (ab307561)
This data was developed using Anti-TMS1/ASC antibody [EPR28201-61] ab307560, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling TMS1/ASC with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 at 1/1000 (0.484 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Low expression tissue: no staining on rat skeletal muscle.The section was incubated with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (ab307561)
This data was developed using Anti-TMS1/ASC antibody [EPR28201-61] ab307560, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling TMS1/ASC with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 at 1/1000 (0.484 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on rat colon.The section was incubated with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (ab307561)
This data was developed using Anti-TMS1/ASC antibody [EPR28201-61] ab307560, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling TMS1/ASC with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 at 1/1000 (0.484 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on mouse spleen.The section was incubated with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (ab307561)
This data was developed using Anti-TMS1/ASC antibody [EPR28201-61] ab307560, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling TMS1/ASC with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 at 1/1000 (0.484 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Low expression tissue: no staining on mouse skeletal muscle.The section was incubated with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (ab307561)
This data was developed using Anti-TMS1/ASC antibody [EPR28201-61] ab307560, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse head and neck tissue labeling TMS1/ASC with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 at 1/1000 (0.484 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on mouse head and neck squamous cell carcinoma.The section was incubated with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (ab307561)
This data was developed using Anti-TMS1/ASC antibody [EPR28201-61] ab307560, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling TMS1/ASC with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 at 1/1000 (0.484 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on mouse colon.The section was incubated with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (ab307561)
This data was developed using Anti-TMS1/ASC antibody [EPR28201-61] ab307560, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) J774A.1 (mouse r tissue labeling TMS1/ASC with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 at 1/1000 (0.484 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on (A) J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) cell pellet, no staining on (B) RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cell pellet.The section was incubated with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (ab307561)
This data was developed using Anti-TMS1/ASC antibody [EPR28201-61] ab307560, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) cells lABelling TMS1/ASC with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 at 1/500 (0.968 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear and cytoplasmic staining in J774A.1 cell line. Negative control: RAW 264.7 (PMID: 28766178Ј©Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/ml dilution.
Flow Cytometry (Intracellular) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (ab307561)
This data was developed using Anti-TMS1/ASC antibody [EPR28201-61] ab307560, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeABilized RAW264.7 (mouse ABelson murine leukemia virus-induced tumor macrophage, Left) / J774A.1 (mouse reticulum cell sarcoma monocyte macrophage, Right) cells lABelling TMS1/ASC with Anti-TMS1/ASC antibody [EPR28201-61] ab307560 at 1/500 dilution (0.1ug) (Red) (Red) compared with a RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlABelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-RABbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: RAW 264.7 (PMID: 28766178).