AB309497

Anti-TMS1/ASC antibody [RM1049]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
KO Validated
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(33 Publications)

Anti-TMS1/ASC antibody [RM1049] (ab309497) is a rabbit recombinant multiclonal antibody detecting TMS1/ASC in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

ASC, CARD5, TMS1, PYCARD, Apoptosis-associated speck-like protein containing a CARD, hASC, Caspase recruitment domain-containing protein 5, PYD and CARD domain-containing protein, Target of methylation-induced silencing 1

20 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon cancer.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue : No staining on human skeletal muscle.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [RM1049] (AB309497)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling TMS1/ASC with ab309497 at 1/500 (0.996 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing nuclear and cytoplasmic staining in THP-1 cell line. Negative control : Hela (PMID : 10567338).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

Flow Cytometry (Intracellular) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hela (human cervical adenocarcinoma epithelial cell, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labelling TMS1/ASC with ab309497 at 1/5000 dilution (0.01 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : Hela (PMID : 10567338).

Supplier Data

Immunoprecipitation - Anti-TMS1/ASC antibody [RM1049] (AB309497)

TMS1/ASC was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate with ab309497 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309497 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : THP-1 (human monocytic leukemia monocyte) whole cell lysate Lane 2 : ab309497 IP in THP-1 (human monocytic leukemia monocyte) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab309497 in THP-1 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 10 seconds TMS1/ASC has four isoforms. 24-kDa ASC and 17-kDa ASC-b are observed after immunoprecipitation. The molecular weight observed is consistent with what has been described in the literature (PMID : 20482797).

All lanes:

Immunoprecipitation - Anti-TMS1/ASC antibody [RM1049] (ab309497) at 1/30 dilution

All lanes:

THP-1 (human monocytic leukemia monocyte) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 10s

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat spleen.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) cells labelling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing nuclear and cytoplasmic staining in J774A.1 cell line. Negative control : RAW 264.7 (PMID : 28766178). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue : No staining on mouse skeletal muscle (PMID : 32659370).The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue : No staining on rat skeletal muscle.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemical analysis of paraffin-embedded Mouse head and neck tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse head and neck squamous cell cancer.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat colon.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse colon.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse spleen.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Left) / J774A.1(mouse reticulum cell sarcoma monocyte/macrophage, Right) cells labelling TMS1/ASC with ab309497 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : RAW 264.7 (PMID : 28766178)

Supplier Data

Immunoprecipitation - Anti-TMS1/ASC antibody [RM1049] (AB309497)

TMS1/ASC was immunoprecipitated from 0.35 mg J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate with ab309497 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309497 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate Lane 2 : ab309497 IP in J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab309497 in J774A.1 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-TMS1/ASC antibody [RM1049] (ab309497) at 1/30 dilution

All lanes:

J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 10s

Supplier Data

Western blot - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : HeLa (PMID : 10567338). TMS1/ASC has four isoforms. 24-kDa ASC, 17-kDa ASC-b and 12-kDa ASC-c are observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 20482797). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 125 seconds

All lanes:

Western blot - Anti-TMS1/ASC antibody [RM1049] (ab309497) at 1/1000 dilution

Lane 1:

Parental HAP1 whole cell lysate at 20 µg

Lane 2:

TMS1/ASC knockout HAP1 whole cell lysate at 20 µg

Lane 3:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 4:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 12 kDa,17 kDa,24 kDa

false

Exposure time: 125s

Supplier Data

Western blot - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression tissue : skeletal muscle (PMID : 32659370). TMS1/ASC has four isoforms. 24-kDa ASC, 17-kDa ASC-b and 12-kDa ASC-c are observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 20482797). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 125 seconds

All lanes:

Western blot - Anti-TMS1/ASC antibody [RM1049] (ab309497) at 1/1000 dilution

Lane 1:

Human spleen tissue lysate at 20 µg

Lane 2:

Human colon tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 12 kDa,17 kDa,24 kDa

false

Exposure time: 125s

Supplier Data

Western blot - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : RAW 264.7 (PMID : 28766178). Low expression tissue : skeletal muscle (PMID : 32659370) TMS1/ASC has four isoforms. 24-kDa ASC, 17-kDa ASC-b and 12-kDa ASC-c are observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 20482797). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 26 seconds

All lanes:

Western blot - Anti-TMS1/ASC antibody [RM1049] (ab309497) at 1/1000 dilution

Lane 1:

J774A.1 (mouse reticum cell sarcoma monocyte/macrophage ) whole cell lysate 20 at 20 µg

Lane 2:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Mouse skeletal muscle at 20 µg

Lane 5:

Rat spleen tissue lysate at 20 µg

Lane 6:

Rat colon tissue lysate at 20 µg

Lane 7:

Rat skeletal muscle at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 12 kDa,17 kDa,24 kDa

false

Exposure time: 26s

Carrier free

Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM1049

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

WB, ICC/IF, IHC-P, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-TMS1/ASC antibody [RM1049] (ab309497) is a rabbit recombinant recombinant multiclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-TMS1/ASC antibody [RM1049] (ab309497) has been confirmed by Western blot testing in PYCARD Knockout HAP1 cell line.

Other related products
We have a range of other formats of antibody clone [RM1049] also available for your convenience: ab309497, Carrier free - ab309498

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a key mediator in apoptosis and inflammation (PubMed : 11103777, PubMed : 12646168, PubMed : 15030775, PubMed : 17349957, PubMed : 17599095, PubMed : 19158675, PubMed : 19158676, PubMed : 19234215, PubMed : 19494289, PubMed : 21487011, PubMed : 24630722, PubMed : 25847972, PubMed : 30674671, PubMed : 34678144, PubMed : 36050480). Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner (PubMed : 11103777, PubMed : 12646168). Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3 (PubMed : 14730312, PubMed : 16964285). Involved in innate immune response by acting as an integral adapter in the assembly of various inflammasomes (NLRP1, NLRP2, NLRP3, NLRP6, AIM2 and probably IFI16) which recruit and activate caspase-1 leading to processing and secretion of pro-inflammatory cytokines (PubMed : 15030775, PubMed : 16982856, PubMed : 17349957, PubMed : 17599095, PubMed : 19158675, PubMed : 19158676, PubMed : 19234215, PubMed : 21487011, PubMed : 23530044, PubMed : 24630722, PubMed : 25847972, PubMed : 29440442, PubMed : 30674671, PubMed : 33980849, PubMed : 34678144, PubMed : 34706239). Caspase-1-dependent inflammation leads to macrophage pyroptosis, a form of cell death (PubMed : 24630722). The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions (PubMed : 14499617, PubMed : 19234215, PubMed : 24630722). Clustered PYCARD nucleates the formation of caspase-1 filaments through the interaction of their respective CARD domains, acting as a platform for of caspase-1 polymerization (PubMed : 24630722). In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1 (PubMed : 17349957). In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation (PubMed : 16964285). May be involved in RIGI-triggered pro-inflammatory responses and inflammasome activation (PubMed : 19915568). In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8 (PubMed : 19158675, PubMed : 19158676). In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2; the latter function is proposed to involve the nuclear form (PubMed : 22732093). Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome; this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways (PubMed : 12486103, PubMed : 16585594). For regulation of NF-kappa-B activating and inhibiting functions have been reported (PubMed : 12486103). Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK (PubMed : 12486103, PubMed : 16585594). Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing (PubMed : 16585594). Modulates host resistance to DNA virus infection, probably by inducing the cleavage of and inactivating CGAS in presence of cytoplasmic double-stranded DNA (PubMed : 28314590).. Isoform 2. May have a regulating effect on the function as inflammasome adapter.. Isoform 3. Seems to inhibit inflammasome-mediated maturation of interleukin-1 beta.

See full target information PYCARD

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:9623-9642 PubMed41041065

2025

ASIC1a Induces Excessive Mitophagy and PANoptosis of Chondrocyte by the Inhibition of SIRT3 Mitochondrial Translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoyan Zai,Xuewen Qian,Yayun Xu,Huifang Lv,Mengjia Hao,Yueming Tao,Lixin Rui,Xiaoyue Zhang,Xiaoqing Peng,Yihao Zhang,Feihu Chen

Scientific reports 15:33776 PubMed41028781

2025

OGT-mediated O-GlcNAc of FOXO1 promotes the progression of neonatal heart failure via regulating pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Wu,Zhiliang Xu,Xiaoou Li,Bing He,Qingyan Zhao

Journal of nanobiotechnology 23:600 PubMed40890823

2025

Circadian disruption and ROS-NLRP3 signaling mediate sleep deprivation-enhanced silica nanoparticle toxicity in lacrimal glands.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxiao Zhang,Di Qi,Xiaoting Pei,Dingli Lu,Mengru Ba,Shuting Xuan,Duliurui Huang,Tingting Yang,Jingwen Yang,Zhijie Li,Shenzhen Huang

Foods (Basel, Switzerland) 14: PubMed40870677

2025

Yellow Pigments Ameliorate Hyperuricemia via Dual Mechanisms: Xanthine Oxidase Inhibition and Uric Acid Transporter Modulation (ABCG2, URAT1, and GLUT9).

Applications

Unspecified application

Species

Unspecified reactive species

Furong Xue,Renqin Zhu,Jiaxing Li,Zheng Liu,Lidan Niu,Wei Chen,Chengtao Wang,Jie Zheng

Bioactive materials 51:825-840 PubMed40678266

2025

Dual-pathway targeted therapy for Parkinson's disease: Biomimetic nanosomes inhibit ferroptosis and pyroptosis through NLRP3 inflammasome regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wang,Xuemei Liao,Qinglong Guo,Heng Zhang,Lei Ye,Liangchen Yu,Xiaoming Kong,Yicheng Jiang,Peng Zhao,Kaiyong Cai,Hongwei Cheng

BMC medicine 23:407 PubMed40624500

2025

Vagus nerve stimulation inhibits pyroptosis and improves outcome of cerebral ischemic stroke by upregulating osteopontin (SPP1) to disturb ASC oligomerization.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wen,Hao Tang,Ling Wang,Qinghuan Yang,Yong Zhao,Yu Ren,Ting Qin,Xuemei Li,Jianfeng Xu,Gongwei Jia,Qin Yang

Psychopharmacology : PubMed40493075

2025

Ginsenoside Rg1 alleviates PCPA-induced insomnia by inhibiting NLRP3 inflammasome activation and pyroptosis through the Nrf2/HO-1 pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Li,Xiufeng Wang,Yu Zhang,Min Wei,Jianqiang Qi,Dan Liu,Runhua Wu,Qin Chen,Junshan Huang

International journal of molecular sciences 26: PubMed40362361

2025

Effect of Diethylstilbestrol on Implantation and Decidualization in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Ran,Si-Ting Chen,Meng-Yuan Li,Dan-Dan Jin,Zeng-Ming Yang

Drug development research 86:e70083 PubMed40198768

2025

Sirtuin 1 Is a Potential Target for the Treatment of Neurogenic Intermittent Claudication by Modulating Pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejian Dan,Hong Wu,Wei Liu,Xiao Hu,Wei Xu,Chen Li,Bin Ma

Drug design, development and therapy 19:1909-1926 PubMed40098903

2025

Design of a Highly Active Peptide Inhibitor of Farnesyltransferase and Its Protective Effect Against Acute Liver Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Lian Huang,Hang-Shuai Qu,A-Li Li,Chen-Qian Ying,Hui Shao,Yong-Zhi Tang,Hua-Zhong Chen,Tao-Hsin Tung,Jian-Sheng Zhu

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Anti-TMS1/ASC antibody [RM1049]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Flow Cytometry (Intracellular) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunoprecipitation - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Flow Cytometry (Intracellular) - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Immunoprecipitation - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Western blot - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Western blot - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Western blot - Anti-TMS1/ASC antibody [RM1049] (AB309497)

Related conjugates and formulations (1)

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Immunogen

Complementary Products

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Primary Antibodies

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Publications (33)

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