AB309498

Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Multiclonal TMS1/ASC antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples.

View Alternative Names

ASC, CARD5, TMS1, PYCARD, Apoptosis-associated speck-like protein containing a CARD, hASC, Caspase recruitment domain-containing protein 5, PYD and CARD domain-containing protein, Target of methylation-induced silencing 1

20 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue : No staining on human skeletal muscle.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon cancer.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling TMS1/ASC with ab309497 at 1/500 (0.996 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing nuclear and cytoplasmic staining in THP-1 cell line. Negative control : Hela (PMID : 10567338).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

Flow Cytometry (Intracellular) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Flow cytometric analysis of Hela (human cervical adenocarcinoma epithelial cell, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labelling TMS1/ASC with ab309497 at 1/5000 dilution (0.01 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : Hela (PMID : 10567338).

Supplier Data

Immunoprecipitation - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. TMS1/ASC was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate with ab309497 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309497 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : THP-1 (human monocytic leukemia monocyte) whole cell lysate Lane 2 : ab309497 IP in THP-1 (human monocytic leukemia monocyte) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab309497 in THP-1 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 10 seconds TMS1/ASC has four isoforms. 24-kDa ASC and 17-kDa ASC-b are observed after immunoprecipitation. The molecular weight observed is consistent with what has been described in the literature (PMID : 20482797).

All lanes:

Immunoprecipitation - Anti-TMS1/ASC antibody [RM1049] (<a href='/en-us/products/primary-antibodies/tms1-asc-antibody-rm1049-ab309497'>ab309497</a>) at 1/30 dilution

All lanes:

THP-1 (human monocytic leukemia monocyte) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 10s

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) cells labelling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing nuclear and cytoplasmic staining in J774A.1 cell line. Negative control : RAW 264.7 (PMID : 28766178?Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue : No staining on mouse skeletal muscle (PMID : 32659370).The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue : No staining on rat skeletal muscle.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse head and neck tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse head and neck squamous cell cancer.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat colon.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse colon.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse spleen.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling TMS1/ASC with ab309497 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat spleen.The section was incubated with ab309497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Flow cytometric analysis of RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Left) / J774A.1(mouse reticulum cell sarcoma monocyte/macrophage, Right) cells labelling TMS1/ASC with ab309497 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : RAW 264.7 (PMID : 28766178)

Supplier Data

Immunoprecipitation - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. TMS1/ASC was immunoprecipitated from 0.35 mg J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate with ab309497 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309497 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate Lane 2 : ab309497 IP in J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab309497 in J774A.1 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-TMS1/ASC antibody [RM1049] (<a href='/en-us/products/primary-antibodies/tms1-asc-antibody-rm1049-ab309497'>ab309497</a>) at 1/30 dilution

All lanes:

J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 10s

Supplier Data

Western blot - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : HeLa (PMID : 10567338). TMS1/ASC has four isoforms. 24-kDa ASC, 17-kDa ASC-b and 12-kDa ASC-c are observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 20482797). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 125 seconds

All lanes:

Western blot - Anti-TMS1/ASC antibody [RM1049] (<a href='/en-us/products/primary-antibodies/tms1-asc-antibody-rm1049-ab309497'>ab309497</a>) at 1/1000 dilution

Lane 1:

Parental HAP1 whole cell lysate at 20 µg

Lane 2:

TMS1/ASC knockout HAP1 whole cell lysate at 20 µg

Lane 3:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 4:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 12 kDa,17 kDa,24 kDa

false

Exposure time: 125s

Supplier Data

Western blot - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression tissue : skeletal muscle (PMID : 32659370). TMS1/ASC has four isoforms. 24-kDa ASC, 17-kDa ASC-b and 12-kDa ASC-c are observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 20482797). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 125 seconds

All lanes:

Western blot - Anti-TMS1/ASC antibody [RM1049] (<a href='/en-us/products/primary-antibodies/tms1-asc-antibody-rm1049-ab309497'>ab309497</a>) at 1/1000 dilution

Lane 1:

Human spleen tissue lysate at 20 µg

Lane 2:

Human colon tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 12 kDa,17 kDa,24 kDa

false

Exposure time: 125s

Supplier Data

Western blot - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

This data was developed using ab309497, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : RAW 264.7 (PMID : 28766178). Low expression tissue : skeletal muscle (PMID : 32659370) TMS1/ASC has four isoforms. 24-kDa ASC, 17-kDa ASC-b and 12-kDa ASC-c are observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 20482797). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 26 seconds

All lanes:

Western blot - Anti-TMS1/ASC antibody [RM1049] (<a href='/en-us/products/primary-antibodies/tms1-asc-antibody-rm1049-ab309497'>ab309497</a>) at 1/1000 dilution

Lane 1:

J774A.1 (mouse reticum cell sarcoma monocyte/macrophage ) whole cell lysate 20 at 20 µg

Lane 2:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Mouse skeletal muscle at 20 µg

Lane 5:

Rat spleen tissue lysate at 20 µg

Lane 6:

Rat colon tissue lysate at 20 µg

Lane 7:

Rat skeletal muscle at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 12 kDa,17 kDa,24 kDa

false

Exposure time: 26s

Unconjugated

Anti-TMS1/ASC antibody [RM1049]

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM1049

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB, ICC/IF, IHC-P, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a key mediator in apoptosis and inflammation (PubMed : 11103777, PubMed : 12646168, PubMed : 15030775, PubMed : 17349957, PubMed : 17599095, PubMed : 19158675, PubMed : 19158676, PubMed : 19234215, PubMed : 19494289, PubMed : 21487011, PubMed : 24630722, PubMed : 25847972, PubMed : 30674671, PubMed : 34678144, PubMed : 36050480). Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner (PubMed : 11103777, PubMed : 12646168). Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3 (PubMed : 14730312, PubMed : 16964285). Involved in innate immune response by acting as an integral adapter in the assembly of various inflammasomes (NLRP1, NLRP2, NLRP3, NLRP6, AIM2 and probably IFI16) which recruit and activate caspase-1 leading to processing and secretion of pro-inflammatory cytokines (PubMed : 15030775, PubMed : 16982856, PubMed : 17349957, PubMed : 17599095, PubMed : 19158675, PubMed : 19158676, PubMed : 19234215, PubMed : 21487011, PubMed : 23530044, PubMed : 24630722, PubMed : 25847972, PubMed : 29440442, PubMed : 30674671, PubMed : 33980849, PubMed : 34678144, PubMed : 34706239). Caspase-1-dependent inflammation leads to macrophage pyroptosis, a form of cell death (PubMed : 24630722). The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions (PubMed : 14499617, PubMed : 19234215, PubMed : 24630722). Clustered PYCARD nucleates the formation of caspase-1 filaments through the interaction of their respective CARD domains, acting as a platform for of caspase-1 polymerization (PubMed : 24630722). In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1 (PubMed : 17349957). In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation (PubMed : 16964285). May be involved in RIGI-triggered pro-inflammatory responses and inflammasome activation (PubMed : 19915568). In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8 (PubMed : 19158675, PubMed : 19158676). In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2; the latter function is proposed to involve the nuclear form (PubMed : 22732093). Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome; this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways (PubMed : 12486103, PubMed : 16585594). For regulation of NF-kappa-B activating and inhibiting functions have been reported (PubMed : 12486103). Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK (PubMed : 12486103, PubMed : 16585594). Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing (PubMed : 16585594). Modulates host resistance to DNA virus infection, probably by inducing the cleavage of and inactivating CGAS in presence of cytoplasmic double-stranded DNA (PubMed : 28314590).. Isoform 2. May have a regulating effect on the function as inflammasome adapter.. Isoform 3. Seems to inhibit inflammasome-mediated maturation of interleukin-1 beta.

See full target information PYCARD

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Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Flow Cytometry (Intracellular) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Immunoprecipitation - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Flow Cytometry (Intracellular) - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Immunoprecipitation - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Western blot - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Western blot - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

Western blot - Anti-TMS1/ASC antibody [RM1049] - BSA and Azide free (AB309498)

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