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AB250408

Anti-TMUB2 antibody [EPR13741] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TMUB2 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

FP2653, UNQ1897/PRO4343, TMUB2, Transmembrane and ubiquitin-like domain-containing protein 2

3 Images
Flow Cytometry (Intracellular) - Anti-TMUB2 antibody [EPR13741] - BSA and Azide free (AB250408)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TMUB2 antibody [EPR13741] - BSA and Azide free (AB250408)

This data was developed using ab181183, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of TMUB2 expression in HeLa cells using ab181183 at 1/150 dilution (red) and a rabbit IgG as negative control (green).

Immunoprecipitation - Anti-TMUB2 antibody [EPR13741] - BSA and Azide free (AB250408)
  • IP

Supplier Data

Immunoprecipitation - Anti-TMUB2 antibody [EPR13741] - BSA and Azide free (AB250408)

This data was developed using ab181183, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) or negative control (lane 2), labeling TMUB2 immunoprecipitated using ab181183 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-TMUB2 antibody [EPR13741] (<a href='/en-us/products/primary-antibodies/tmub2-antibody-epr13741-ab181183'>ab181183</a>)

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Western blot - Anti-TMUB2 antibody [EPR13741] - BSA and Azide free (AB250408)
  • WB

Supplier Data

Western blot - Anti-TMUB2 antibody [EPR13741] - BSA and Azide free (AB250408)

This data was developed using ab181183, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TMUB2 antibody [EPR13741] (<a href='/en-us/products/primary-antibodies/tmub2-antibody-epr13741-ab181183'>ab181183</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HEK293 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: Oct-34 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13741

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Based on the sequence analysis, this antibody recognizes 4 isoforms with predicted MW of 34KDa, 32KDa, 19KDa and 10KDa, respectively.

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Complementary Products

Proteins & Peptides

AB276574

Recombinant Human TMUB2 protein (His tag)

SDS-PAGE - Recombinant Human TMUB2 protein (His tag) (AB276574)

  • 0
  • 0
Primary Antibodies

AB85550

Anti-PMP70 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody (AB85550)

  • 0
  • 0
Primary Antibodies

AB180586

Anti-TMUB1 antibody [EPR14066]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-TMUB1 antibody [EPR14066] (AB180586)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab250408 is the carrier-free version of ab181183.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information TMUB2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com