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AB9348

Anti-TNF alpha antibody [2.1_4E10-1H11]

2

(1 Review)

|

(7 Publications)

Mouse Monoclonal TNF alpha antibody. Suitable for Flow Cyt, sELISA, IHC-P and reacts with Recombinant fragment - Human, Human, Recombinant full length protein - Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNF.

View Alternative Names

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

1 Images
Sandwich ELISA - Anti-TNF alpha antibody [2.1_4E10-1H11] (AB9348)
  • sELISA

Unknown

Sandwich ELISA - Anti-TNF alpha antibody [2.1_4E10-1H11] (AB9348)

In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000 pg/ml of Recombinant Human TNF-α when used with suitable detection antibody at a concentration of at least 0.5-1.0 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2.1_4E10-1H11

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

sELISA, Flow Cyt, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TNF.

P01375

Reactivity data

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Product details

This product is no longer batch tested in IHC, for an IHC validated antibody please see ab1793.

Monoclonal antibodies were produced in BALB/c x C57BL/6 F1 mice

Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 500µL
Purification technique
Affinity purification Protein G
Purification notes
This antibody was purified from cell culture by Protein G affinity chromatography.
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

The TNF protein, primarily secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces cell death in specific tumor cell lines and acts as a potent pyrogen, causing fever directly or by stimulating interleukin-1 secretion. TNF is implicated in cachexia induction and can stimulate cell proliferation and differentiation under certain conditions. It impairs regulatory T-cells (Treg) function in rheumatoid arthritis patients through FOXP3 dephosphorylation, upregulating protein phosphatase 1 (PP1), which dephosphorylates 'Ser-418' of FOXP3, inactivating FOXP3 and leading to defective Treg cells. TNF is a key mediator of cell death in the anticancer effect of BCG-stimulated neutrophils with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. It induces insulin resistance in adipocytes by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake. TNF plays a role in angiogenesis by inducing VEGF production with IL1B and IL6. Additionally, the TNF intracellular domain (ICD) form stimulates IL12 production in dendritic cells. This supplementary information is collated from multiple sources and compiled automatically.
See full target information TNF

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 12: PubMed36978830

2023

Voltage-Gated Proton Channel Hv1 Regulates Neuroinflammation and Dopaminergic Neurodegeneration in Parkinson's Disease Models.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew L Neal,Eric E Beier,Muhammad M Hossain,Alexa Boyle,Jiaying Zheng,Chunki Kim,Isha Mhatre-Winters,Long-Jun Wu,Jason R Richardson

Frontiers in pharmacology 13:1037983 PubMed36467083

2022

An orally available small molecule that targets soluble TNF to deliver anti-TNF biologic-like efficacy in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Vugler,James O'Connell,Mai Anh Nguyen,Dietmar Weitz,Thomas Leeuw,Elizabeth Hickford,Alexander Verbitsky,Xiaoyou Ying,Markus Rehberg,Bruce Carrington,Mark Merriman,Andrew Moss,Jean-Marie Nicholas,Phil Stanley,Sara Wright,Tim Bourne,Yann Foricher,Daniel Brookings,Helen Horsley,Matthias Herrmann,Srinivas Rao,Markus Kohlmann,Peter Florian

Immunology 163:185-200 PubMed33480040

2021

Montelukast alleviates inflammation in experimental autoimmune encephalomyelitis by altering Th17 differentiation in a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Han,Yan-Yan Zhang,Ze-Qing Ye,Yun Xiao,Javad Rasouli,Wen-Cheng Wu,Su-Min Ye,Xin-Yue Guo,Lin Zhu,Abdolmohamad Rostami,Li-Bin Wang,Yuan Zhang,Xing Li

Biosensors 10: PubMed33228090

2020

Developing Rapid Antimicrobial Susceptibility Testing for Motile/Non-Motile Bacteria Treated with Antibiotics Covering Five Bactericidal Mechanisms on the Basis of Bead-Based Optical Diffusometry.

Applications

Unspecified application

Species

Unspecified reactive species

Yao-Tzu Yang,Jhih-Cheng Wang,Han-Sheng Chuang

Evidence-based complementary and alternative medicine : eCAM 2019:4674190 PubMed31379962

2019

Ameliorates Rheumatic Heart Disease by Affecting Relative Percentages of CD4CD25FoxP3 Treg and CD4IL17 T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tiechao Jiang,Qini Zhao,Hongyan Sun,Lirong Zhang,Shanshan Song,Hongli Chi,Hui Zhou

Neurobiology of disease 108:115-127 PubMed28823928

2017

Alternative microglial activation is associated with cessation of progressive dopamine neuron loss in mice systemically administered lipopolysaccharide.

Applications

Unspecified application

Species

Unspecified reactive species

Eric E Beier,Matthew Neal,Gelerah Alam,Melissa Edler,Long-Jun Wu,Jason R Richardson

Scientific reports 7:46336 PubMed28402317

2017

Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform.

Applications

IA

Species

Unspecified reactive species

Chih-Yao Chung,Jhih-Cheng Wang,Han-Sheng Chuang
View all publications

Product promise

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