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AB1793

Anti-TNF alpha antibody [52B83]

4

(19 Reviews)

|

(378 Publications)

Anti-TNF alpha antibody [52B83] (ab1793) is a mouse monoclonal antibody detecting TNF alpha in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-Fr, ICC/IF, ELISA. Suitable for Guinea pig, Human, Mouse, Rhesus monkey.

- Over 270 publications
- Trusted since 2002

View Alternative Names

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (AB1793)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (AB1793)

ab1793 staining TNFα in RAW 264.7 cells treated with (R,S)-rolipram (ab120029), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R,S)-rolipram, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120029 ((R,S)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Flow Cytometry (Intracellular) - Anti-TNF alpha antibody [52B83] (AB1793)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TNF alpha antibody [52B83] (AB1793)

Overlay histogram showing RAW 264.7 cells stained with ab1793 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1793, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in RAW 264.7 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (AB1793)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (AB1793)

ab1793 staining TNFα in RAW 264.7 cells treated with (R)-(-)-rolipram (ab120031), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R)-(-)-rolipram , as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120031 ((R)-(-)--rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (AB1793)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (AB1793)

ab1793 staining TNFα in RAW 264.7 cells treated with (S)-(+)-rolipram (ab120030), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (S)-(+)-rolipram , as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120030 ((S)-(+)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793(5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

52B83

Isotype

IgG1

Light chain type

unknown

Carrier free

No

Reacts with

Mouse, Rhesus monkey, Guinea pig, Human

Applications

ELISA, Flow Cyt (Intra), ICC/IF, IHC-Fr, WB

applications

Immunogen

Native Full Length Protein corresponding to Human TNF.

P01375

Specificity

This antibody detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. It reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. It does not react with receptor-bound TNF-alpha. Non-specific background staining is observed in connective tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p>TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p>(methanol fixed cells)</p><p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "2 µg/mL", "IHCFr-species-notes": "<p>Fixed in acetone for 10 minutes</p>" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p>TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10", "FlowCytIntra-species-notes": "<p>(methanol fixed cells)</p><p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "2 µg/mL", "IHCFr-species-notes": "<p>Fixed in acetone for 10 minutes</p>" }, "Guinea pig": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p>TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p>(methanol fixed cells)</p><p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "2 µg/mL", "IHCFr-species-notes": "<p>Fixed in acetone for 10 minutes</p>" }, "Rhesus monkey": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p>TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p>(methanol fixed cells)</p><p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "2 µg/mL", "IHCFr-species-notes": "<p>Fixed in acetone for 10 minutes</p>" } } }
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Product details

Product Specifications
Anti-TNF alpha antibody [52B83] (ab1793) is a mouse monoclonal antibody and is validated for use in ELISA, Flow Cyt (Intra), ICC/IF, IHC-Fr, WB in guineapig, human, mouse, rhesus monkey samples.
Anti-TNF alpha antibody [52B83] (ab1793) specifically detects TNF alpha (UniProt ID: P01375; Molecular weight: 26kDa) and is sold in 50 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-TNF alpha antibody [52B83] (ab1793) has high sensitivity and specificity.
Anti-TNF alpha antibody [52B83] (ab1793) has been cited over 275 times in peer reviewed journals and is trusted by the scientific community.
Anti-TNF alpha antibody [52B83] (ab1793) has 16 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone 52B83 - ab233027.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The TNF protein, primarily secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces cell death in specific tumor cell lines and acts as a potent pyrogen, causing fever directly or by stimulating interleukin-1 secretion. TNF is implicated in cachexia induction and can stimulate cell proliferation and differentiation under certain conditions. It impairs regulatory T-cells (Treg) function in rheumatoid arthritis patients through FOXP3 dephosphorylation, upregulating protein phosphatase 1 (PP1), which dephosphorylates 'Ser-418' of FOXP3, inactivating FOXP3 and leading to defective Treg cells. TNF is a key mediator of cell death in the anticancer effect of BCG-stimulated neutrophils with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. It induces insulin resistance in adipocytes by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake. TNF plays a role in angiogenesis by inducing VEGF production with IL1B and IL6. Additionally, the TNF intracellular domain (ICD) form stimulates IL12 production in dendritic cells. This supplementary information is collated from multiple sources and compiled automatically.
See full target information TNF
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Publications (378)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastroenterology 31:104277 PubMed40900774

2025

Sex based relative expression of estrogen receptors and tumor necrosis factor-alpha in liver affects hepatitis C virus viral pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Groover,Sarah Addison,Savannah Nicks,Mitchelle Mwangi,Amy Brooks,Anil Kaul,Rashmi Kaul

Journal of nanobiotechnology 23:559 PubMed40790215

2025

Dynamic three-dimensional culture enhances tunneling nanotubes-mediated mitochondrial transfer in mesenchymal stromal cells to accelerate wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Ma,Xiaoxue Yang,Xiaoyao Huang,Hao Guo,Zihan Li,Siyuan Fan,Han Qin,Fanhui Meng,Peisheng Liu,Xinyu Wang,Meiling Wu,Kun Xuan,Anqi Liu

Frontiers in endocrinology 16:1620032 PubMed40747306

2025

Bioinformatic analysis identifies LPL as a critical gene in diabetic kidney disease via lipoprotein metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Dong,Huan Xu,Pengjie Xu,Jiang Liu,Zhouji Shen

Science advances 11:eadv4238 PubMed40712009

2025

Genetically engineered chondrocyte-mimetic nanoplatform attenuates osteoarthritis by blocking IL-1β and restoring sirtuin-3.

Applications

Unspecified application

Species

Unspecified reactive species

Caifeng Deng,Liukang Yu,Xuan Zhao,Yuxiao Chen,Jiabin Mei,Jie Wei,Xiaoyuan Chen,Guanghua Lei,Chao Zeng

Frontiers in medicine 12:1569590 PubMed40630478

2025

Network pharmacology-based approach to research the effects and mechanisms of injection against idiopathic pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Liangyu Chen,Haobo Lin,Linmang Qin,Guangfeng Zhang,Peisheng Chen,Zebo Jiang,Pan Xu,Donghui Huang,Xiao Zhang

BMC biology 23:176 PubMed40598379

2025

Astaxanthin prevents postovulatory oocyte aging by targeting TNFR2 and inhibiting the TNF signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wen,Zhe Han,Qi Yang,Ke-Song Shi,Dui Sun,Xiao-Jie Zhang,Cheng-Guang Liang

Marine drugs 23: PubMed40559659

2025

Rhamnan Sulfate from the Seaweed May Improve Cognitive Impairment Caused by Hyperglycemia.

Applications

Unspecified application

Species

Unspecified reactive species

Takaichi Miwa,Masaya Sato,Ning Ma,Keiichi Hiramoto,Masahiro Terasawa,Koji Suzuki

Frontiers in cellular and infection microbiology 15:1538461 PubMed40496014

2025

SARS-CoV-2 exploits steroidogenic machinery, triggers lipid metabolism for viral replication and induces immune response in Leydig cells of K18-hACE2 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Salmo Azambuja de Oliveira,André Acácio Souza da Silva,Barry T Hinton,Giovanni Freitas Gomes,Thiago Mattar Cunha,Paulo Sérgio Cerri,Estela Sasso-Cerri

Signal transduction and targeted therapy 10:182 PubMed40484878

2025

Single-cell transcriptional dissection illuminates an evolution of immunosuppressive microenvironment during pancreatic ductal adenocarcinoma metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowei Liu,Jinen Song,Meiling Yuan,Fengli Zuo,Huihui Li,Leyi Tang,Xinmin Wang,Xueyan Wang,Qian Xiao,Li Li,Xinyu Liu,Zhankun Yang,Jianlin Wu,Jing Jing,Xuelei Ma,Hubing Shi

Frontiers in immunology 16:1570692 PubMed40469287

2025

Neuroinflammation associated with proviral DNA persists in the brain of virally suppressed people with HIV.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah J Byrnes,Janna Jamal Eddine,Jingling Zhou,Emily Chalmers,Emma Wanicek,Narin Osman,Trisha A Jenkins,Michael Roche,Bruce J Brew,Jacob D Estes,Thomas A Angelovich,Melissa J Churchill
View all publications
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