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Mouse Monoclonal TNF alpha antibody. Suitable for ELISA, WB, ICC/IF, Flow Cyt (Intra), IHC-Fr and reacts with Mouse, Guinea pig, Rhesus monkey, Human samples. Cited in 274 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human TNF.
Alternative names=Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF, TNFA, TNFSF2
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Native Full Length Protein corresponding to Human TNF. Database link: P01375
Reactivity data
Select an application| ELISA | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Expected | Expected | Not recommended | Expected | Expected | Expected |
| Mouse | Expected | Expected | Not recommended | Tested | Tested | Expected |
| Guinea pig | Expected | Expected | Not recommended | Expected | Expected | Expected |
| Rhesus monkey | Expected | Expected | Not recommended | Expected | Expected | Expected |
Images
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Guinea pig, Rhesus monkey, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2 µg/mL | Notes TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used. |
Species Guinea pig | Dilution info 2 µg/mL | Notes TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used. |
Species Rhesus monkey | Dilution info 2 µg/mL | Notes TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used. |
Species Human | Dilution info 2 µg/mL | Notes TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha is 17.4 kDa A reduced sample treatment and 15% SDS-Page was used. |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Guinea pig, Rhesus monkey, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Guinea pig, Rhesus monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10 | Notes (methanol fixed cells) ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes (methanol fixed cells) ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species Guinea pig | Dilution info - | Notes (methanol fixed cells) ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species Rhesus monkey | Dilution info - | Notes (methanol fixed cells) ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2 µg/mL | Notes Fixed in acetone for 10 minutes |
Species Guinea pig | Dilution info 2 µg/mL | Notes Fixed in acetone for 10 minutes |
Species Rhesus monkey | Dilution info 2 µg/mL | Notes Fixed in acetone for 10 minutes |
Species Human | Dilution info 2 µg/mL | Notes Fixed in acetone for 10 minutes |
Target data
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918, PubMed:16829952, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed:12794819).The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Recommended products
Mouse Monoclonal TNF alpha antibody. Suitable for ELISA, WB, ICC/IF, Flow Cyt (Intra), IHC-Fr and reacts with Mouse, Guinea pig, Rhesus monkey, Human samples. Cited in 274 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human TNF.
Alternative names=Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF, TNFA, TNFSF2
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Native Full Length Protein corresponding to Human TNF. Database link: P01375
- Clone number
52B83
- Purification technique
Affinity purification Protein G
- Specificity
This antibody detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. It reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. It does not react with receptor-bound TNF-alpha. Non-specific background staining is observed in connective tissues.
- Light chain type
unknown
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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