AB286149

Anti-TNF alpha antibody

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(1 Publication)

Rabbit Polyclonal TNF alpha antibody. Suitable for WB, Neutralization and reacts with Recombinant full length protein - Rat, Rat samples. Cited in 1 publication.

View Alternative Names

Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

1 Images

Supplier Data

Western blot - Anti-TNF alpha antibody (AB286149)

All lanes:

Western blot - Anti-TNF alpha antibody (ab286149) at 4 µg/mL

Lane 1:

Recombinant rat TNF alpha, 10 ng

Lane 2:

Recombinant rat TNF alpha, 50 ng

Lane 3:

Recombinant rat TNF alpha, 250 ng

Predicted band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

Neutralization, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Neutralization" : {"fullname" : "Neutralization", "shortname":"Neutralization"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "Neutralization-species-checked": "guaranteed", "Neutralization-species-dilution-info": "2-10 µg/mL", "Neutralization-species-notes": "<p></p>" }, "Recombinant full length protein - Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "Neutralization-species-checked": "notRecommended", "Neutralization-species-dilution-info": "", "Neutralization-species-notes": "" } } }

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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 3054 TNF-alpha Antibody. 3054-100 is the same size as the 100 µg size of ab286149.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.01% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

See full target information Tnf

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Kaohsiung journal of medical sciences 39:801-810 PubMed37265208

2023

Plantamajoside modulates immune dysregulation and hepatic lipid metabolism in rats with nonalcoholic fatty liver disease via AMPK/Nrf2 elevation.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Mu Wu,Getu Zhaori,Li Mei,Xiao-Man Ren,Arong Tong Laga,Batu Deligen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TNF alpha antibody

Western blot - Anti-TNF alpha antibody (AB286149)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Complementary Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise