AB66579

Anti-TNF alpha antibody

Name: Anti-TNF alpha antibody (ab66579) | Abcam
Brand: Abcam Limited
SKU: AB66579
Price: 400 USD
Availability: InStock
Rating: 4 (10 reviews)

Lab Essentials
What is this?

(10 Reviews)

(141 Publications)

Anti-TNF alpha antibody (ab66579) is a rabbit polyclonal antibody detecting TNF alpha in Western Blot, ICC/IF. Suitable for Human, Mouse.

- Over 140 publications
- Trusted since 2009

View Alternative Names

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

2 Images

Lab

Western blot - Anti-TNF alpha antibody (AB66579)

Mature THP-1 and Raw264.7 cells were treated with LPS (1 ug/ml) for 6 hours to stimulate production of TNF alpha. Brefeldin A (300 ng/ml) was added in the final 3 hours of incubation to inhibit golgi traffic.

All lanes:

Western blot - Anti-TNF alpha antibody (ab66579) at 1 µg/mL

Lane 1:

THP1 cells treated with PMA (negative control) at 20 µg

Lane 2:

THP1 cells treated with PMA+LPS+Brefeldin A at 20 µg

Lane 3:

Raw264.7 cells treated with PMA (negative control) at 20 µg

Lane 4:

Raw264.7 cells treated with PMA+LPS+Brefeldin A at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 25 kDa

Observed band size: 26 kDa,28 kDa,50 kDa,75 kDa

false

Exposure time: 2min

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody (AB66579)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody (AB66579)

ab66579 staining TNF alpha in Raw264.7 cells. The cells were treated with LPS for 7 hours and Brefeldin A (ab120299) for the final 3 hours. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab66579 at 1μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 4% paraformaldehyde (10 min).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Ab66579 has been batch tested in Western Blot using LPS stimulated lysates. Abcam does not recommend this product for use in IHC. Please contact Abcam Scientific Support for more information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "Abcam recommends using 3% Milk as the blocking agent.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "Abcam recommends using 3% Milk as the blocking agent.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Baboon": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The TNF protein, primarily secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces cell death in specific tumor cell lines and acts as a potent pyrogen, causing fever directly or by stimulating interleukin-1 secretion. TNF is implicated in cachexia induction and can stimulate cell proliferation and differentiation under certain conditions. It impairs regulatory T-cells (Treg) function in rheumatoid arthritis patients through FOXP3 dephosphorylation, upregulating protein phosphatase 1 (PP1), which dephosphorylates 'Ser-418' of FOXP3, inactivating FOXP3 and leading to defective Treg cells. TNF is a key mediator of cell death in the anticancer effect of BCG-stimulated neutrophils with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. It induces insulin resistance in adipocytes by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake. TNF plays a role in angiogenesis by inducing VEGF production with IL1B and IL6. Additionally, the TNF intracellular domain (ICD) form stimulates IL12 production in dendritic cells. This supplementary information is collated from multiple sources and compiled automatically.

See full target information TNF

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Publications (141)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmacologica Sinica 45:339-353 PubMed37816857

2023

A dual role of lysophosphatidic acid type 2 receptor (LPAR2) in nonsteroidal anti-inflammatory drug-induced mouse enteropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Hutka,Anett Várallyay,Szilvia B László,András S Tóth,Bálint Scheich,Sándor Paku,Imre Vörös,Zoltán Pós,Zoltán V Varga,Derek D Norman,Andrea Balogh,Zoltán Benyó,Gábor Tigyi,Klára Gyires,Zoltán S Zádori

Phytotherapy research : PTR 37:5854-5870 PubMed37655750

2023

Galangin attenuates doxorubicin-induced cardiotoxicity via activating nuclear factor erythroid 2-related factor 2/heme oxygenase 1 signaling pathway to suppress oxidative stress and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyao Fang,Xiuchuan Li,Fengyuan Yang,Ting Huang,Chenming Qiu,Ke Peng,Yongjian Yang,Cong Lan

iScience 26:107754 PubMed37731617

2023

Aggravated hepatic fibrosis induced by phenylalanine and tyrosine was ameliorated by chitooligosaccharides supplementation.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Liu,Heng Li,Hongyu Xu,Jinsong Gong,Min Jiang,Zhenghong Xu,Jinsong Shi

Current issues in molecular biology 45:1287-1305 PubMed36826029

2023

Effects of Extract (Brainon) on Aging-Induced Memory Impairment in SAMP8 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hae Lim Kim,Sung Kwon Lee,Da Eun Min,Tonking Bastola,Bo Yoon Chang,Jin Hye Bae,Dong Ryung Lee

Psychopharmacology 240:347-359 PubMed36633660

2023

Sex-differences in anxiety, neuroinflammatory markers, and enhanced fear learning following chronic heroin withdrawal.

Applications

Unspecified application

Species

Unspecified reactive species

Shveta V Parekh,Lydia O Adams,Gillian A Barkell,Donald T Lysle

Archives of oral biology 147:105612 PubMed36603515

2023

Receptor-interacting protein 1 inhibition prevents mechanical stress-induced temporomandibular joint osteoarthritis by regulating apoptosis and later-stage necroptosis of chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yiwen Zhou,Shuang Lin,Ziwei Huang,Caixia Zhang,Huijuan Wang,Baochao Li,Huang Li

Molecular biology reports 50:2243-2255 PubMed36572761

2022

Pre exposure to enriched environment alleviates brain injury after ischemia-reperfusion by inhibiting p38MAPK/STAT1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Ya Shen,Yu Han,Zhen-Kun Gao,Ping-Ping Han,Xia Bi

Brain, behavior, & immunity - health 26:100542 PubMed36388136

2022

MDMA administration attenuates hippocampal IL-β immunoreactivity and subsequent stress-enhanced fear learning: An animal model of PTSD.

Applications

Unspecified application

Species

Unspecified reactive species

Shveta V Parekh,Lydia O Adams,Gillian A Barkell,Donald T Lysle

Evidence-based complementary and alternative medicine : eCAM 2022:3255401 PubMed36262166

2022

Fufang Fanshiliu Decoction Revealed the Antidiabetic Effect through Modulating Inflammatory Response and Gut Microbiota Composition.

Applications

Unspecified application

Species

Unspecified reactive species

Leyu Li,Guoxin Huang,Tingbo Chen,Hui Lin,Ruiyan Xu,Jinyan Cheng,Ying Hu,Weibo Dai,Gengting Dong

EBioMedicine 84:104275 PubMed36152520

2022

Endothelial caveolin-1 regulates cerebral thrombo-inflammation in acute ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohao Zhang,Pengyu Gong,Ying Zhao,Ting Wan,Kang Yuan,Yunyun Xiong,Min Wu,Mingming Zha,Yunzi Li,Teng Jiang,Xinfeng Liu,Ruidong Ye,Yi Xie,Gelin Xu

View all publications

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We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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