AB6671

Anti-TNF alpha antibody

Name: anti-TNF alpha antibody - rabbit polyclonal (ab6671) | Abcam
Brand: Abcam Limited
SKU: AB6671
Price: 400 USD
Availability: InStock
Rating: 4 (35 reviews)

Lab Essentials
What is this?

(35 Reviews)

(1021 Publications)

Anti-TNF alpha antibody (ab6671) is a rabbit polyclonal antibody detecting TNF alpha in Western Blot, IHC-P, ICC/IF, ELISA, Neut. Suitable for Cynomolgus monkey, Human,.

- Over 840 publications
- Trusted since 2002

View Alternative Names

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

1 Images

Supplier Data

Western blot - Anti-TNF alpha antibody (AB6671)

Western Blot analysis, labeling TNF alpha with ab6671 at 1/1000. Blocking was with 1% BSA in TBS-T incubated for 30 minutes at room temperature. The cleavage fragment is related to the soluble isoform 77 - 223.

All lanes:

Western blot - Anti-TNF alpha antibody (ab6671) at 1/1000 dilution

All lanes:

Recombinant TNF alpha protein

Secondary

All lanes:

Goat Anti-Rabbit Dylight® 649 Conjugate at 1/20000 dilution

Predicted band size: 25 kDa

Observed band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Cynomolgus monkey

Applications

ELISA, IF, ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TNF.

P01375

Specificity

The immunogen used to raise this antibody shares 78% homology with mouse and rat TNF alpha protein. Some customers have successfully used ab6671 with mouse and rat samples, however we do not batch test in these species and therefore we cannot guarantee this product will consistently work with mouse and rat. We would recommend ab205587 or ab1793 as an alternative product for use with these species.

The antibody does not recognize human TNF beta (lymphotoxin).

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Reactivity data

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1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IF-species-checked": "predicted", "IF-species-dilution-info": "", "IF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IF-species-checked": "predicted", "IF-species-dilution-info": "", "IF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/1000 - 1/5000", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IF-species-checked": "guaranteed", "IF-species-dilution-info": "", "IF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IF-species-checked": "predicted", "IF-species-dilution-info": "", "IF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IF-species-checked": "predicted", "IF-species-dilution-info": "", "IF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IF-species-checked": "predicted", "IF-species-dilution-info": "", "IF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Primates": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IF-species-checked": "predicted", "IF-species-dilution-info": "", "IF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IF-species-checked": "guaranteed", "IF-species-dilution-info": "", "IF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "Membrane Blocking is recommended with BSA not Milk for this product.Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli.", "IF-species-checked": "notRecommended", "IF-species-dilution-info": "", "IF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Endotoxin content by LAL is <10 pg/ml.

Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077). Or search our wide range of secondary antibodies for use with your experiment.

Product Specifications
Anti-TNF alpha antibody (ab6671) is a rabbit polyclonal antibody and is validated for use in ELISA, ICC/IF, IF, IHC-P, Neut, WB in cynomolgus monkey, human, samples.
Anti-TNF alpha antibody (ab6671) specifically detects TNF alpha (UniProt ID: P01375; Molecular weight: 26kDa) and is sold in 200 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-TNF alpha antibody (ab6671) has high sensitivity and specificity.
Anti-TNF alpha antibody (ab6671) has been cited over 846 times in peer reviewed journals and is trusted by the scientific community.
Anti-TNF alpha antibody (ab6671) has 35 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone - ab174646.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

This antibody is primarily directed against mature 17,000 MW human TNFα and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFα in the same formats using similar dilutions.

Storage buffer

pH: 7.2 Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The TNF protein, primarily secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces cell death in specific tumor cell lines and acts as a potent pyrogen, causing fever directly or by stimulating interleukin-1 secretion. TNF is implicated in cachexia induction and can stimulate cell proliferation and differentiation under certain conditions. It impairs regulatory T-cells (Treg) function in rheumatoid arthritis patients through FOXP3 dephosphorylation, upregulating protein phosphatase 1 (PP1), which dephosphorylates 'Ser-418' of FOXP3, inactivating FOXP3 and leading to defective Treg cells. TNF is a key mediator of cell death in the anticancer effect of BCG-stimulated neutrophils with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. It induces insulin resistance in adipocytes by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake. TNF plays a role in angiogenesis by inducing VEGF production with IL1B and IL6. Additionally, the TNF intracellular domain (ICD) form stimulates IL12 production in dendritic cells. This supplementary information is collated from multiple sources and compiled automatically.

See full target information TNF

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Publications (1021)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:648 PubMed41074162

2025

Fe-MOF loaded with capmatinib to reverse immunosuppressive microenvironment and inhibit tumor metastasis after radiofrequency ablation.

Applications

Unspecified application

Species

Unspecified reactive species

Jinchao Zhao,Lei Lei,Cheng Zeng,Xianfei Zhou,Junkang Ding,Wenbin Dai,Angfeng Jiang,Shaomin Zou,Qiao Jin,Zhe Tang

International journal of tryptophan research : IJTR 18:11786469251372797 PubMed41035962

2025

Neuroactive Kynurenine Metabolite Alterations Unveil Novel Association to Locomotor Deterioration in Diabetic Neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandra Perez-Alvarez,Victor Salazar,Gustavo Bruges,Eva Vonasek,Antonio Eblen-Zajjur

Materials today. Bio 35:102272 PubMed40994624

2025

An injectable phosphocreatine-grafted hydrogel incorporating hierarchically structured teriparatide/SrZnP-functionalized Zn-Cu particles for osteogenesis-angiogenesis coupling and osteoporotic bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Haotian Qin,Zhiping Guan,Yuanhao Wang,Jin Zhao,Zhenhai Xie,Huaiyu Li,Chen Zhang,Weibei Sheng,Fei Yu,Jian Weng,Yingqi Chen,Deli Wang,Hui Zeng,Junyu Qian

Foods (Basel, Switzerland) 14: PubMed40870713

2025

Low-Dose, Post-Storage Dancong Tea Attenuates Hydroalcohol-Induced Gastric Damage via Modulation Antioxidant and Anti-Inflammatory Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Huanwei Jian,Ruohong Chen,Lingli Sun,Qiuhua Li,Junxi Cao,Xingfei Lai,Zhenbiao Zhang,Suwan Zhang,Mengjiao Hao,Shili Sun,Zhongzheng Chen

Journal of translational medicine 23:924 PubMed40826460

2025

AAV-mediated STC-1 expression mitigates neuroinflammation and preserves visual function in degenerative retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yange Wang,Siyu Li,Chenxu Zhang,Miao Li,Kexin Wang,Gang Wang,Ye Tao,Zongming Song

Frontiers in immunology 16:1618012 PubMed40821844

2025

CRIF1 gene therapy ameliorates inflammatory bowel disease by suppressing TH17 cells and fibrosis through mitochondrial function regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Sil Park,Hye Yeon Kang,Ha Yeon Jeong,SeungCheon Yang,JeongWon Choi,Sang Hee Cho,Sol Kim,Bo-In Lee,Mi-La Cho

Journal of inflammation research 18:10711-10722 PubMed40809463

2025

Multi-Omics Analysis Identifies Immune Regulatory Networks in Sepsis-Associated Liver Injury: Experimental Validation and Clinical Relevance.

Applications

Unspecified application

Species

Unspecified reactive species

Yiyu Hong,Qiutong Chen,Hua Xie,Mingliu Ma,Siting Gan,Yuqi Zhang,Zhaozhong Xu

Bioactive materials 53:459-479 PubMed40747455

2025

Metformin carbon dots-based osteogenic and protein delivery system to promote bone regeneration in periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Wei,Kai Wang,Yongkai Li,Jiao Huang,Ping Deng,Xianbo Xia,Cong Yang,Ling Xu,Junji Xu

BMC veterinary research 21:486 PubMed40702451

2025

Protective effects of folic acid on ischemia‒reperfusion injury in rat uterus: reduces inflammation damage and apoptotic death.

Applications

Unspecified application

Species

Unspecified reactive species

Zahid Paksoy,Nihat Yumusak

Science advances 11:eadp0467 PubMed40601753

2025

Single-cell analysis of the decidua unveils the mechanism of anti-inflammatory exosomes for chorioamnionitis in nonhuman primates.

Applications

Unspecified application

Species

Unspecified reactive species

Seung Hyun Jang,Hojun Choi,Eun Mi Lee,Seung-Bin Yoon,Hye-Jung Kim,Cheolhyoung Park,Jae Kyung Won,Seung Han Shin,Ji-Su Kim,Chan-Wook Park,Heon Yung Gee,Chulhee Choi

View all publications

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