Rabbit Polyclonal TNF alpha antibody. Suitable for ELISA, WB, IF, IHC-P, Neut, ICC/IF and reacts with Recombinant fragment, Human, Cynomolgus monkey, Recombinant full length protein - Human samples. Cited in 846 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNF.
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Publications
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- Journal of inflammation (London, England) 20:462023SARS-CoV-2 spike protein accelerates systemic sclerosis by increasing inflammatory cytokines, Th17 cells, and fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHa Yeon Jeong,Jin-Sil Park,Jin Seok Woo,Kun Hee Lee,Jeong Won Choi,Hye Yeon Kang,Hyun Sik Na,Yeon Su Lee,In Gyu Um,Sung-Hwan Park,Mi-La ChoPubMed 38129904
- NPJ biofilms and microbiomes 9:992023Spinal cord injury-induced gut dysbiosis influences neurological recovery partly through short-chain fatty acids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingli Jing,Degang Yang,Fan Bai,Qiuying Wang,Chao Zhang,Yitong Yan,Zihan Li,Yan Li,Zhiguo Chen,Jianjun Li,Yan YuPubMed 38092763
- Frontiers in neuroscience 17:12651342023Anxiolytic and antidepressant like effects of Zamzam water in STZ-induced diabetic rats, targeting oxidative stress, neuroinflammation, BDNF/ERK/CREP pathway with modulation of hypothalamo-pituitary-adrenal axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMedhat Taha,Mohamed Ezzat Mahmoud,Abdullah G Al-Kushi,Anas Sarhan,Omer Abdelbagi,Tourki A S Baokbah,Omar Babateen,Ibrahim El-Shenbaby,Naeem F Qusty,Sara T ElazabPubMed 38105928
- Arthritis research & therapy 25:2302023Resatorvid alleviates experimental inflammatory TMJOA by restraining chondrocyte pyroptosis and synovial inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Liu,Huimin Li,Yaping Feng,Huilin Guo,Yingjie Li,Jin Ke,Xing LongPubMed 38031141
- Aging 15:11740-1176320231,5-anhydro-D-fructose induces anti-aging effects on aging-associated brain diseases by increasing 5'-adenosine monophosphate-activated protein kinase activity via the peroxisome proliferator-activated receptor-γ co-activator-1α/brain-derived neurotrophic factor pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKiyoshi Kikuchi,Shotaro Otsuka,Seiya Takada,Kazuki Nakanishi,Kentaro Setoyama,Harutoshi Sakakima,Eiichiro Tanaka,Ikuro MaruyamaPubMed 37950725
- International journal of molecular sciences 24:2023Enhanced Intervertebral Disc Repair via Genetically Engineered Mesenchymal Stem Cells with Tetracycline Regulatory System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYeji Kim,Seong Bae An,Sang-Hyuk Lee,Jong Joo Lee,Sung Bum Kim,Jae-Cheul Ahn,Dong-Youn Hwang,Inbo HanPubMed 38003216
- Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 18:628-6392023GPNMB Ameliorates Neuroinflammation Via the Modulation of AMPK/NFκB Signaling Pathway After SAH in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Li,Yuansheng Zhang,Qixiong Lu,Li Lei,Jingshu Du,Xiaoyang LuPubMed 37919457
- Cells 12:2023The Proinflammatory Role of ANGPTL8 R59W Variant in Modulating Inflammation through NF-κB Signaling Pathway under TNFα Stimulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohamed Abu-Farha,Dhanya Madhu,Prashantha Hebbar,Anwar Mohammad,Arshad Channanath,Sina Kavalakatt,Nada Alam-Eldin,Fatima Alterki,Ibrahim Taher,Osama Alsmadi,Mohammad Shehab,Hossein Arefanian,Rasheed Ahmad,Thangavel Alphonse Thanaraj,Fahd Al-Mulla,Jehad AbubakerPubMed 37947641
- Scientific reports 13:187042023Facile fabrication of carboxymethylcellulose/ZnO/g-C3N4 containing nutmeg extract with photocatalytic performance for infected wound healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaysa Youshi,Mohammad Reza Farahpour,Zohreh Ghazi TabatabaeiPubMed 37907545
- PLoS pathogens 19:e10117432023Periodontitis promotes bacterial extracellular vesicle-induced neuroinflammation in the brain and trigeminal ganglion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJae Yeong Ha,Jiwon Seok,Suk-Jeong Kim,Hye-Jin Jung,Ka-Young Ryu,Michiko Nakamura,Il-Sung Jang,Su-Hyung Hong,Youngkyun Lee,Heon-Jin LeePubMed 37871107
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate
- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human TNF. Database link P01375
Consider this alternative
Reactivity data
Select an application| ELISA | WB | IF | IHC-P | Neut | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Expected | Predicted | Expected | Expected | Expected | Expected |
| Mouse | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Cynomolgus monkey | Expected | Predicted | Expected | Expected | Expected | Expected |
| Dog | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Monkey | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Primates | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Recombinant fragment | Expected | Not recommended | Expected | Expected | Expected | Expected |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
Species Cynomolgus monkey | Dilution info 1/1000 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Pig, Monkey, Primates | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes Membrane Blocking is recommended with BSA not Milk for this product. Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Pig, Monkey, Primates, Human, Cynomolgus monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Human, Cynomolgus monkey | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Pig, Monkey, Primates | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info 1/100 - 1/500 | Notes - |
Species Human | Dilution info 1/100 - 1/500 | Notes - |
Species Cynomolgus monkey | Dilution info 1/100 - 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Pig, Monkey, Primates | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Neut
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Human, Cynomolgus monkey | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Pig, Monkey, Primates | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Human, Cynomolgus monkey | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Pig, Monkey, Primates | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918, PubMed:16829952, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed:12794819).The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Alternative names
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF, TNFA, TNFSF2
Recommended products
Rabbit Polyclonal TNF alpha antibody. Suitable for ELISA, WB, IF, IHC-P, Neut, ICC/IF and reacts with Recombinant fragment, Human, Cynomolgus monkey, Recombinant full length protein - Human samples. Cited in 846 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNF.
Alternative names
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF, TNFA, TNFSF2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate
- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human TNF. Database link P01375
- Purity
IgG fraction
- Specificity
The immunogen used to raise this antibody shares 78% homology with mouse and rat TNF alpha protein. Some customers have successfully used ab6671 with mouse and rat samples, however we do not batch test in these species and therefore we cannot guarantee this product will consistently work with mouse and rat. We would recommend ab205587 or ab1793 as an alternative product for use with these species.
The antibody does not recognize human TNF beta (lymphotoxin).
- Concentration
- Purification notes
This antibody is primarily directed against mature 17,000 MW human TNF? and is useful in determining its presence in various assays. In general, this antibody also detects primate TNF? in the same formats using similar dilutions.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Endotoxin content by LAL is <10 pg/ml.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Or search our wide range of secondary antibodies for use with your experiment.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-TNF alpha antibody (ab6671)
Western Blot analysis, labeling TNF alpha with ab6671 at 1/1000. Blocking was with 1% BSA in TBS-T incubated for 30 minutes at room temperature. The cleavage fragment is related to the soluble isoform 77 - 223.
All lanes: Western blot - Anti-TNF alpha antibody (ab6671) at 1/1000 dilution
All lanes: Recombinant TNF alpha protein
SecondaryAll lanes: Goat Anti-Rabbit Dylight® 649 Conjugate at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 16 kDa
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