Rabbit Polyclonal TNF alpha antibody. Suitable for WB, sELISA and reacts with Recombinant fragment - Mouse samples. Cited in 110 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Tnf aa 50 to C-terminus.
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- Translational neurodegeneration 12:502023Intranasal administration of induced pluripotent stem cell-derived cortical neural stem cell-secretome as a treatment option for Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyunkyung Mo,Juryun Kim,Jennifer Yejean Kim,Jang Woon Kim,Heeju Han,Si Hwa Choi,Yeri Alice Rim,Ji Hyeon JuPubMed 37946307
- Journal of neuroinflammation 20:2032023RGS5 augments astrocyte activation and facilitates neuroinflammation via TNF signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu Yin,Xin-Yue Ma,Ying-Feng Sun,Yan-Qing Yin,Ying Long,Chun-Lai Zhao,Jun-Wei Ma,Sen Li,Yan Hu,Ming-Tao Li,Gang Hu,Jia-Wei ZhouPubMed 37674228
- Cancers 15:2023Synthesis of New Chromene Derivatives Targeting Triple-Negative Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAysha Alneyadi,Zohra Nausheen Nizami,Hanan E Aburawi,Soleiman Hisaindee,Muhammad Nawaz,Samir Attoub,Gaber Ramadan,Nehla Benhalilou,Mazoun Al Azzani,Yassine Elmahi,Aysha Almeqbali,Khalid Muhammad,Ali H Eid,Ranjit Vijayan,Rabah IratniPubMed 37345018
- International journal of molecular sciences 24:2023Investigating Therapeutic Effects of Indole Derivatives Targeting Inflammation and Oxidative Stress in Neurotoxin-Induced Cell and Mouse Models of Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYa-Jen Chiu,Chih-Hsin Lin,Chung-Yin Lin,Pei-Ning Yang,Yen-Shi Lo,Yu-Chieh Chen,Chiung-Mei Chen,Yih-Ru Wu,Ching-Fa Yao,Kuo-Hsuan Chang,Guey-Jen Lee-ChenPubMed 36768965
- Antioxidants (Basel, Switzerland) 11:2022Ethanolic Extract Alleviates Inflammation and Modifies Gut Microbiota in Mice with DSS-Induced Colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUi-Jin Bae,Ha-Na Jang,Sung-Hyen Lee,Ji-Young Kim,Gi-Chang KimPubMed 36552637
- Journal of the American Heart Association 11:e0225742022Inflammatory Role of Milk Fat Globule-Epidermal Growth Factor VIII in Age-Associated Arterial Remodeling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeng Ni,Lijuan Liu,Wanqu Zhu,Richard Telljohann,Jing Zhang,Robert E Monticone,Kimberly R McGraw,Changwei Liu,Christopher H Morrell,Pablo Garrido-Gil,Jose Luis Labandeira-Garcia,Edward G Lakatta,Mingyi WangPubMed 36000422
- Frontiers in cellular neuroscience 16:8802672022Electroacupuncture improves TBI dysfunction by targeting HDAC overexpression and BDNF-associated Akt/GSK-3β signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShih-Ya Hung,Hsin-Yi Chung,Sih-Ting Luo,Yu-Ting Chu,Yu-Hsin Chen,Iona J MacDonald,Szu-Yu Chien,Peddanna Kotha,Liang-Yo Yang,Ling-Ling Hwang,Nae J Dun,De-Maw Chuang,Yi-Hung ChenPubMed 36016833
- Brain sciences 12:2022Lipocalin-2 Deficiency Reduces Hepatic and Hippocampal Triggering Receptor Expressed on Myeloid Cells-2 Expressions in High-Fat Diet/Streptozotocin-Induced Diabetic Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun Joo Shin,Zhen Jin,Hyeong Seok An,Gyeongah Park,Jong Youl Lee,So Jeong Lee,Hye Min Jang,Eun Ae Jeong,Kyung Eun Kim,Jaewoong Lee,Dae Young Yoo,Gu Seob RohPubMed 35884685
- PloS one 17:e02632542022Oral administration of bovine lactoferrin suppresses the progression of rheumatoid arthritis in an SKG mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShunryou Yanagisawa,Karin Nagasaki,Chanbora Chea,Toshinori Ando,Nurina Febriyanti Ayuningtyas,Toshihiro Inubushi,Atsushi Ishikado,Hiromichi Imanaka,Eiji Sugiyama,Ichiro Takahashi,Mutsumi Miyauchi,Takashi TakataPubMed 35148358
- Pharmacological research 176:1060832022Xuanfei Baidu Decoction reduces acute lung injury by regulating infiltration of neutrophils and macrophages via PD-1/IL17A pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuying Wang,Xi Wang,Yixuan Li,Zhifeng Xue,Rui Shao,Lin Li,Yan Zhu,Han Zhang,Jian YangPubMed 35033647
Key facts
- Isotype
- Unknown
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Mouse Tnf aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P06804
Reactivity data
Select an application| WB | sELISA | |
|---|---|---|
| Recombinant fragment - Mouse | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes To detect TNF alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant TNF alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. The presursor is ~26 kDa and the secreted form is ~17 kDa. |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info - | Notes To detect Murine TNF alpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9739 is required. This antigen affinity purified antibody, in conjunction with ab245857 detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF alpha. |
Associated Products
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Target data
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed:25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (PubMed:32741026). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Alternative names
Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a
Recommended products
Rabbit Polyclonal TNF alpha antibody. Suitable for WB, sELISA and reacts with Recombinant fragment - Mouse samples. Cited in 110 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Tnf aa 50 to C-terminus.
Key facts
- Isotype
- Unknown
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Tnf aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P06804
- Purification technique
- Affinity purification Immunogen
- Light chain type
- unknown
- Reconstitution
- reconstitute with water at 200µL
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see Anti-TNF alpha antibody [52B83] ab1793.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-TNF alpha antibody (ab9739)
To detect mouse TNF alpha by Western Blot analysis, ab9739 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant mouse TNF alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse TNF alpha, respectively.
Non-reducing conditions.
All lanes: Western blot - Anti-TNF alpha antibody (ab9739)
Predicted band size: 25 kDa
Sandwich ELISA - Anti-TNF alpha antibody (ab9739)
ELISA analysis of ab9739. This antigen affinity purified antibody, in conjunction with a suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF alpha.
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