AB9755

Anti-TNF alpha antibody

Lab Essentials
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(27 Publications )

Rabbit Polyclonal TNF alpha antibody. Suitable for sELISA and reacts with Recombinant full length protein - Rat samples. Cited in 27 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Tnf aa 50 to C-terminus.

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Sandwich ELISA - Anti-TNF alpha antibody (AB9755)

sELISA

Supplier Data

Sandwich ELISA - Anti-TNF alpha antibody (AB9755)

Sandwich ELISA of ab9755. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rTNF-alpha.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

Unknown

Light chain type

unknown

Carrier free

Applications

sELISA

applications

Immunogen

Recombinant Fragment Protein within Rat Tnf aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P16599

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Rat": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.5-2 µg/mL", "sELISA-species-notes": "<p>To detect rTNF-alpha by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rTNF-alpha.</p>" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification technique

Affinity purification Immunogen

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

See full target information Tnf

Alternative Names

Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

International journal of general medicine 17:2655-2671 PubMed38859909

2024

Analysis of the Expression and Prognostic Value of SIRTs in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Qin,Xiaofei Ye,Hongliang Luo,Hu Jin,Qiang Liu,Jiangfa Li

Cell biochemistry and function 41:1252-1262 PubMed37787620

2023

Peripheral insulin resistance is early, progressive, and correlated with cachexia in Walker-256 tumor-bearing rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hely de Morais,Suéllen Kathiane Fernandes Vilas Bôas,Camila O de Souza,Daniele Romani Miksza,Carolina C L Moreira,Mirian Ayumi Kurauti,Flaviane de F Silva,Priscila Cassolla,Francemilson Goulart da Silva,Leticia Cristina Limiere,Sabrina Grassiolli,Roberto B Bazotte,Helenir M de Souza

The journal of obstetrics and gynaecology research 49:2417-2426 PubMed37464974

2023

Human Wharton's jelly-derived mesenchymal stem cells prevent pregnancy loss in a rat by JAK/STAT-mediated immunomodulation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoying Ding,Rongrong Wu,Beibei Jin,Chunyu Zhu,Yuquan Zhang,Xiaoqing Yang

Frontiers in bioengineering and biotechnology 9:740863 PubMed34692658

2021

Zwitterionic Hydrogel Activates Autophagy to Promote Extracellular Matrix Remodeling for Improved Pressure Ulcer Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Li,Shishuang Jiang,Liwan Song,Zhe Yao,Junwen Zhang,Kangning Wang,Liping Jiang,Huacheng He,Cai Lin,Jiang Wu

Journal of neuroinflammation 17:250 PubMed32859236

2020

Rh-relaxin-2 attenuates degranulation of mast cells by inhibiting NF-κB through PI3K-AKT/TNFAIP3 pathway in an experimental germinal matrix hemorrhage rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Li,Gang Zhao,Fanfan Chen,Yan Ding,Tianyi Wang,Shengpeng Liu,Weitian Lu,Weilin Xu,Jerry Flores,Umut Ocak,Tongyu Zhang,John H Zhang,Jiping Tang

Molecular medicine reports 22:651-660 PubMed32626927

2020

TLR4 mediates inflammation and hepatic fibrosis induced by chronic intermittent hypoxia in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Peng Lin,Hui-Li Lin,Xue-Ping Yu,Yi-Juan Zheng,Si-Yu Cheng

Journal of cellular biochemistry 121:4558-4568 PubMed32056265

2020

Akt activation by insulin treatment attenuates cachexia in Walker-256 tumor-bearing rats.

Applications

Unspecified application

Species

Unspecified reactive species

Flaviane de Fatima Silva,Hely de Morais,Milene Ortiz Silva,Francemilson Goulart da Silva,Rafael Vianna Croffi,Caroline Serrano-Nascimento,Maria Fernanda Rodrigues Graciano,Angelo Rafael Carpinelli,Roberto Barbosa Bazotte,Helenir Medri de Souza

Experimental and therapeutic medicine 19:123-130 PubMed31853281

2019

Resveratrol alleviates obesity-associated podocyte injury in ovariectomized obese rats.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Li,Xiaoyan Xiao,Yulian Miao,Ling Guo,Junhui Zhen,Xianhua Li,Bei Jiang,Zhao Hu

Frontiers in neuroscience 13:1127 PubMed31708728

2019

ApoA-I Mimetic Peptide Reduces Vascular and White Matter Damage After Stroke in Type-2 Diabetic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Wang,Rongwen Li,Alex Zacharek,Julie Landschoot-Ward,Michael Chopp,Jieli Chen,Xu Cui

Frontiers in neuroscience 13:665 PubMed31333398

2019

Single-Cell Glia and Neuron Gene Expression in the Central Amygdala in Opioid Withdrawal Suggests Inflammation With Correlated Gut Dysbiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sean J O'Sullivan,Evangelia Malahias,James Park,Ankita Srivastava,Beverly A S Reyes,Jon Gorky,Rajanikanth Vadigepalli,Elisabeth J Van Bockstaele,James S Schwaber

View all publications

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Product promise

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