Rabbit Polyclonal TNF alpha antibody. Suitable for sELISA and reacts with Recombinant full length protein - Rat samples. Cited in 25 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Tnf aa 50 to C-terminus.
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- Cell biochemistry and function 41:1252-12622023Peripheral insulin resistance is early, progressive, and correlated with cachexia in Walker-256 tumor-bearing rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHely de Morais,Suéllen Kathiane Fernandes Vilas Bôas,Camila O de Souza,Daniele Romani Miksza,Carolina C L Moreira,Mirian Ayumi Kurauti,Flaviane de F Silva,Priscila Cassolla,Francemilson Goulart da Silva,Leticia Cristina Limiere,Sabrina Grassiolli,Roberto B Bazotte,Helenir M de SouzaPubMed 37787620
- Frontiers in bioengineering and biotechnology 9:7408632021Zwitterionic Hydrogel Activates Autophagy to Promote Extracellular Matrix Remodeling for Improved Pressure Ulcer Healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Li,Shishuang Jiang,Liwan Song,Zhe Yao,Junwen Zhang,Kangning Wang,Liping Jiang,Huacheng He,Cai Lin,Jiang WuPubMed 34692658
- Journal of neuroinflammation 17:2502020Rh-relaxin-2 attenuates degranulation of mast cells by inhibiting NF-κB through PI3K-AKT/TNFAIP3 pathway in an experimental germinal matrix hemorrhage rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Li et. al.PubMed 32859236
- Molecular medicine reports 22:651-6602020TLR4 mediates inflammation and hepatic fibrosis induced by chronic intermittent hypoxia in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi-Peng Lin et. al.PubMed 32626927
- Journal of cellular biochemistry 121:4558-45682020Akt activation by insulin treatment attenuates cachexia in Walker-256 tumor-bearing rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFlaviane de Fatima Silva,Hely de Morais,Milene Ortiz Silva,Francemilson Goulart da Silva,Rafael Vianna Croffi,Caroline Serrano-Nascimento,Maria Fernanda Rodrigues Graciano,Angelo Rafael Carpinelli,Roberto Barbosa Bazotte,Helenir Medri de SouzaPubMed 32056265
- Experimental and therapeutic medicine 19:123-1302019Resveratrol alleviates obesity-associated podocyte injury in ovariectomized obese rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing Li,Xiaoyan Xiao,Yulian Miao,Ling Guo,Junhui Zhen,Xianhua Li,Bei Jiang,Zhao HuPubMed 31853281
- Frontiers in neuroscience 13:11272019ApoA-I Mimetic Peptide Reduces Vascular and White Matter Damage After Stroke in Type-2 Diabetic Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaohui Wang et. al.PubMed 31708728
- Frontiers in neuroscience 13:6652019Single-Cell Glia and Neuron Gene Expression in the Central Amygdala in Opioid Withdrawal Suggests Inflammation With Correlated Gut Dysbiosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSean J O'Sullivan et. al.PubMed 31333398
- Experimental and therapeutic medicine 18:779-7852019MicroRNA-146a improves sepsis-induced cardiomyopathy by regulating the TLR-4/NF-κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Xie et. al.PubMed 31281454
- Journal of biomedical materials research. Part B, Applied biomaterials 108:527-5372019Epidermal growth factor receptor genes are overexpressed within the periprosthetic soft-tissue around percutaneous devices: A pilot study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSujee Jeyapalina,John S Colombo,James P Beck,Jayant P Agarwal,Linda A Schmidt,Kent N BachusPubMed 31074946
Key facts
- Isotype
Unknown
- Host species
Rabbit
- Storage buffer
Standard buffer
- Form
Lyophilized
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Rat Tnf aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P16599
Reactivity data
Select an application| sELISA | |
|---|---|
| Recombinant full length protein - Rat | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Rat | Dilution info 0.50000-2.00000 µg/mL | Notes To detect rTNF-alpha by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rTNF-alpha. |
Associated Products
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Target data
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Alternative names
Tnfa, Tnfsf2, Tnf, Tnfa, Tnfsf2, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a
Recommended products
Rabbit Polyclonal TNF alpha antibody. Suitable for sELISA and reacts with Recombinant full length protein - Rat samples. Cited in 25 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Tnf aa 50 to C-terminus.
Key facts
- Isotype
Unknown
- Host species
Rabbit
- Storage buffer
Standard buffer
- Form
Lyophilized
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Rat Tnf aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P16599
- Purification technique
Affinity purification Immunogen
- Light chain type
unknown
- Reconstitution
Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Sandwich ELISA - Anti-TNF alpha antibody (ab9755)
Sandwich ELISA of ab9755. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rTNF-alpha.
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