Rabbit Recombinant Monoclonal TNF alpha antibody. Carrier free. Suitable for sELISA and reacts with Monkey, Human samples.
Images
![Sandwich ELISA - Anti-TNF alpha antibody [EPR18565-33] - BSA and Azide free (Detector) (AB242978), expandable thumbnail](https://content.abcam.com/products/images/tnf-alpha-antibody-epr18565-33-bsa-and-azide-free-detector-ab242978--sandwich-elisa-img174328.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-TNF alpha antibody [EPR18565-33] - BSA and Azide free (Detector) (AB242978)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
| Monkey | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:16829952, PubMed:22517918, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed:12794819). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Alternative names
TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a
Recommended products
Rabbit Recombinant Monoclonal TNF alpha antibody. Carrier free. Suitable for sELISA and reacts with Monkey, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18565-33
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab242978 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Monkey TNF alpha. The recommended pair for sandwich ELISA is:
Capture: Anti-TNF alpha antibody [EPR18565-29] - BSA and Azide free (Capture) ab242476, Human/Monkey TNF alpha Capture Antibody (unconjugated)
Detector: ab242978, Human/Monkey TNF alpha Detector Antibody (unconjugated)
The reference range value is 1.95 - 1000 pg/ml for human and 15.63 - 1000 pg/ml for monkey.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-TNF alpha antibody [EPR18565-33] - BSA and Azide free (Detector) (ab242978), expandable thumbnail](https://content.abcam.com/products/images/tnf-alpha-antibody-epr18565-33-bsa-and-azide-free-detector-ab242978--sandwich-elisa-img174328.jpg "Sandwich ELISA - Anti-TNF alpha antibody [EPR18565-33] - BSA and Azide free (Detector) (ab242978)")
Sandwich ELISA - Anti-TNF alpha antibody [EPR18565-33] - BSA and Azide free (Detector) (ab242978)
Sandwich ELISA - Recombinant human TNF alpha protein standard curve.
Background subtracted standard curve using Anti-TNF alpha antibody [EPR18565-29] - BSA and Azide free (Capture), Anti-TNF alpha antibody [EPR18565-33] - BSA and Azide free (Detector), and Recombinant human TNF alpha protein (Active) (Recombinant human TNF alpha protein (Active) ab259410) in sandwich ELISA.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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