AB34719

Anti-TNF alpha antibody [MP6-XT22] - BSA and Azide free

(1 Review)

(9 Publications)

Rat Monoclonal TNF alpha antibody. Carrier free. Suitable for Flow Cyt, ELISA, FuncS (Neut/Block), IHC-Fr and reacts with Mouse samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Tnf.

View Alternative Names

Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

MP6-XT22

Isotype

IgG1

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt, FuncS (Neut/Block), IHC-Fr, ELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Tnf.

P06804

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. Membrane permeabilisation is required for this application. <a href='/en-us/products/unavailable/rat-igg1-kappa-rtk2071-isotype-control-low-endotoxin-azide-free-ab18407'>ab18407</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

This clone (MP6-XT22) is reported to neutralise the effects of mouse TNF alpha.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

This antibody was purified from tissue culture supernatant.

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed : 25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (PubMed : 32741026).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

See full target information Tnf

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 15:268 PubMed39183338

2024

KCNJ16-depleted kidney organoids recapitulate tubulopathy and lipid recovery upon statins treatment.

Applications

Unspecified application

Species

Unspecified reactive species

E Sendino Garví,G J J van Slobbe,E A Zaal,J H F de Baaij,J G Hoenderop,R Masereeuw,M J Janssen,A M van Genderen

International journal of molecular sciences 22: PubMed34769092

2021

11,12 Epoxyeicosatrienoic Acid Rescues Deteriorated Wound Healing in Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Sommer,Heike Jakob,Caroline Reiche,Dirk Henrich,Jasmina Sterz,Johannes Frank,Ingo Marzi,Anna Lena Sander

Bioactive materials 6:1910-1920 PubMed33364530

2020

Design of a biofluid-absorbing bioactive sandwich-structured Zn-Si bioceramic composite wound dressing for hair follicle regeneration and skin burn wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaowenbin Zhang,Wenbo Li,Ying Liu,Zhigang Yang,Lingling Ma,Hui Zhuang,Endian Wang,Chengtie Wu,Zhiguang Huan,Feng Guo,Jiang Chang

Scientific reports 10:16931 PubMed33037288

2020

MicroRNAs as systemic biomarkers to assess distress in animal models for gastrointestinal diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Simone Kumstel,Heike Janssen-Peters,Ahmed Abdelrahman,Guanglin Tang,Ke Xiao,Nicole Ernst,Edgar Heinz Uwe Wendt,Rupert Palme,Nico Seume,Brigitte Vollmar,Thomas Thum,Dietmar Zechner

Molecular cancer therapeutics 18:1544-1554 PubMed31213507

2019

Antibody-Based Delivery of Cytokine Payloads to Carbonic Anhydrase IX Leads to Cancer Cures in Immunocompetent Tumor-Bearing Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Ziffels,Marco Stringhini,Philipp Probst,Tim Fugmann,Theo Sturm,Dario Neri

Biochimica et biophysica acta 1852:1610-8 PubMed25900786

2015

Diabetes-induced alterations in tissue collagen and carboxymethyllysine in rat kidneys: Association with increased collagen-degrading proteinases and amelioration by Cu(II)-selective chelation.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Brings,Shaoping Zhang,Yee S Choong,Sebastian Hogl,Martin Middleditch,Meder Kamalov,Margaret A Brimble,Deming Gong,Garth J S Cooper

Arthritis & rheumatology (Hoboken, N.J.) 67:1107-16 PubMed25580817

2015

Injury and subsequent regeneration of muscles for activation of local innate immunity to facilitate the development and relapse of autoimmune myositis in C57BL/6 mice.

Applications

IHC-Fr

Species

Mouse

Naoki Kimura,Shinya Hirata,Nobuyuki Miyasaka,Kimito Kawahata,Hitoshi Kohsaka

Neuropathology and applied neurobiology 40:911-32 PubMed24707814

2014

Increased levels of tumour necrosis factor alpha (TNFα) but not transforming growth factor-beta 1 (TGFβ1) are associated with the severity of congenital hydrocephalus in the hyh mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio-Jesús Jiménez,Luis-Manuel Rodríguez-Pérez,María-Dolores Domínguez-Pinos,María-Carmen Gómez-Roldán,María García-Bonilla,Ailec Ho-Plagaro,Ruth Roales-Buján,Sebastián Jiménez,María-Carmen Roquero-Mañueco,María-Isabel Martínez-León,María-Luisa García-Martín,Manuel Cifuentes,Bienvenido Ros,Miguel-Ángel Arráez,Javier Vitorica,Antonia Gutiérrez,José-Manuel Pérez-Fígares

Infection and immunity 67:4531-8 PubMed10456896

1999

Reactivation of latent tuberculosis: variations on the Cornell murine model.

Applications

Unspecified application

Species

Unspecified reactive species

C A Scanga,V P Mohan,H Joseph,K Yu,J Chan,J L Flynn

View all publications

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