Rat Monoclonal TNF alpha antibody. Suitable for ELISA and reacts with Recombinant fragment - Mouse samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Tnf.
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![ELISA - Anti-TNF alpha antibody [MP6-XT3] (AB11564), expandable thumbnail](https://content.abcam.com/products/images/tnf-alpha-antibody-mp6-xt3-ab11564--elisa-img29178.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-TNF alpha antibody [MP6-XT3] (AB11564)")
Publications
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- Frontiers in pharmacology 12:6372732021Identification of the Key Role of NF-κB Signaling Pathway in the Treatment of Osteoarthritis With Bushen Zhuangjin Decoction, a Verification Based on Network Pharmacology Approach.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunteng Xu,Hui Li,Xiaojuan He,Yanfeng Huang,Shengjie Wang,Lili Wang,Changlong Fu,Hongzhi Ye,Xihai Li,Tetsuya AsakawaPubMed 33912052
- Experimental and therapeutic medicine 20:3782-37902020Transcriptomic study of lipopolysaccharide-induced sepsis damage in a mouse heart model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCunrong Chen et. al.PubMed 32855727
- Evidence-based complementary and alternative medic 2020:91894572020Improving Small Intestinal Motility in Experimental Acute Necrotising Pancreatitis by Modulating the CPI-17/MLCP Pathway Using Chaiqin Chengqi Decoction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiqi Lin et. al.PubMed 32104199
- eLife 7:2018Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Ren Lin et. al.PubMed 29993362
- The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 22:17-212018Morroniside on anti-inflammation activities in rats following acute myocardial infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBangxing Yu,Guoxing Zhang,Yi An,Wen WangPubMed 29302208
- Molecular medicine reports 13:1010-82015Lentivirus-mediated TNF-α gene silencing and overexpression of osteoprotegerin inhibit titanium particle-induced inflammatory response and osteoclastogenesis in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Peng,Hongzhi Wang,Keguan Song,Hai Wang,Ping LiuPubMed 26648171
- PloS one 10:e01174982015Tempol treatment reduces anxiety-like behaviors induced by multiple anxiogenic drugs in rats.
- Journal of neuroscience research 92:944-542014Mulberroside A protects against ischemic impairment in primary culture of rat cortical neurons after oxygen-glucose deprivation followed by reperfusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCai-Ping Wang,Lu-Zhong Zhang,Gui-Cai Li,Yun-wei Shi,Jian-Long Li,Xiao-Chuan Zhang,Zhi-Wei Wang,Fei Ding,Xin-Miao LiangPubMed 24687774
- BMC complementary and alternative medicine 8:52008Effect of Lactobacillus acidophilus supernatants on body weight and leptin expression in rats.Applications:Unspecified applicationReactive species:RatRenato Sousa,Jaroslava Halper,Jian Zhang,Stephen J Lewis,Wan-I O LiPubMed 18284678
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Mouse Tnf. Database link P06804
Reactivity data
Select an application| ELISA | |
|---|---|
| Recombinant fragment - Mouse | Tested |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info 1/5 | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed:25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (PubMed:32741026). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Alternative names
Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a
Recommended products
Rat Monoclonal TNF alpha antibody. Suitable for ELISA and reacts with Recombinant fragment - Mouse samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Tnf.
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Mouse Tnf. Database link P06804
- Clone number
- MP6-XT3
- Purification technique
- Affinity purification Protein G
- Specificity
ab11564 recognises murine TNF alpha. Reactivity with murine TNF Beta has not yet been determined.
- Concentration
- Purification notes
Purified IgG prepared by affinity chromatography from tissue culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![ELISA - Anti-TNF alpha antibody [MP6-XT3] (ab11564), expandable thumbnail](https://content.abcam.com/products/images/tnf-alpha-antibody-mp6-xt3-ab11564--elisa-img29178.jpg "ELISA - Anti-TNF alpha antibody [MP6-XT3] (ab11564)")
ELISA - Anti-TNF alpha antibody [MP6-XT3] (ab11564)
Recombinant Mouse TNF alpha detected in a sandwich assay with Rat anti Mouse TNF alpha (ab11564) as the coating antibody; Rabbit anti Mouse TNF alpha:Biotin as the detetction antibody and Streptavidin:HRP as the final layer.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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