AB11564

Anti-TNF alpha antibody [MP6-XT3]

Lab Essentials
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(12 Publications)

Rat Monoclonal TNF alpha antibody. Suitable for ELISA and reacts with Recombinant fragment - Mouse samples. Cited in 12 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Tnf.

View Alternative Names

Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

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ELISA - Anti-TNF alpha antibody [MP6-XT3] (AB11564)

ELISA

Unknown

ELISA - Anti-TNF alpha antibody [MP6-XT3] (AB11564)

Recombinant Mouse TNF alpha detected in a sandwich assay with Rat anti Mouse TNF alpha (ab11564) as the coating antibody; Rabbit anti Mouse TNF alpha : Biotin as the detetction antibody and Streptavidin : HRP as the final layer.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

MP6-XT3

Isotype

IgG1

Carrier free

No

Applications

ELISA

Immunogen

Recombinant Full Length Protein corresponding to Mouse Tnf.

Specificity

ab11564 recognises murine TNF alpha. Reactivity with murine TNF Beta has not yet been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Mouse": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/5", "ELISA-species-notes": "<p></p>" } } }

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Product details

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified IgG prepared by affinity chromatography from tissue culture supernatant.

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed : 25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (PubMed : 32741026).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

See full target information Tnf

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 37:e23279 PubMed36541345

2022

The capsaicinoid nonivamide suppresses the inflammatory response and attenuates the progression of steatosis in a NAFLD-rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Naruemon Wikan,Jiraporn Tocharus,Chio Oka,Sivanan Sivasinprasasn,Waraluck Chaichompoo,Apichart Suksamrarn,Chainarong Tocharus

Journal of cellular physiology 237:1818-1832 PubMed34825376

2021

Melatonin attenuates reactive astrogliosis and glial scar formation following cerebral ischemia and reperfusion injury mediated by GSK-3β and RIP1K.

Applications

Unspecified application

Species

Unspecified reactive species

Nuttapong Yawoot,Jirakhamon Sengking,Piyawadee Wicha,Piyarat Govitrapong,Chainarong Tocharus,Jiraporn Tocharus

Synapse (New York, N.Y.) 75:e22219 PubMed34269482

2021

Propofol alleviates neuropathic pain in chronic constriction injury rat models via the microRNA-140-3p/Jagged-1 peptide/Notch signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Cheng,Wei Qin,Ai-Xing Yang,Feng-Feng Yan,Yu Chen,Jian-Xin Ma

Frontiers in pharmacology 12:637273 PubMed33912052

2021

Identification of the Key Role of NF-κB Signaling Pathway in the Treatment of Osteoarthritis With Bushen Zhuangjin Decoction, a Verification Based on Network Pharmacology Approach.

Applications

Unspecified application

Species

Unspecified reactive species

Yunteng Xu,Hui Li,Xiaojuan He,Yanfeng Huang,Shengjie Wang,Lili Wang,Changlong Fu,Hongzhi Ye,Xihai Li,Tetsuya Asakawa

Experimental and therapeutic medicine 20:3782-3790 PubMed32855727

2020

Transcriptomic study of lipopolysaccharide-induced sepsis damage in a mouse heart model.

Applications

Unspecified application

Species

Unspecified reactive species

Cunrong Chen,Junting Weng,Dexiang Fang,Jianfei Chen,Min Chen

Evidence-based complementary and alternative medic 2020:9189457 PubMed32104199

2020

Improving Small Intestinal Motility in Experimental Acute Necrotising Pancreatitis by Modulating the CPI-17/MLCP Pathway Using Chaiqin Chengqi Decoction.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqi Lin,Chenlong Zhang,Xiaoxin Zhang,Na Shi,Yongjian Wen,Chengxia Han,Dan Du,Tingting Liu,Tao Jin,Lihui Deng,Kun Jiang,Xiaonan Yang,Jia Guo,Anthony Philips,Robert Sutton,John A Windsor,Wei Huang,Ping Xue,Qing Xia

eLife 7: PubMed29993362

2018

Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Ren Lin,Benjamin Izar,Shu Wang,Clarence Yapp,Shaolin Mei,Parin M Shah,Sandro Santagata,Peter K Sorger

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 22:17-21 PubMed29302208

2018

Morroniside on anti-inflammation activities in rats following acute myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Bangxing Yu,Guoxing Zhang,Yi An,Wen Wang

Molecular medicine reports 13:1010-8 PubMed26648171

2015

Lentivirus-mediated TNF-α gene silencing and overexpression of osteoprotegerin inhibit titanium particle-induced inflammatory response and osteoclastogenesis in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Li Peng,Hongzhi Wang,Keguan Song,Hai Wang,Ping Liu

PloS one 10:e0117498 PubMed25793256

2015

Tempol treatment reduces anxiety-like behaviors induced by multiple anxiogenic drugs in rats.

Applications

WB

Species

Rat

Gaurav Patki,Ankita Salvi,Hesong Liu,Fatin Atrooz,Isam Alkadhi,Matthew Kelly,Samina Salim

View all publications

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