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Rat Monoclonal TNF alpha antibody. Suitable for ELISA and reacts with Recombinant fragment - Mouse samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Tnf.
Alternative names=Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tnfa, Tnf, Tnfsf2
- Isotype
IgG1
- Host species
Rat
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Recombinant Full Length Protein corresponding to Mouse Tnf. Database link: P06804
Reactivity data
Select an application| ELISA | |
|---|---|
| Recombinant fragment - Mouse | Tested |
Images
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse | Dilution info 1/5 | Notes - |
Target data
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed:25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity).The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
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Rat Monoclonal TNF alpha antibody. Suitable for ELISA and reacts with Recombinant fragment - Mouse samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Tnf.
Alternative names=Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tnfa, Tnf, Tnfsf2
Key facts
- Isotype
IgG1
- Host species
Rat
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Recombinant Full Length Protein corresponding to Mouse Tnf. Database link: P06804
- Clone number
MP6-XT3
- Purification technique
Affinity purification Protein G
- Specificity
ab11564 recognises murine TNF alpha. Reactivity with murine TNF Beta has not yet been determined.
- Concentration
- Purification notes
Purified IgG prepared by affinity chromatography from tissue culture supernatant.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle
Notes
This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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