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AB9809

Anti-TNF alpha antibody [NF-7]

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(2 Publications)

Mouse Monoclonal TNF alpha antibody NF-7. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications.

View Alternative Names

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

1 Images
Flow Cytometry - Anti-TNF alpha antibody [NF-7] (AB9809)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-TNF alpha antibody [NF-7] (AB9809)

Overlay histogram showing THP1 cells stained with ab9809 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9809, 2μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

NF-7

Isotype

IgG1

Light chain type

unknown

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TNF.

P01375

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

This antibody can be used in two-site immunoassay for the determination of human TNF alpha. It recognizes a different epitope than the monoclonal antibody ab9813. The two-site immunoassay can be used to determine TNF alpha concentration in the cultures of human peripheral blood mononuclear cells. Can also be used to neutralize TNF alpha cytotoxic activity in vitro. At a concentration of 2.5 μg/ml, antibody ab9809 causes 50% inhibition of TNF alpha mediated cytotoxicity on L929 cells.

This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR151594-8 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR151594-8 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

This antibody can be used in two-site immunoassay for the determination of human TNF alpha. It recognizes a different epitope than the monoclonal antibody ab9813. The two-site immunoassay can be used to determine TNF alpha concentration in the cultures of human peripheral blood mononuclear cells. Can also be used to neutralize TNF alpha cytotoxic activity in vitro. At a concentration of 2.5 μg/ml, antibody ab9809 causes 50% inhibition of TNF alpha mediated cytotoxicity on L929 cells.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The TNF protein, primarily secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces cell death in specific tumor cell lines and acts as a potent pyrogen, causing fever directly or by stimulating interleukin-1 secretion. TNF is implicated in cachexia induction and can stimulate cell proliferation and differentiation under certain conditions. It impairs regulatory T-cells (Treg) function in rheumatoid arthritis patients through FOXP3 dephosphorylation, upregulating protein phosphatase 1 (PP1), which dephosphorylates 'Ser-418' of FOXP3, inactivating FOXP3 and leading to defective Treg cells. TNF is a key mediator of cell death in the anticancer effect of BCG-stimulated neutrophils with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. It induces insulin resistance in adipocytes by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake. TNF plays a role in angiogenesis by inducing VEGF production with IL1B and IL6. Additionally, the TNF intracellular domain (ICD) form stimulates IL12 production in dendritic cells. This supplementary information is collated from multiple sources and compiled automatically.
See full target information TNF
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC research notes 11:126 PubMed29444697

2018

Modulation of pro-apoptotic effects and mitochondrial potential on B16F10 cells by DODAC/PHO-S liposomes.

Applications

Unspecified application

Species

Unspecified reactive species

Arthur Cássio de Lima Luna,José Roberto de Assis Santos Filho,Henrique Hesse,Salvador Claro Neto,Gilberto Orivaldo Chierice,Durvanei Augusto Maria

Cancer immunology, immunotherapy : CII 66:523-535 PubMed28184968

2017

Regulation of PD-L1 expression on murine tumor-associated monocytes and macrophages by locally produced TNF-α.

Applications

Unspecified application

Species

Unspecified reactive species

Genevieve Hartley,Daniel Regan,Amanda Guth,Steven Dow
View all publications
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