AB269772

Anti-TNF alpha antibody [TNFA/1172] - BSA and Azide free

Name: Anti-TNF alpha antibody [TNFA/1172] - BSA and Azide free (ab269772) | Abcam
Brand: Abcam Limited
SKU: AB269772

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(4 Publications)

Mouse Monoclonal TNF alpha antibody. Carrier free. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNF.

View Alternative Names

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] - BSA and Azide free (AB269772)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] - BSA and Azide free (AB269772)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human Erdheim Chester disease tissue labeling TNF alpha with ab220210 at 4 μg/mL.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220210)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody [TNFA/1172] - BSA and Azide free (AB269772)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat stomach tissue labeling TNF alpha with ab220210 at 4 μg/mL.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220210)

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TNFA/1172

Isotype

IgM

Light chain type

kappa

Carrier free

Yes

Reacts with

Rat, Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TNF.

P01375

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Product details

ab269772 is a carrier free version of ab220210.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed : 23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed : 16829952, PubMed : 22517918, PubMed : 23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed : 12794819). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

See full target information TNF

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:13264 PubMed40246912

2025

Immune response against antibiotic-resistant and antibiotic-sensitive staphylococcus aureus in a rat model of implant infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yingfang Fan,Amita Sekar,Madeline McCanne,Jean Yuh,Devika Dutta Kannambadi,Sashank Lekkala,Orhun K Muratoglu,Ebru Oral

Animal models and experimental medicine 7:145-155 PubMed38525803

2024

The efficacy of vitamin E in preventing arthrofibrosis after joint replacement.

Applications

Unspecified application

Species

Unspecified reactive species

Yingfang Fan,Jean Yuh,Sashank Lekkala,Mehmet D Asik,Andrew Thomson,Madeline McCanne,Mark A Randolph,Antonia F Chen,Ebru Oral

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 42:460-473 PubMed37609941

2023

The efficacy of antibiotic-eluting material in a two-stage model of periprosthetic joint infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yingfang Fan,Madeline McCanne,Jean Yuh,Sashank Lekkala,Charlotte P Leape,Shannon Hugard,Andrew Thomson,Jamie E Collins,Orhun K Muratoglu,Mark Randolph,Ebru Oral

Molecules (Basel, Switzerland) 28: PubMed36903410

2023

Effects of Oil on Sodium Hypochlorite-Induced Damage in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Güneş Bolatli,Fatih Taş,Naci Ömer Alayunt

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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