AB223352

Anti-TNF Receptor I antibody

Name: Anti-TNF Receptor I antibody (ab223352) | Abcam
Brand: Abcam Limited
SKU: AB223352
Price: 525 USD
Availability: InStock

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(17 Publications)

Anti-TNF Receptor I antibody (ab223352) is a rabbit polyclonal antibody detecting TNF Receptor I in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse.

View Alternative Names

CD120a, TNFAR, TNFR1, TNFRSF1A, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I

6 Images

Supplier Data

Western blot - Anti-TNF Receptor I antibody (AB223352)

Blocking : 5% skimmed milk in 1X TBST.

Primary incubation : 2 hours at room temperature

All lanes:

Western blot - Anti-TNF Receptor I antibody (ab223352) at 1/1000 dilution

All lanes:

mouse liver lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP at 1/2000 dilution

Predicted band size: 50 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor I antibody (AB223352)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor I antibody (AB223352)

2% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TNF Receptor I (green) using ab223352 at a dilution of 1/100 in ICC/IF (panel B). The secondary antibody is FITC Goat Anti-Rabbit used at 1/200 diliution. The nuclear counter stain is DAPI (blue)(panel A) used at 1/40,000 dilution. The merged images are shown in panel C.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor I antibody (AB223352)

Methanol-fixed HaCaT (human keratinocyte cell line) cells stained for TNF Receptor I (green) using ab223352 at a dilution of 1/100 in ICC/IF. The secondary antibody is FITC Goat Anti-Rabbit at a 1/50 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor I antibody (AB223352)

2% formaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TNF Receptor I (red) using ab223352 at a dilution of 1/100 in ICC/IF (panel B). The secondary antibody is APC Goat Anti-Rabbit used at 1/200 dilution. The nuclear counter stain is DAPI (blue)(panel A). The merged images are shown in panel C.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor I antibody (AB223352)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor I antibody (AB223352)

Sections of mouse back skin were stained for TNF Receptor I with ab223352 at a dilution of 1/100 in immunohistochemical analysis.

The secondary antibody was FITC Goat Anti-Rabbit (green) at a 1/50 dilution.

Supplier Data

Western blot - Anti-TNF Receptor I antibody (AB223352)

Blocking buffer : 5% BSA in TBST.

Other bands ( ~90-100kDa) can be explained by factors such as oligomerization, self-aggregation, cleavage of the TNFR1 extracellular domain, etc (Literature references : doi : 10.3389/fcell.2020.615141, 10.1128/MCB.22.8.2536-2543.2002, 10.1073/pnas.0307981100).

All lanes:

Western blot - Anti-TNF Receptor I antibody (ab223352) at 1/1000 dilution

All lanes:

Human A549 at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP at 1/4000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Mouse Tnfrsf1a aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P25118

Specificity

Detects ~55kDa. Other bands present may be the result of oligomerization, self-aggregation and/or cleavage of the TNF-R1 extracellular domain.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-TNF Receptor I antibody (ab223352) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of TNF Receptor I?
Anti-TNF Receptor I (ab223352) specifically detects a band for TNF Receptor I (UniProt: P19438) at a molecular weight of 50.5kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.

See full target information TNFRSF1A

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7309 PubMed40774959

2025

RIPK1 kinase drove brain microvascular endothelial cells death and blood-brain barrier disruption in neonatal Escherichia coli meningitis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuhang Wang,Yuhan Zhang,Xinru Chen,Kailai Fu,Jiaqi Cui,Jiaoling Wu,Yu Sun,Jianluan Ren,Feng Xue,Jianjun Dai,Fang Tang

World journal of surgical oncology 23:71 PubMed40025550

2025

EDN1 facilitates cisplatin resistance of non-small cell lung cancer cells by regulating the TNF signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhen,Long Sun,Li Ji,Shaochong Zhou,Yijin Cui,Zhenwei Li

Frontiers in physiology 15:1488951 PubMed39703667

2024

Spinal TNF-α receptor 1 is differentially required for phrenic long-term facilitation (pLTF) over the course of motor neuron death in adult rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan D Lewis,Amy N Keilholz,Catherine L Smith,Ethan A Burd,Nicole L Nichols

JOR spine 7:e70006 PubMed39391171

2024

Inactivation of Tnf-α/Tnfr signaling attenuates progression of intervertebral disc degeneration in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chu Tao,Sixiong Lin,Yujia Shi,Weiyuan Gong,Mingjue Chen,Jianglong Li,Peijun Zhang,Qing Yao,Dongyang Qian,Zemin Ling,Guozhi Xiao

Nature communications 15:5731 PubMed38977708

2024

Targeting the transmembrane cytokine co-receptor neuropilin-1 in distal tubules improves renal injury and fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yinzheng Li,Zheng Wang,Huzi Xu,Yu Hong,Mengxia Shi,Bin Hu,Xiuru Wang,Shulin Ma,Meng Wang,Chujin Cao,Han Zhu,Danni Hu,Chang Xu,Yanping Lin,Gang Xu,Ying Yao,Rui Zeng

Journal of dairy science 107:8722-8735 PubMed38876213

2024

Unveiling the regulatory role of SIRT1 in the oxidative stress response of bovine mammary cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yufei Zhang,Juxiong Liu,Shuai Yuan,Shu Liu,Meng Zhang,Huijie Hu,Yu Cao,Guiqiu Hu,Shoupeng Fu,Wenjin Guo

International journal of molecular sciences 24: PubMed38003573

2023

Lipids Reduce Cytokine-Induced Pro-Inflammatory Signalling and Barrier Dysfunction in Human Keratinocyte Models.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie M L Kok,Georgina C Dowd,Jaydee D Cabral,Lyn M Wise

Cell reports. Medicine 4:101228 PubMed37852176

2023

Replenishing decoy extracellular vesicles inhibits phenotype remodeling of tissue-resident cells in inflammation-driven arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Liang,Ke Wang,Xiaoyu Wei,Xiaoshan Gong,Hao Tang,Hao Xue,Jing Wang,Pengbin Yin,Licheng Zhang,Zaisong Ma,Ce Dou,Shiwu Dong,Jianzhong Xu,Fei Luo,Qinyu Ma

Small methods 7:e2300678 PubMed37526322

2023

Genetically Engineered Biomimetic Nanoparticles for Targeted Delivery of mRNA to Treat Rheumatoid Arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhai Chen,Jianwei Tan,Jian Li,Wenxiang Cheng,Liqing Ke,Anqiao Wang,Qiqing Wang,Sien Lin,Gang Li,Benguo Wang,Jingqin Chen,Peng Zhang

Journal of cellular physiology 238:2267-2281 PubMed37490340

2023

Macrophage autophagy contributes to immune liver injury in trichloroethylene sensitized mice: Critical role of TNF-α mediating mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Baiwang Ding,Sifan Zhou,Zhoujian Wang,Wei Liu,Lei Gao,Yani Ding,Hua Huang,Qixing Zhu,Jiaxiang Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TNF Receptor I antibody

Western blot - Anti-TNF Receptor I antibody (AB223352)

Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor I antibody (AB223352)

Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor I antibody (AB223352)

Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor I antibody (AB223352)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor I antibody (AB223352)

Western blot - Anti-TNF Receptor I antibody (AB223352)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (17)

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