AB8161

Anti-TNF Receptor II antibody [MR2-1]

Name: Anti-TNF Receptor II antibody [MR2-1] (ab8161) | Abcam
Brand: Abcam Limited
SKU: AB8161
Rating: 3 (3 reviews)

(3 Reviews)

(9 Publications)

Mouse Monoclonal TNF Receptor II antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TNFRSF1B.

View Alternative Names

CD120b, TNFBR, TNFR2, TNFRSF1B, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, TNF-R2, TNF-RII, TNFR-II

2 Images

Flow Cytometry - Anti-TNF Receptor II antibody [MR2-1] (AB8161)

Flow Cyt

Unknown

Flow Cytometry - Anti-TNF Receptor II antibody [MR2-1] (AB8161)

Overlay histogram showing HL60 cells stained with ab8161 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8161, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor II antibody [MR2-1] (AB8161)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor II antibody [MR2-1] (AB8161)

ICC/IF image of ab8161 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MR2-1

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TNFRSF1B. The exact immunogen used to generate this antibody is proprietary information.

P20333

Specificity

The antibody reacts with the extra-cellular part of the TNF-R II and with the soluble receptor.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Cynomolgus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

See full target information TNFRSF1B

style

left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic translation 36:132-144 PubMed36185580

2022

Triptolide attenuates inhibition of ankylosing spondylitis-derived mesenchymal stem cells on the osteoclastogenesis through modulating exosomal transfer of circ-0110634.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Ji,Yueyang Lu,Zhuoyi Ma,Ke Gan,Yan Liu,Yue Cheng,Junliang Xu,Shijia Liu,Yunke Guo,Shanhang Han,Zengyan Zhao,Hanmei Xu,Weiyan Qi

Frontiers in immunology 13:850240 PubMed35432372

2022

Breast Density and Estradiol Are Major Determinants for Soluble TNF-TNF-R Proteins in Human Breast Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Jimmy Ekstrand,Maja Zemmler,Annelie Abrahamsson,Peter Lundberg,Mikael Forsgren,Charlotta Dabrosin

The journal of headache and pain 22:31 PubMed33902429

2021

P2X receptor upregulation in trigeminal ganglion neurons through TNFα production in macrophages contributes to trigeminal neuropathic pain in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Momoko Koizumi,Sayaka Asano,Akihiko Furukawa,Yoshinori Hayashi,Suzuro Hitomi,Ikuko Shibuta,Katsuhiko Hayashi,Fusao Kato,Koichi Iwata,Masamichi Shinoda

BMC complementary medicine and therapies 20:1 PubMed32020859

2020

Cordyceps militaris induces apoptosis in ovarian cancer cells through TNF-α/TNFR1-mediated inhibition of NF-κB phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Eunbi Jo,Hyun-Jin Jang,Kyeong Eun Yang,Min Su Jang,Yang Hoon Huh,Hwa-Seung Yoo,Jun Soo Park,Ik-Soon Jang,Soo Jung Park

Molecular medicine reports 16:2962-2968 PubMed28677724

2017

TNFR2 promotes Adriamycin resistance in breast cancer cells by repairing DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Fuqian Yang,Nana Zhao,Nan Wu

Experimental cell research 317:791-801 PubMed21182837

2010

Tumor necrosis factor alpha promotes the expression of immunosuppressive proteins and enhances the cell growth in a human bone marrow-derived stem cell culture.

Applications

ICC/IF

Species

Human

Johanna A Miettinen,Mika Pietilä,Riikka J Salonen,Steffen Ohlmeier,Kari Ylitalo,Heikki V Huikuri,Petri Lehenkari

The Journal of biological chemistry 284:19331-9 PubMed19473970

2009

FOXO3a regulates oxygen-responsive expression of tumor necrosis factor receptor 2 in human dermal microvascular endothelial cells.

Applications

Species

Human

Bo Ding,Nancy C Kirkiles-Smith,Jordan S Pober

The Journal of biological chemistry 280:5994-6004 PubMed15572357

2004

The Met-196 -> Arg variation of human tumor necrosis factor receptor 2 (TNFR2) affects TNF-alpha-induced apoptosis by impaired NF-kappaB signaling and target gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Till,Philip Rosenstiel,Anja Krippner-Heidenreich,Silvia Mascheretti-Croucher,Peter J P Croucher,Heiner Schäfer,Peter Scheurich,Dirk Seegert,Stefan Schreiber

Journal of immunology (Baltimore, Md. : 1950) 152:4036-43 PubMed8144970

1994

Slow release of soluble TNF receptors by monocytes in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

J F Leeuwenberg,T M Jeunhomme,W A Buurman

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TNF Receptor II antibody [MR2-1]

Flow Cytometry - Anti-TNF Receptor II antibody [MR2-1] (AB8161)

Immunocytochemistry/ Immunofluorescence - Anti-TNF Receptor II antibody [MR2-1] (AB8161)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (9)

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise