Skip to main content

Knockout Tested Rabbit Recombinant Monoclonal TNFAIP3 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 35 publications.


Images

Western blot - Anti-TNFAIP3 antibody [EPR2663] (AB92324), expandable thumbnail
  • Western blot - Anti-TNFAIP3 antibody [EPR2663] (AB92324), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] (AB92324), expandable thumbnail
  • Western blot - Anti-TNFAIP3 antibody [EPR2663] (AB92324), expandable thumbnail
  • Western blot - Anti-TNFAIP3 antibody [EPR2663] (AB92324), expandable thumbnail

Publications

  • Archives of gerontology and geriatrics 117:1052742023
    Protective effect of TNFAIP3 on testosterone production in Leydig cells under an aging inflammatory microenvironment.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Dong Xing,Yihan Jin,Dalin Sun,Yuanyuan Liu,Bin Cai,Chao Gao,Yugui Cui,Baofang Jin
    PubMed 37995648
  • Stem cell research & therapy 14:2532023
    Human osteoarthritic articular cartilage stem cells suppress osteoclasts and improve subchondral bone remodeling in experimental knee osteoarthritis partially by releasing TNFAIP3.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Zhi-Ling Li,Xiao-Tong Li,Rui-Cong Hao,Fei-Yan Wang,Yu-Xing Wang,Zhi-Dong Zhao,Pei-Lin Li,Bo-Feng Yin,Ning Mao,Li Ding,Heng Zhu
    PubMed 37752608

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Not recommended
Not recommended
Tested
Mouse
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000 - 1/5000

Notes

-

Not recommended
Not recommended

Species

Human, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Mouse species is recommended based on WB results,we do not guarantee IHC-P for mouse.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Mouse species is recommended based on WB results,we do not guarantee IHC-P for mouse.

Associated Products

Select an associated product type

10 products for Alternative Product

    5 products for Alternative Version

      Target data

      Function

      Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages.

      Alternative names

      Tumor necrosis factor alpha-induced protein 3, TNF alpha-induced protein 3, OTU domain-containing protein 7C, Putative DNA-binding protein A20, Zinc finger protein A20, TNFAIP3, OTUD7C

      Recommended products

      1. Loading...
      2. Loading...
      3. Loading...
      4. Loading...

      Knockout Tested Rabbit Recombinant Monoclonal TNFAIP3 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 35 publications.

      Alternative names

      Tumor necrosis factor alpha-induced protein 3, TNF alpha-induced protein 3, OTU domain-containing protein 7C, Putative DNA-binding protein A20, Zinc finger protein A20, TNFAIP3, OTUD7C

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR2663

      Purification technique

      Affinity purification Protein A

      Specificity

      Mouse species is recommended based on WB results,we do not guarantee IHC-P for mouse.

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Stable for 12 months at -20°C

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      11 product images

      • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324), expandable thumbnail

        Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

        Lanes 1-4: Merged signal (red and green). Green - ab92324 observed at 80 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

        ab92324 Anti-TNFAIP3 antibody [EPR2663] was shown to specifically react with TNFAIP3 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human TNFAIP3 knockout HeLa cell line ab265983 (knockout cell lysate Human TNFAIP3 knockout HeLa cell lysate ab257112) was used. Wild-type and TNFAIP3 knockout samples were subjected to SDS-PAGE. ab92324 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

        All lanes: Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324) at 1/1000 dilution

        Lane 1: Wild-type HeLa cell lysate at 20 µg

        Lane 2: TNFAIP3 knockout HeLa cell lysate at 20 µg

        Lane 3: Jurkat cell treated with 5ng/ml PMA for 48 hours and then treated with 2µg/ml PHA for 48 hours, whole cell lysate at 20 µg

        Lane 4: Untreated Jurkat cell lysate at 20 µg

        Performed under reducing conditions.

        Predicted band size: 89 kDa

        Observed band size: 80 kDa

      • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324), expandable thumbnail

        Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

        Lanes 1- 4: Merged signal (red and green). Green - ab92324 observed at 90 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

        ab92324 was shown to react with TNFAIP3 in wild-type A549 cells in western blot. Loss of signal was observed when knockout cell line Human TNFAIP3 knockout A549 cell line ab266946 (knockout cell lysate Human TNFAIP3 knockout A549 cell lysate ab257114) was used. Wild-type A549 and TNFAIP3 knockout A549 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab92324 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

        All lanes: Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324) at 1/1000 dilution

        Lane 1: Wild-type A549 cell lysate at 20 µg

        Lane 2: TNFAIP3 knockout A549 cell lysate at 20 µg

        Lane 3: Wild-type HeLa cell lysate at 20 µg

        Lane 4: TNFAIP3 knockout HeLa cell lysate at 20 µg

        Performed under reducing conditions.

        Predicted band size: 89 kDa

        Observed band size: 90 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] (ab92324), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TNFAIP3 with purified ab92324 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

      • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324), expandable thumbnail

        Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

        All lanes: Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324) at 1/5000 dilution

        Lane 1: WEHI-3 (Mouse leukemia lymphoblast) whole cell lysate at 20 µg

        Lane 2: WEHI-3 treated with 20 ng/ml TNF alpha (Recombinant human TNF alpha protein ab9642) for 6 h at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

        Predicted band size: 89 kDa

        Observed band size: 80 kDa

      • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324), expandable thumbnail

        Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

        Lanes 1-4: Merged signal (red and green). Green - ab92324 observed at 80 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

        ab92324 Anti-TNFAIP3 antibody [EPR2663] was shown to specifically react with TNFAIP3 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human TNFAIP3 knockout HeLa cell line ab265983 (knockout cell lysate Human TNFAIP3 knockout HeLa cell lysate ab257112) was used. Wild-type and TNFAIP3 knockout samples were subjected to SDS-PAGE. ab92324 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

        All lanes: Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324) at 1/1000 dilution

        Lane 1: Wild-type HeLa cell lysate at 20 µg

        Lane 2: TNFAIP3 knockout HeLa cell lysate at 20 µg

        Lane 3: Jurkat cell treated with 5ng/ml PMA for 48 hours and then treated with 2µg/ml PHA for 48 hours, whole cell lysate at 20 µg

        Lane 4: Untreated Jurkat cell lysate at 20 µg

        Performed under reducing conditions.

        Predicted band size: 89 kDa

        Observed band size: 80 kDa

      • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324), expandable thumbnail

        Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

        Blocking and dilution buffer: 5% NFDM/TBST.

        All lanes: Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324) at 1/2000 dilution

        All lanes: Jurkat cell lysate - treated with TNF (Recombinant human TNF alpha protein ab9642) and TPA at 10 µg

        Secondary

        All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

        Predicted band size: 89 kDa

        Observed band size: 80 kDa

      • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324), expandable thumbnail

        Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

        Blocking and dilution buffer: 5% NFDM/TBST.

        All lanes: Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324) at 1/3000 dilution

        All lanes: Jurkat cell lysate - treated with TNF (Recombinant human TNF alpha protein ab9642) and TPA at 10 µg

        Secondary

        All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

        Predicted band size: 89 kDa

        Observed band size: 80 kDa

      • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324), expandable thumbnail

        Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

        Lanes 1-4: Merged signal (red and green). Green - ab92324 observed at 80 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

        ab92324 Anti-TNFAIP3 antibody [EPR2663] was shown to specifically react with TNFAIP3 in wild-type A549 cells. Loss of signal was observed when knockout cell line Human TNFAIP3 knockout A549 cell line ab266945 (knockout cell lysate Human TNFAIP3 knockout A549 cell lysate ab257113) was used. Wild-type and TNFAIP3 knockout samples were subjected to SDS-PAGE. ab92324 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

        All lanes: Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324) at 1/1000 dilution

        Lane 1: Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

        Lane 2: TNFAIP3 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

        Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) cell treated with 5ng/ml PMA for 48 hours and then treated with 2µg/ml PHA for 48 hours, whole cell lysate at 20 µg

        Lane 4: Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

        Predicted band size: 89 kDa

        Observed band size: 80 kDa

        This data was developed using ab92324, the same antibody clone in a different buffer formulation.

        Lanes 1-4: Merged signal (red and green). Green - ab92324 observed at 80 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

        ab92324 Anti-TNFAIP3 antibody [EPR2663] was shown to specifically react with TNFAIP3 in wild-type A549 cells. Loss of signal was observed when knockout cell line Human TNFAIP3 knockout A549 cell line ab266945 (knockout cell lysate Human TNFAIP3 knockout A549 cell lysate ab257113) was used. Wild-type and TNFAIP3 knockout samples were subjected to SDS-PAGE. ab92324 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

      • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324), expandable thumbnail

        Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

        All lanes: Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324) at 1/1000 dilution

        Lane 1: Jurkat cells treated with TNF (Recombinant human TNF alpha protein ab9642) and TPA at 10 µg

        Lane 2: Daudi cell lysate at 10 µg

        Secondary

        All lanes: HRP labelled goat anti-rabbit IgG at 1/2000 dilution

        Predicted band size: 89 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] (ab92324), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TNFAIP3 with unpurified ab92324 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

      • Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324), expandable thumbnail

        Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324)

        Western blot: Anti-TNFAIP3 antibody [EPR2663] (ab92324) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab92324 was shown to bind specifically to TNFAIP3. A band was observed at 90 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in TNFAIP3 knockout cell line. To generate this image, wild-type and TNFAIP3 knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

        All lanes: Western blot - Anti-TNFAIP3 antibody [EPR2663] (ab92324) at 1/1000 dilution

        Lane 1: Wild-type U-87 MG cell lysate at 20 µg

        Lane 2: TNFAIP3 knockout U-87 MG cell lysate at 20 µg

        Lane 3: Wild-type A549 cell lysate at 20 µg

        Lane 4: TNFAIP3 knockout A549 cell lysate at 20 µg

        Secondary

        All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

        Performed under reducing conditions.

      Downloads

      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

      For licensing inquiries, please contact partnerships@abcam.com