Knockout Tested Rabbit Recombinant Monoclonal TNFAIP3 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 9 publications.
Images
![Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (AB227987), expandable thumbnail](https://content.abcam.com/products/images/tnfaip3-antibody-epr2663-bsa-and-azide-free-ab227987--western-blot-img6548.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (AB227987)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (AB227987), expandable thumbnail](https://content.abcam.com/products/images/tnfaip3-antibody-epr2663-bsa-and-azide-free-ab227987--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34184.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (AB227987)")
![Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (AB227987), expandable thumbnail](https://content.abcam.com/products/images/tnfaip3-antibody-epr2663-bsa-and-azide-free-ab227987--western-blot-img358.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (AB227987)")
![Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (AB227987), expandable thumbnail](https://content.abcam.com/products/images/tnfaip3-antibody-epr2663-bsa-and-azide-free-ab227987--western-blot-img25.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (AB227987)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (AB227987), expandable thumbnail](https://content.abcam.com/products/images/tnfaip3-antibody-epr2663-bsa-and-azide-free-ab227987--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72627.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (AB227987)")
Publications
Filter by
- PloS one 12:e01690262017Two Antagonistic MALT1 Auto-Cleavage Mechanisms Reveal a Role for TRAF6 to Unleash MALT1 Activation.
- Journal of neuroinflammation 13:2582016Upregulation of neuronal zinc finger protein A20 expression is required for electroacupuncture to attenuate the cerebral inflammatory injury mediated by the nuclear factor-kB signaling pathway in cerebral ischemia/reperfusion rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Zhan et. al.PubMed 27716383
- Transgenic research 26:153-1632016Generation of α-1,3-galactosyltransferase knocked-out transgenic cloned pigs with knocked-in five human genes.Applications:WBReactive species:Unspecified reactive speciesDae-Jin Kwon,Dong-Hwan Kim,In-Sul Hwang,Dong-Ern Kim,Hyung-Joo Kim,Jang-Seong Kim,Kichoon Lee,Gi-Sun Im,Jeong-Woong Lee,Seongsoo HwangPubMed 27554374
- Medicine 94:e15012015Upregulated Expression of A20 on Monocytes is Associated With Increased Severity of Acute-on-Chronic Hepatitis B Liver Failure: A Case-Control Study.Applications:Flow CytReactive species:Unspecified reactive speciesYonghong Guo,Yu He,Ying Zhang,Yun Zhou,Yuan Qin,Chao Fan,Guangxi Ji,Peixin Zhang,Zhansheng JiaPubMed 26426612
- PloS one 10:e01239222015Frequent down regulation of the tumor suppressor gene a20 in multiple myeloma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatharina Troppan et. al.PubMed 25856582
- Genome biology 16:22015NF-κB-direct activation of microRNAs with repressive effects on monocyte-specific genes is critical for osteoclast differentiation.Applications:WBReactive species:Unspecified reactive speciesLorenzo de la Rica et. al.PubMed 25601191
- Journal of molecular histology 43:319-252012Expression of A20 is reduced in pancreatic cancer tissues.
- Investigative ophthalmology & visual science 52:8442-542011Neurodegenerative and inflammatory pathway components linked to TNF-α/TNFR1 signaling in the glaucomatous human retina.
- Blood 117:4852-42011A20 (TNFAIP3) genetic alterations in EBV-associated AIDS-related lymphoma.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Tested | Not recommended | Not recommended | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Mouse species is recommended based on WB results,we do not guarantee IHC-P for mouse. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
6 products for Alternative Version
Target data
Function
Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages.
Alternative names
Tumor necrosis factor alpha-induced protein 3, TNF alpha-induced protein 3, OTU domain-containing protein 7C, Putative DNA-binding protein A20, Zinc finger protein A20, TNFAIP3, OTUD7C
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal TNFAIP3 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 9 publications.
Alternative names
Tumor necrosis factor alpha-induced protein 3, TNF alpha-induced protein 3, OTU domain-containing protein 7C, Putative DNA-binding protein A20, Zinc finger protein A20, TNFAIP3, OTUD7C
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR2663
- Purification technique
Affinity purification Protein A
- Specificity
Mouse species is recommended based on WB results,we do not guarantee IHC-P for mouse.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab227987 is the carrier-free version of Anti-TNFAIP3 antibody [EPR2663] ab92324.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987), expandable thumbnail](https://content.abcam.com/products/images/tnfaip3-antibody-epr2663-bsa-and-azide-free-ab227987--western-blot-img6548.jpg "Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987)")
Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987)
This data was developed using the same antibody clone in a different buffer formulation (Anti-TNFAIP3 antibody [EPR2663] ab92324).
Lanes 1- 4: Merged signal (red and green). Green - Anti-TNFAIP3 antibody [EPR2663] ab92324 observed at 90 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-TNFAIP3 antibody [EPR2663] ab92324 was shown to react with TNFAIP3 in wild-type A549 cells in western blot. Loss of signal was observed when knockout cell line Human TNFAIP3 knockout A549 cell line ab266946 (knockout cell lysate Human TNFAIP3 knockout A549 cell lysate ab257114) was used. Wild-type A549 and TNFAIP3 knockout A549 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-TNFAIP3 antibody [EPR2663] ab92324 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-TNFAIP3 antibody [EPR2663] (Anti-TNFAIP3 antibody [EPR2663] ab92324) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: TNFAIP3 knockout A549 cell lysate at 20 µg
Lane 3: Wild-type HeLa cell lysate at 20 µg
Lane 4: TNFAIP3 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 89 kDa
Observed band size: 90 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987), expandable thumbnail](https://content.abcam.com/products/images/tnfaip3-antibody-epr2663-bsa-and-azide-free-ab227987--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34184.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TNFAIP3 with purified Anti-TNFAIP3 antibody [EPR2663] ab92324 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TNFAIP3 antibody [EPR2663] ab92324).
![Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987), expandable thumbnail](https://content.abcam.com/products/images/tnfaip3-antibody-epr2663-bsa-and-azide-free-ab227987--western-blot-img358.jpg "Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987)")
Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987)
This data was developed using the same antibody clone in a different buffer formulation (Anti-TNFAIP3 antibody [EPR2663] ab92324).
Lanes 1-4: Merged signal (red and green). Green - Anti-TNFAIP3 antibody [EPR2663] ab92324 observed at 80 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-TNFAIP3 antibody [EPR2663] ab92324 Anti-TNFAIP3 antibody [EPR2663] was shown to specifically react with TNFAIP3 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human TNFAIP3 knockout HeLa cell line ab265983 (knockout cell lysate Human TNFAIP3 knockout HeLa cell lysate ab257112) was used. Wild-type and TNFAIP3 knockout samples were subjected to SDS-PAGE. Anti-TNFAIP3 antibody [EPR2663] ab92324 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-TNFAIP3 antibody [EPR2663] (Anti-TNFAIP3 antibody [EPR2663] ab92324) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: TNFAIP3 knockout HeLa cell lysate at 20 µg
Lane 3: Jurkat cell treated with 5ng/ml PMA for 48 hours and then treated with 2µg/ml PHA for 48 hours, whole cell lysate at 20 µg
Lane 4: Untreated Jurkat cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 89 kDa
Observed band size: 80 kDa
![Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987), expandable thumbnail](https://content.abcam.com/products/images/tnfaip3-antibody-epr2663-bsa-and-azide-free-ab227987--western-blot-img25.jpg "Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987)")
Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987)
This data was developed using Anti-TNFAIP3 antibody [EPR2663] ab92324, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - Anti-TNFAIP3 antibody [EPR2663] ab92324 observed at 80 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
Anti-TNFAIP3 antibody [EPR2663] ab92324 Anti-TNFAIP3 antibody [EPR2663] was shown to specifically react with TNFAIP3 in wild-type A549 cells. Loss of signal was observed when knockout cell line Human TNFAIP3 knockout A549 cell line ab266945 (knockout cell lysate Human TNFAIP3 knockout A549 cell lysate ab257113) was used. Wild-type and TNFAIP3 knockout samples were subjected to SDS-PAGE. Anti-TNFAIP3 antibody [EPR2663] ab92324 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-TNFAIP3 antibody [EPR2663] (Anti-TNFAIP3 antibody [EPR2663] ab92324) at 1/1000 dilution
Lane 1: Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 2: TNFAIP3 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) cell treated with 5ng/ml PMA for 48 hours and then treated with 2µg/ml PHA for 48 hours, whole cell lysate at 20 µg
Lane 4: Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 89 kDa
Observed band size: 80 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987), expandable thumbnail](https://content.abcam.com/products/images/tnfaip3-antibody-epr2663-bsa-and-azide-free-ab227987--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72627.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987)
This IHC data was generated using the same anti-TNFAIP3 antibody clone, EPR2663, in a different buffer formulation (cat# Anti-TNFAIP3 antibody [EPR2663] ab92324).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TNFAIP3 with unpurified Anti-TNFAIP3 antibody [EPR2663] ab92324 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
![Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987), expandable thumbnail](https://content.abcam.com/products/images/tnfaip3-antibody-epr2663-bsa-and-azide-free-ab227987--western-blot-img432518.jpg "Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987)")
Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987)
Western blot: Anti-TNFAIP3 antibody [EPR2663] (Anti-TNFAIP3 antibody [EPR2663] ab92324) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-TNFAIP3 antibody [EPR2663] ab92324 was shown to bind specifically to TNFAIP3. A band was observed at 90 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in TNFAIP3 knockout cell line. To generate this image, wild-type and TNFAIP3 knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-TNFAIP3 antibody [EPR2663] (Anti-TNFAIP3 antibody [EPR2663] ab92324) at 1/1000 dilution
Lane 1: Wild-type U-87 MG cell lysate at 20 µg
Lane 2: TNFAIP3 knockout U-87 MG cell lysate at 20 µg
Lane 3: Wild-type A549 cell lysate at 20 µg
Lane 4: TNFAIP3 knockout A549 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
![Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987), expandable thumbnail](https://content.abcam.com/products/images/tnfaip3-antibody-epr2663-bsa-and-azide-free-ab227987--western-blot-img432518.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TNFAIP3 antibody [EPR2663] - BSA and Azide free (ab227987)")
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