AB195810

Anti-TNFAIP8 antibody [EPR10058(3)]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Recombinant Monoclonal TNFAIP8 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

Tumor necrosis factor alpha-induced protein 8, TNF alpha-induced protein 8, Head and neck tumor and metastasis-related protein, MDC-3.13, NF-kappa-B-inducible DED-containing protein, SCC-S2, TNF-induced protein GG2-1, NDED, TNFAIP8

3 Images

Supplier Data

Immunoprecipitation - Anti-TNFAIP8 antibody [EPR10058(3)] (AB195810)

TNFAIP8 was immunoprecipitated from K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab195810 at 1/70 dilution (Lane 2). Western blot was performed from the immunoprecipitate using ab195810 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1 : K562 whole cell extract (Input) 10μg. Lane 2 : ab195810 IP in K562 whole cell extract. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab195810 in K562 whole cell extract.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810)

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (AB195810)

Blocking/Dilution buffer : 5% NFDM/TBST.

Based on the sequence analysis, ab195810 recognizes three isoforms with the predicted MWs of 23KDa, 22KDa and 22KDa, respectively.

All lanes:

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810) at 1/1000 dilution

All lanes:

Human fetal spleen lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 19 kDa,21 kDa

false

Supplier Data

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (AB195810)

Blocking/Dilution buffer : 5% NFDM/TBST.

Based on the sequence analysis, ab195810 recognizes three isoforms with the predicted MWs of 23KDa, 22KDa and 22KDa, respectively.

All lanes:

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810) at 1/1000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 19 kDa,21 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10058(3)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/70", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Acts as a negative mediator of apoptosis and may play a role in tumor progression. Suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation.

See full target information Tumor necrosis factor alpha-induced protein 8

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of genomics 2021:3997045 PubMed34901263

2021

circHUWE1 Exerts an Oncogenic Role in Inducing DDP-Resistant NSCLC Progression Depending on the Regulation of miR-34a-5p/TNFAIP8.

Applications

Unspecified application

Species

Unspecified reactive species

Xueliang Yang,Quan Sun,Yongming Song,Wenli Li

Cancer research 82:221-234 PubMed34625422

2021

Novel Oncogenic Transcription Factor Cooperation in RB-Deficient Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Amy C Mandigo,Ayesha A Shafi,Jennifer J McCann,Wei Yuan,Talya S Laufer,Denisa Bogdan,Lewis Gallagher,Emanuela Dylgjeri,Galina Semenova,Irina A Vasilevskaya,Matthew J Schiewer,Chris M McNair,Johann S de Bono,Karen E Knudsen

Endocrine connections 10:471-483 PubMed33764889

2021

Long noncoding RNA DLEU2 drives the malignant behaviors of thyroid cancer through mediating the miR-205-5p/TNFAIP8 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwen Yang,Yayin Huang,Bohan Dong,Yunhai Dai

Oncology letters 21:118 PubMed33376549

2020

Expression of TIPE family members in human colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mengya Zhong,Zhijian Chen,Yang Yan,Argen Bahet,Xin Cai,Huiyu Chen,Honggang Ran,Kaiyong Qu,Zhaopu Han,Guohong Zhuang,Shifeng Zhang,Yinan Wang

Journal of Cancer 11:3061-3071 PubMed32226521

2020

TNFAIP8 promotes the migration of clear cell renal cell carcinoma by regulating the EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Mengya Zhong,Maoshu Zhu,Yu Liu,Ying Lin,Lianghai Wang,Yuhan Ye,Huiyu Chen,Yan Yang,Guohong Zhuang,Jiyi Huang

Oncology letters 17:4667-4674 PubMed30944654

2019

TNFAIP8 promotes cisplatin resistance in cervical carcinoma cells by inhibiting cellular apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Suxia Wu,Weihua Li,Zhenghui Wu,Tianran Cheng,Ping Wang,Na Li,Xiaonan Liang,Mengmeng Chi,Shuman Zhang,Yuanfang Ma,Yanyun Li,Lihui Chai

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TNFAIP8 antibody [EPR10058(3)]

Immunoprecipitation - Anti-TNFAIP8 antibody [EPR10058(3)] (AB195810)

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (AB195810)

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (AB195810)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise