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Rabbit Recombinant Monoclonal TNFAIP8 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications.

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Images

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (AB195810), expandable thumbnail
  • Immunoprecipitation - Anti-TNFAIP8 antibody [EPR10058(3)] (AB195810), expandable thumbnail
  • Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (AB195810), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/70
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

Acts as a negative mediator of apoptosis and may play a role in tumor progression. Suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation.

Alternative names

Tumor necrosis factor alpha-induced protein 8, TNF alpha-induced protein 8, Head and neck tumor and metastasis-related protein, MDC-3.13, NF-kappa-B-inducible DED-containing protein, SCC-S2, TNF-induced protein GG2-1, NDED, TNFAIP8

Recommended products

Rabbit Recombinant Monoclonal TNFAIP8 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10058(3)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810), expandable thumbnail

    Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Based on the sequence analysis, ab195810 recognizes three isoforms with the predicted MWs of 23KDa, 22KDa and 22KDa, respectively.

    All lanes: Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810) at 1/1000 dilution

    Lane 1: K562 cell lysate at 20 µg

    Lane 2: A549 cell lysate at 20 µg

    Lane 3: A431 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa

    Observed band size: 19 kDa, 21 kDa

  • Immunoprecipitation - Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810), expandable thumbnail

    Immunoprecipitation - Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810)

    TNFAIP8 was immunoprecipitated from K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab195810 at 1/70 dilution (Lane 2). Western blot was performed from the immunoprecipitate using ab195810 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: K562 whole cell extract (Input) 10μg. Lane 2: ab195810 IP in K562 whole cell extract. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab195810 in K562 whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810)

    Predicted band size: 23 kDa

  • Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810), expandable thumbnail

    Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Based on the sequence analysis, ab195810 recognizes three isoforms with the predicted MWs of 23KDa, 22KDa and 22KDa, respectively.

    All lanes: Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810) at 1/1000 dilution

    All lanes: Human fetal spleen lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 23 kDa

    Observed band size: 19 kDa, 21 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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