Anti-TNFAIP8 antibody [EPR10058(3)] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-TNFAIP8 antibody [EPR10058(3)] - BSA and Azide free (AB251212)

This data was developed using ab195810, the same antibody clone in a different buffer formulation.

TNFAIP8 was immunoprecipitated from K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab195810 at 1/70 dilution (Lane 2). Western blot was performed from the immunoprecipitate using ab195810 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1 : K562 whole cell extract (Input) 10μg. Lane 2 : ab195810 IP in K562 whole cell extract. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab195810 in K562 whole cell extract.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-TNFAIP8 antibody [EPR10058(3)] (<a href='/en-us/products/primary-antibodies/tnfaip8-antibody-epr100583-ab195810'>ab195810</a>)

Predicted band size: 23 kDa

false

• WB

Supplier Data

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] - BSA and Azide free (AB251212)

This data was developed using ab195810, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Based on the sequence analysis, ab195810 recognizes three isoforms with the predicted MWs of 23KDa, 22KDa and 22KDa, respectively.

All lanes:

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (<a href='/en-us/products/primary-antibodies/tnfaip8-antibody-epr100583-ab195810'>ab195810</a>) at 1/1000 dilution

All lanes:

Human fetal spleen lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 19 kDa,21 kDa

false

• WB

Supplier Data

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] - BSA and Azide free (AB251212)

This data was developed using ab195810, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Based on the sequence analysis, ab195810 recognizes three isoforms with the predicted MWs of 23KDa, 22KDa and 22KDa, respectively.

All lanes:

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (<a href='/en-us/products/primary-antibodies/tnfaip8-antibody-epr100583-ab195810'>ab195810</a>) at 1/1000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 19 kDa,21 kDa

false