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AB323371

Anti-TNFRSF10B antibody [RM2078]

  • KO Validated
  • Recombinant
  • 20ul selling size
  • RabMAb
  • What is this?

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Rabbit Recombinant Multiclonal DR5 antibody. Suitable for WB and reacts with Human, Mouse samples.

View Alternative Names

CD262, Dr5, Killer, Tnfrsf10b, Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, MK

2 Images
Western blot - Anti-TNFRSF10B antibody [RM2078] (AB323371)
  • WB

Supplier Data

Western blot - Anti-TNFRSF10B antibody [RM2078] (AB323371)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab323371 was shown to bind specifically to TNFRSF10B. Target of interest was observed at 40, 48 kDa in wild-type HeLa cell lysates (lane 1) with no signal observed at this size in TNFRSF10B knockout cell line (lane 2) (lane 2, knockout cell line ab264922).

The lanes 1-5 were developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

Doxorubicin treatment elevated the expression of DR5 (PMID : 12496481; PMID : 11468181; PMID : 11090076).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane1-4 : 103 seconds; lane 5-6 : 59 seconds

All lanes:

Western blot - Anti-TNFRSF10B antibody [RM2078] (ab323371) at 1/1000 dilution

Lane 1:

Parental HeLa (human cervical adenocarcinoma epithelial cell) whole lysate at 20 µg

Lane 2:

TNFRSF10B knockout HeLa whole lysate at 20 µg

Lane 3:

HT-1080 (human fibrosarcoma epithelial cell) whole lysate at 20 µg

Lane 4:

HepG2 (human hepatocelullar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Untreated HCT 116 (human colorectal carcinoma epithelial cell) whole lysate at 20 µg

Lane 6:

HCT 116 treated with 0.5uM doxorubicin for 24 hours whole lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa,48 kDa,36 kDa

true

Western blot - Anti-TNFRSF10B antibody [RM2078] (AB323371)
  • WB

Supplier Data

Western blot - Anti-TNFRSF10B antibody [RM2078] (AB323371)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Neuro-2a

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TNFRSF10B antibody [RM2078] (ab323371) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

NIH/3T3 transfected with siRNA specifically targeting DR5 whole cell lysate at 20 µg

Lane 3:

C2C12 (mouse myoblast) whole cell lysate at 20 µg

Lane 4:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 5:

MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 48 kDa,36 kDa

false

Exposure time: 59s

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM2078

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB46013

Human DR5 ELISA Kit (CD262)

Sandwich ELISA - Human DR5 ELISA Kit (CD262) (AB46013)

  • 0
  • 0
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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
See full target information Tnfrsf10b
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com