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Rabbit Recombinant Multiclonal DR5 antibody. Suitable for WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-TNFRSF10B antibody [RM2078] (AB323371), expandable thumbnail
  • Western blot - Anti-TNFRSF10B antibody [RM2078] (AB323371), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-PIPFlow Cyt (Intra)
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info
1/1000
Notes

-

Species

Mouse

Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.

Alternative names

CD262, Dr5, Killer, Tnfrsf10b, Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, MK

Rabbit Recombinant Multiclonal DR5 antibody. Suitable for WB and reacts with Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

RM2078

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-TNFRSF10B antibody [RM2078] (ab323371), expandable thumbnail

    Western blot - Anti-TNFRSF10B antibody [RM2078] (ab323371)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: Neuro-2a

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-TNFRSF10B antibody [RM2078] (ab323371) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: NIH/3T3 transfected with siRNA specifically targeting DR5 whole cell lysate at 20 µg

    Lane 3: C2C12 (mouse myoblast) whole cell lysate at 20 µg

    Lane 4: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 5: MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 48 kDa, 36 kDa

    Exposure time: 59s

  • Western blot - Anti-TNFRSF10B antibody [RM2078] (ab323371), expandable thumbnail

    Western blot - Anti-TNFRSF10B antibody [RM2078] (ab323371)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, ab323371 was shown to bind specifically to TNFRSF10B. Target of interest was observed at 40, 48 kDa in wild-type HeLa cell lysates (lane 1) with no signal observed at this size in TNFRSF10B knockout cell line (lane 2) (lane 2, knockout cell line Human TNFRSF10B (DR5) knockout HeLa cell line ab264922).

    The lanes 1-5 were developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

    Doxorubicin treatment elevated the expression of DR5 (PMID: 12496481; PMID: 11468181; PMID: 11090076).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lane1-4: 103 seconds; lane 5-6: 59 seconds

    All lanes: Western blot - Anti-TNFRSF10B antibody [RM2078] (ab323371) at 1/1000 dilution

    Lane 1: Parental HeLa (human cervical adenocarcinoma epithelial cell) whole lysate at 20 µg

    Lane 2: TNFRSF10B knockout HeLa whole lysate at 20 µg

    Lane 3: HT-1080 (human fibrosarcoma epithelial cell) whole lysate at 20 µg

    Lane 4: HepG2 (human hepatocelullar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: Untreated HCT 116 (human colorectal carcinoma epithelial cell) whole lysate at 20 µg

    Lane 6: HCT 116 treated with 0.5uM doxorubicin for 24 hours whole lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 40 kDa, 48 kDa, 36 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com