Rabbit Recombinant Monoclonal TNFRSF14/HVEM antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.
Images
![Flow Cytometry - Anti-TNFRSF14/HVEM antibody [EPR22122] (AB224708), expandable thumbnail](https://content.abcam.com/products/images/tnfrsf14-hvem-antibody-epr22122-ab224708--flow-cytometry-img25245.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-TNFRSF14/HVEM antibody [EPR22122] (AB224708)")
![Flow Cytometry - Anti-TNFRSF14/HVEM antibody [EPR22122] (AB224708), expandable thumbnail](https://content.abcam.com/products/images/tnfrsf14-hvem-antibody-epr22122-ab224708--flow-cytometry-img37634.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-TNFRSF14/HVEM antibody [EPR22122] (AB224708)")
![Immunocytochemistry/ Immunofluorescence - Anti-TNFRSF14/HVEM antibody [EPR22122] (AB224708), expandable thumbnail](https://content.abcam.com/products/images/tnfrsf14-hvem-antibody-epr22122-ab224708--immunocytochemistry-immunofluorescence-img85630.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TNFRSF14/HVEM antibody [EPR22122] (AB224708)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/400 | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Target data
Function
Receptor for four distinct ligands: The TNF superfamily members TNFSF14/LIGHT and homotrimeric LTA/lymphotoxin-alpha and the immunoglobulin superfamily members BTLA and CD160, altogether defining a complex stimulatory and inhibitory signaling network (PubMed:9462508, PubMed:10754304, PubMed:18193050, PubMed:23761635). Signals via the TRAF2-TRAF3 E3 ligase pathway to promote immune cell survival and differentiation (PubMed:19915044, PubMed:9153189, PubMed:9162022). Participates in bidirectional cell-cell contact signaling between antigen presenting cells and lymphocytes. In response to ligation of TNFSF14/LIGHT, delivers costimulatory signals to T cells, promoting cell proliferation and effector functions (PubMed:10754304). Interacts with CD160 on NK cells, enhancing IFNG production and anti-tumor immune response (PubMed:23761635). In the context of bacterial infection, acts as a signaling receptor on epithelial cells for CD160 from intraepithelial lymphocytes, triggering the production of antimicrobial proteins and proinflammatory cytokines (By similarity). Upon binding to CD160 on activated CD4+ T cells, downregulates CD28 costimulatory signaling, restricting memory and alloantigen-specific immune response (PubMed:18193050). May interact in cis (on the same cell) or in trans (on other cells) with BTLA (PubMed:19915044) (By similarity). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed:19915044) (By similarity).(Microbial infection) Acts as a receptor for Herpes simplex virus 1/HHV-1.(Microbial infection) Acts as a receptor for Herpes simplex virus 2/HHV-2.
Alternative names
Tumor necrosis factor receptor superfamily member 14, Herpes virus entry mediator A, Tumor necrosis factor receptor-like 2, Herpesvirus entry mediator A, HveA, TR2, UNQ329/PRO509, TNFRSF14, HVEA, HVEM
Recommended products
Rabbit Recombinant Monoclonal TNFRSF14/HVEM antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.
Alternative names
Tumor necrosis factor receptor superfamily member 14, Herpes virus entry mediator A, Tumor necrosis factor receptor-like 2, Herpesvirus entry mediator A, HveA, TR2, UNQ329/PRO509, TNFRSF14, HVEA, HVEM
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR22122
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-TNFRSF14/HVEM antibody [EPR22122] (ab224708), expandable thumbnail](https://content.abcam.com/products/images/tnfrsf14-hvem-antibody-epr22122-ab224708--flow-cytometry-img25245.jpg "Flow Cytometry - Anti-TNFRSF14/HVEM antibody [EPR22122] (ab224708)")
Flow Cytometry - Anti-TNFRSF14/HVEM antibody [EPR22122] (ab224708)
Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with with a Myc-tagged human TNFRSF14/HVEM expression construct labeling TNFRSF14/HVEM with ab224708 at 1/400 dilution (red) compared with a rabbit monoclonal IgG Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
![Flow Cytometry - Anti-TNFRSF14/HVEM antibody [EPR22122] (ab224708), expandable thumbnail](https://content.abcam.com/products/images/tnfrsf14-hvem-antibody-epr22122-ab224708--flow-cytometry-img37634.jpg "Flow Cytometry - Anti-TNFRSF14/HVEM antibody [EPR22122] (ab224708)")
Flow Cytometry - Anti-TNFRSF14/HVEM antibody [EPR22122] (ab224708)
Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) labeling TNFRSF14/HVEM with ab224708 at 1/400 dilution (red) compared with a rabbit monoclonal IgG Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081), at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
![Immunocytochemistry/ Immunofluorescence - Anti-TNFRSF14/HVEM antibody [EPR22122] (ab224708), expandable thumbnail](https://content.abcam.com/products/images/tnfrsf14-hvem-antibody-epr22122-ab224708--immunocytochemistry-immunofluorescence-img85630.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TNFRSF14/HVEM antibody [EPR22122] (ab224708)")
Immunocytochemistry/ Immunofluorescence - Anti-TNFRSF14/HVEM antibody [EPR22122] (ab224708)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with with a Myc-tagged human TNFRSF14/HVEM expression construct labeling TNFRSF14/HVEM with ab224708 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in 293T cells transfected with a Myc-tagged human TNFRSF14/HVEM expression construct. The nuclear counter stain is DAPI (blue). Cells are counter stained for Myc using an Anti-Myc tag antibody [9E10] (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-Myc tag antibody [9E10] ab223894) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
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