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Rabbit Recombinant Monoclonal TNFRSF14/HVEM antibody. Suitable for WB, IHC-P and reacts with Human, Transfected cell line samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (AB314494), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (AB314494), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (AB314494), expandable thumbnail
  • Western blot - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (AB314494), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (AB314494), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow CytIP
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell line
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Transfected cell line
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Transfected cell line
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell line
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell line
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell line
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Receptor for four distinct ligands: The TNF superfamily members TNFSF14/LIGHT and homotrimeric LTA/lymphotoxin-alpha and the immunoglobulin superfamily members BTLA and CD160, altogether defining a complex stimulatory and inhibitory signaling network (PubMed:10754304, PubMed:18193050, PubMed:23761635, PubMed:9462508). Signals via the TRAF2-TRAF3 E3 ligase pathway to promote immune cell survival and differentiation (PubMed:19915044, PubMed:9153189, PubMed:9162022). Participates in bidirectional cell-cell contact signaling between antigen presenting cells and lymphocytes. In response to ligation of TNFSF14/LIGHT, delivers costimulatory signals to T cells, promoting cell proliferation and effector functions (PubMed:10754304). Interacts with CD160 on NK cells, enhancing IFNG production and anti-tumor immune response (PubMed:23761635). In the context of bacterial infection, acts as a signaling receptor on epithelial cells for CD160 from intraepithelial lymphocytes, triggering the production of antimicrobial proteins and pro-inflammatory cytokines (By similarity). Upon binding to CD160 on activated CD4+ T cells, down-regulates CD28 costimulatory signaling, restricting memory and alloantigen-specific immune response (PubMed:18193050). May interact in cis (on the same cell) or in trans (on other cells) with BTLA (By similarity) (PubMed:19915044). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (By similarity) (PubMed:19915044). (Microbial infection) Acts as a receptor for Herpes simplex virus 1/HHV-1. (Microbial infection) Acts as a receptor for Herpes simplex virus 2/HHV-2.

Alternative names

CD270, HVEA, HVEM, UNQ329/PRO509, TNFRSF14, Tumor necrosis factor receptor superfamily member 14, Herpes virus entry mediator A, Tumor necrosis factor receptor-like 2, Herpesvirus entry mediator A, HveA, TR2

Recommended products

Rabbit Recombinant Monoclonal TNFRSF14/HVEM antibody. Suitable for WB, IHC-P and reacts with Human, Transfected cell line samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28154-164
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494)

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a TNFRSF14 expression vector containing a Myc-his tag cell pellet; (B) HEK-293T transfected with an empty vector containing a Myc-his tag cell pellet. tissue labeling TNFRSF14/HVEM with ab314494 at 1/1000 (0.525 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on (A) HEK-293T transfected with a TNFRSF14 expression vector containing a Myc-his tag cell pellet; no staining on (B) HEK-293T transfected with an empty vector containing a Myc-his tag cell pellet. The section was incubated with ab314494 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling TNFRSF14/HVEM with ab314494 at 1/1000 (0.525 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on human cerebrum (PMID: 30987862). The section was incubated with ab314494 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494)

    Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling TNFRSF14/HVEM with ab314494 at 1/1000 (0.525 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human prostatic hyperplasia. The section was incubated with ab314494 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494), expandable thumbnail

    Western blot - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: cerebellum.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
    Exposure time: Lane 1: 37 seconds, lanes 2-3: 59 seconds.

    All lanes: Western blot - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494) at 1/1000 dilution

    Lane 1: Human tonsil tissue lysate at 50 µg

    Lane 2: Human thymus tissue lysate at 50 µg

    Lane 3: Human cerebellum tissue lysate at 50 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 30 kDa, 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling TNFRSF14/HVEM with ab314494 at 1/1000 (0.525 ug/ml) followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human tonsil (PMID: 30918139). The section was incubated with ab314494 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494), expandable thumbnail

    Western blot - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    TNFRSF14/HVEM is a glycoprotein of approximately 30 kDa and 50 kDa and detected as a 20 kDa and 40 kDa band after treated with Protein Deglycosylation MIX II.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494) at 1/1000 dilution

    Lane 1: Untreated SK-MEL-28 (human malignant melanoma) whole cell lysate at 50 µg

    Lane 2: SK-MEL-28 treated with Protein Deglycosylation Mix II at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 20, 30, 40, 50 kDa, 30 kDa, 40 kDa, 50 kDa

    Exposure time: 92s

  • Western blot - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494), expandable thumbnail

    Western blot - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: NCI-H1975 (PMID: 30429008), A549 (PMID: 30429008).
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-TNFRSF14/HVEM antibody [EPR28154-164] (ab314494) at 1/1000 dilution

    Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate at 50 µg

    Lane 2: SK-MEL-28 (human malignant melanoma) whole cell lysate at 50 µg

    Lane 3: NCI-H1975 (human adenocarcinoma lu epithelial cell) whole cell lysate at 50 µg

    Lane 4: A549 (human lu carcinoma epithelial cell) whole cell lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 30 kDa, 50 kDa

    Exposure time: 37s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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