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AB281907

Anti-TNFRSF14/HVEM antibody [HMHV-1B18] - Rabbit IgG (Chimeric)

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Rabbit Recombinant Monoclonal TNFRSF14/HVEM antibody. Suitable for ICC/IF, WB and reacts with Mouse samples.
2 Images
Immunocytochemistry/ Immunofluorescence - Anti-TNFRSF14/HVEM antibody [HMHV-1B18] - Rabbit IgG (Chimeric) (AB281907)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TNFRSF14/HVEM antibody [HMHV-1B18] - Rabbit IgG (Chimeric) (AB281907)

Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labelling TNFRSF14/HVEM with ab281907 at 10 μg/ml for 1 hour, followed by Alexa Fluor® 488 secondary antibody at 2 μg/ml (green/top-left). Image showing membrane staining. The nuclear counter stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody. RAW264.7 cells were fixed Shi-fix™ coverslips.

Western blot - Anti-TNFRSF14/HVEM antibody [HMHV-1B18] - Rabbit IgG (Chimeric) (AB281907)
  • WB

Supplier Data

Western blot - Anti-TNFRSF14/HVEM antibody [HMHV-1B18] - Rabbit IgG (Chimeric) (AB281907)

A primary incubation of 1h was used and protein was detected by chemiluminescence.

Lanes 1 - 3:

Western blot - Anti-TNFRSF14/HVEM antibody [HMHV-1B18] - Rabbit IgG (Chimeric) (ab281907) at 0.01 µg/mL

Lanes 1 - 3:

Western blot - Anti-TNFRSF14/HVEM antibody [HMHV-1B18] - Hamster IgG (Chimeric) (<a href='/en-us/products/primary-antibodies/tnfrsf14-hvem-antibody-hmhv-1b18-hamster-igg-chimeric-ab281906'>ab281906</a>) at 0.01 µg/mL

Lane 1:

Mouse spleen tissue lysate at 35 µg

Lane 2:

Mouse thymus tissue lysate at 35 µg

Lane 3:

Mouse lung tissue lysate at 35 µg

Secondary

All lanes:

Anti-rabbit secondary antibody

Predicted band size: 30 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HMHV-1B18

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Mouse

Applications

WB, ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

This rabbit monoclonal chimeric antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Proclin 300 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information TNFRSF14/HVEM
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com