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AB251524

Anti-TNFSF18/GITRL antibody [EPR20583] - BSA and Azide free

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Rabbit Recombinant Monoclonal TNFSF18/GITRL antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

AITRL, GITRL, TL6, UNQ149/PRO175, TNFSF18, Tumor necrosis factor ligand superfamily member 18, Activation-inducible TNF-related ligand, Glucocorticoid-induced TNF-related ligand, hGITRL

1 Images
Western blot - Anti-TNFSF18/GITRL antibody [EPR20583] - BSA and Azide free (AB251524)
  • WB

Supplier Data

Western blot - Anti-TNFSF18/GITRL antibody [EPR20583] - BSA and Azide free (AB251524)

This data was developed using ab211037, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

TNFSF18 is constitutively expressed in HUVEC cells but is not expressed in monocytic cell line.

Negative control : THP-1 (PMID : 10037686).

All lanes:

Western blot - Anti-TNFSF18/GITRL antibody [EPR20583] (<a href='/en-us/products/primary-antibodies/tnfsf18-gitrl-antibody-epr20583-ab211037'>ab211037</a>) at 1/5000 dilution

Lane 1:

HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 10 µg

Lane 2:

THP-1 (Human monocytic leukemia cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 23 kDa

Observed band size: 20 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20583

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251524 is the carrier-free version of ab211037.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that binds to TNFRSF18/AITR/GITR. Regulates T-cell responses. Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B. Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1 (PubMed : 23892569). Promotes leukocyte adhesion to endothelial cells (PubMed : 23892569). Regulates migration of monocytes from the splenic reservoir to sites of inflammation (By similarity).
See full target information TNFSF18
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com