Mouse Monoclonal TRAP/CD40L antibody. Carrier free. Suitable for Flow Cyt, Protein Array, ICC/IF, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD40LG aa 100 to C-terminus.
Images
![Flow Cytometry - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (AB268270), expandable thumbnail](https://content.abcam.com/products/images/tnfsf5-antibody-cd40lg-2763-bsa-and-azide-free-ab268270--flow-cytometry-img80895.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (AB268270)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (AB268270), expandable thumbnail](https://content.abcam.com/products/images/tnfsf5-antibody-cd40lg-2763-bsa-and-azide-free-ab268270--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19919.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (AB268270)")
![Immunocytochemistry/ Immunofluorescence - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (AB268270), expandable thumbnail](https://content.abcam.com/products/images/tnfsf5-antibody-cd40lg-2763-bsa-and-azide-free-ab268270--immunocytochemistry-immunofluorescence-img61204.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (AB268270)")
![Protein Array - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (AB268270), expandable thumbnail](https://content.abcam.com/products/images/tnfsf5-antibody-cd40lg-2763-bsa-and-azide-free-ab268270--protein-array-img138742.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (AB268270)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (AB268270), expandable thumbnail](https://content.abcam.com/products/images/tnfsf5-antibody-cd40lg-2763-bsa-and-azide-free-ab268270--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111775.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (AB268270)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human CD40LG aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P29965
Reactivity data
Select an application| Flow Cyt | Protein Array | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg for 106 Cells | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973). Costimulates T-cell proliferation and cytokine production (PubMed:8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity). CD40 ligand, soluble form. Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
Alternative names
CD154, CD40L, TNFSF5, TRAP, CD40LG, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5
Recommended products
Mouse Monoclonal TRAP/CD40L antibody. Carrier free. Suitable for Flow Cyt, Protein Array, ICC/IF, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD40LG aa 100 to C-terminus.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human CD40LG aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P29965
- Carrier free
- Yes
- Clone number
- CD40LG/2763
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (ab268270), expandable thumbnail](https://content.abcam.com/products/images/tnfsf5-antibody-cd40lg-2763-bsa-and-azide-free-ab268270--flow-cytometry-img80895.jpg "Flow Cytometry - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (ab268270)")
Flow Cytometry - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (ab268270)
Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling TNFSF5 using Anti-TNFSF5 antibody [CD40LG/2763] ab268146 at 2 μg/million cells followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-TNFSF5 antibody [CD40LG/2763] ab268146)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (ab268270), expandable thumbnail](https://content.abcam.com/products/images/tnfsf5-antibody-cd40lg-2763-bsa-and-azide-free-ab268270--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19919.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (ab268270)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (ab268270)
Formalin-fixed, paraffin-embedded human spleen tissue stained for TNFSF5 using Anti-TNFSF5 antibody [CD40LG/2763] ab268146 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-TNFSF5 antibody [CD40LG/2763] ab268146)
![Immunocytochemistry/ Immunofluorescence - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (ab268270), expandable thumbnail](https://content.abcam.com/products/images/tnfsf5-antibody-cd40lg-2763-bsa-and-azide-free-ab268270--immunocytochemistry-immunofluorescence-img61204.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (ab268270)")
Immunocytochemistry/ Immunofluorescence - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (ab268270)
Immunofluorescence staining of Jurkat (Human T cell leukemia cell line from peripheral blood) cells stained for TNFSF5 with Anti-TNFSF5 antibody [CD40LG/2763] ab268146 at 2 μg/ml followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-TNFSF5 antibody [CD40LG/2763] ab268146)
![Protein Array - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (ab268270), expandable thumbnail](https://content.abcam.com/products/images/tnfsf5-antibody-cd40lg-2763-bsa-and-azide-free-ab268270--protein-array-img138742.jpg "Protein Array - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (ab268270)")
Protein Array - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (ab268270)
Protein Array containing more than 19,000 full-length human proteins using Anti-TNFSF5 antibody [CD40LG/2763] ab268146.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-TNFSF5 antibody [CD40LG/2763] ab268146)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (ab268270), expandable thumbnail](https://content.abcam.com/products/images/tnfsf5-antibody-cd40lg-2763-bsa-and-azide-free-ab268270--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111775.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (ab268270)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNFSF5 antibody [CD40LG/2763] - BSA and Azide free (ab268270)
Formalin-fixed, paraffin-embedded human spleen tissue stained for TNFSF5 using Anti-TNFSF5 antibody [CD40LG/2763] ab268146 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-TNFSF5 antibody [CD40LG/2763] ab268146)
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