AB205925

Anti-TNIP2 antibody [EPR17434]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal TNIP2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

ABIN2, FLIP1, TNIP2, TNFAIP3-interacting protein 2, A20-binding inhibitor of NF-kappa-B activation 2, Fetal liver LKB1-interacting protein, ABIN-2

6 Images

Flow Cytometry (Intracellular) - Anti-TNIP2 antibody [EPR17434] (AB205925)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-TNIP2 antibody [EPR17434] (AB205925)

Intracellular Flow Cytometry analysis ofJurkat cells labelling TNIP with ab203829 at 1/90 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Fluorr^® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Immunocytochemistry/ Immunofluorescence - Anti-TNIP2 antibody [EPR17434] (AB205925)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TNIP2 antibody [EPR17434] (AB205925)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling TNIP2 with ab205925 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear and cytoplasmic staining on Jurkat cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab205925 at 1/50 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TNIP2 antibody [EPR17434] (AB205925)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling TNIP2 with ab205925 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear and cytoplasmic staining on HepG2 cell line.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab205925 at 1/50 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-TNIP2 antibody [EPR17434] (AB205925)

TNIP2 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab205925 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab205925 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HeLa whole cell lysate 10ug (Input).

Lane 2 : ab205925 IP in HeLa whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab205925 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-TNIP2 antibody [EPR17434] (ab205925)

Predicted band size: 49 kDa

false

Supplier Data

Western blot - Anti-TNIP2 antibody [EPR17434] (AB205925)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TNIP2 antibody [EPR17434] (ab205925) at 1/1000 dilution

All lanes:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 49 kDa

Observed band size: 49 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-TNIP2 antibody [EPR17434] (AB205925)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TNIP2 antibody [EPR17434] (ab205925) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 49 kDa

Observed band size: 49 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17434

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/90", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits NF-kappa-B activation by blocking the interaction of RIPK1 with its downstream effector NEMO/IKBKG. Forms a ternary complex with NFKB1 and MAP3K8 but appears to function upstream of MAP3K8 in the TLR4 signaling pathway that regulates MAP3K8 activation. Involved in activation of the MEK/ERK signaling pathway during innate immune response; this function seems to be stimulus- and cell type specific. Required for stability of MAP3K8. Involved in regulation of apoptosis in endothelial cells; promotes TEK agonist-stimulated endothelial survival. May act as transcriptional coactivator when translocated to the nucleus. Enhances CHUK-mediated NF-kappa-B activation involving NF-kappa-B p50-p65 and p50-c-Rel complexes.

See full target information TNIP2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:10215-10226 PubMed35443851

2022

Long non-coding RNA-small nucleolar RNA host gene 7 regulates inflammatory responses following spinal cord injury by regulating the microRNA-449a/TNF-α-induced protein 3-interacting protein 2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chunlei He,Jianhua Xiao,Yongjun Ye,Shiqiao Huang,Yanchun Zhong,Lulin Liu,Wuyang Liu,Sheng Liu

World journal of urology : PubMed32666225

2020

PD-L1 expression and pattern of immune cells in pre-treatment specimens are associated with disease-free survival for HR-NMIBC undergoing BCG treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Mathieu Roumiguié,Eva Compérat,Léonor Chaltiel,François Xavier Nouhaud,Gregory Verhoest,Alexandra Masson-Lecomte,Pierre Colin,François Audenet,Nadine Houédé,Stéphane Larré,Evanguelos Xylinas,Serge Brunelle,Jeanne Piana-Thomassin,Juliette Cotte,Géraldine Pignot,Yann Neuzillet,Morgan Rouprêt

Experimental and therapeutic medicine 19:3060-3068 PubMed32256793

2020

microRNA-15a-5p participates in sepsis by regulating the inflammatory response of macrophages and targeting TNIP2.

Applications

Unspecified application

Species

Unspecified reactive species

Yufeng Lou,Zhenrong Huang

Nature communications 10:1858 PubMed31015415

2019

Excessive miR-25-3p maturation via N-methyladenosine stimulated by cigarette smoke promotes pancreatic cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jialiang Zhang,Ruihong Bai,Mei Li,Huilin Ye,Chen Wu,Chengfeng Wang,Shengping Li,Liping Tan,Dongmei Mai,Guolin Li,Ling Pan,Yanfen Zheng,Jiachun Su,Ying Ye,Zhiqiang Fu,Shangyou Zheng,Zhixiang Zuo,Zexian Liu,Qi Zhao,Xu Che,Dan Xie,Weihua Jia,Mu-Sheng Zeng,Wen Tan,Rufu Chen,Rui-Hua Xu,Jian Zheng,Dongxin Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TNIP2 antibody [EPR17434]

Flow Cytometry (Intracellular) - Anti-TNIP2 antibody [EPR17434] (AB205925)

Immunocytochemistry/ Immunofluorescence - Anti-TNIP2 antibody [EPR17434] (AB205925)

Immunocytochemistry/ Immunofluorescence - Anti-TNIP2 antibody [EPR17434] (AB205925)

Immunoprecipitation - Anti-TNIP2 antibody [EPR17434] (AB205925)

Western blot - Anti-TNIP2 antibody [EPR17434] (AB205925)

Western blot - Anti-TNIP2 antibody [EPR17434] (AB205925)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Assay Kits

Product promise